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Foi avaliado o tratamento homeopático de um felino com doença renal policística, moléstia hereditária muito comum na espécie felina. Por ser progressiva, o prognóstico depende do estágio de evolução da doença renal crônica, da resposta do felino ao tratamento inicial e do desejo do tutor em dar continuidade ao tratamento. A observação teve duração de 9 (nove) meses e a avaliação dos resultados foi realizada com o emprego de exame clínico e de exames complementares como imagens ultrassonográficas e exames laboratoriais. A terapia foi composta por medicamento homeopático constitucional Phosphorus 30CH e episódico Kresolum 6CH associado à introdução de dieta renal (ração seca adjuvante). O medicamento homeopático não provocou eventos adversos no animal. Os resultados sugerem que um animal com doença policística felina pode ser tratado pela homeopatia.(AU)

The homeopathic treatment of a feline with polycystic kidney disease was evaluated, a common hereditary disease in the feline species. Because it is progressive, the prognosis depends on the stage of evolution of the chronic kidney disease, the feline response to the initial treatment and the guardian's desire to continue the treatment. The observation lasted 9 (nine) months and the evaluation of the results was performed through clinical examination and complementary tests such as ultrasound images and laboratory tests. The therapy was composed of constitutional Phosphorus 30CH and episodic Kresolum 6CH homeopathic medication associated with the introduction of diet kidney diet (adjunct dry feed). The homeopathic medicine did not cause adverse events in the animal. The results suggest that an animal with feline polycystic disease can be treated by homeopathy.(AU)

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For this study, ½ blood Nelore × Aberdeen Angus heifers (n = 40; 14 months) were divided into two groups: control (n=20; 310 kg) and Pró-Cio (n=20; 304 kg). For Pró-Cio group, 20g of homeopathic product (Pró-cio®, Real H, Campo Grande, Brazil) was supplied together with corn eighteen days prior (D-18) to the beginning of the fixed-time artificial insemination protocol (FTAI). On D0, all females received an intravaginal P4 device and 2 mg BE. On D5, the AFC was assessed by ultrasound and blood was collected for AMH dosage. On D8, P4 device was removed; 300 IU eCG, 0.530 mg of cloprostenol sodium and 0.5 mg of EC were administered; an estrus-identifying adhesive was fixed at the tail insertion and the diameter of the dominant follicle (DF) was measured. On D10, all heifers were inseminated, estrus manifestation was assessed and the preovulatory follicle (POF) was measured. On D20, the CL area was measured and blood flow was assessed using Doppler ultrasound. Data were analyzed by Student, Mann-Whitney or Friedman test. The rates of heat expression, ovulation, and blood flow score with Doppler were analyzed using Fisher's exact test and pregnancy rate by binary logistic regression model (P ≤ 0.05). There was no difference in AFC or AMH dosage (P > 0.05). Pró-Cio group had greater diameters of the DF (9.94 ± 0.42 mm) and POF (11.61 ± 0.56 mm) than the controls (DF: 7.72 ± 0.34 mm and POF: 9.91 ± 0.37 mm), as well a larger CL area (3.26 ± 0.26 versus 2.35 ± 0.16 cm2) and a higher mean CL vascularization score (3.06 versus 2.26; P < 0.05). There was no significant difference (P > 0.05) between estrus manifestation or conception rate for control and Pró-Cio groups. Heifers supplemented with the homeopathic product showed larger follicular diameters at the end of the FTAI protocol and CL with better blood flow scores compared to the control group.

Para esse estudo, novilhas ½ sangue Nelore x Aberdeen Angus (n = 40; 14 meses) foram divididas em dois grupos: Controle (n = 20; 310 kg) e Pró-cio (n = 20; 304 kg). Para o grupo Pró-Cio, 20 g/animal de produto homeopático (Pró-cio®, Real H, Campo Grande, Brazil) foi fornecido juntamente com o milho dezoito dias antes (D-18) de inicar o protocolo de inseminaçãoa artificial em tempo-fixo (IATF). No D0, todas as fêmeas receberam um dispositivo intravaginal de P4 e 2 mg BE. No D5, avaliou-se a contagem de folículos antrais (CFA) por ultrassonografia e realizou-se coleta de sangue para dosagem de AMH. No D8, procedeu-se a retirada do dispositivo de P4; aplicação de 300 UI de eCG, 0,530 mg de cloprostenol sódico e 0,5 mg de CE; um adesivo identificador de estro foi fixado na inserção da cauda e foi feita a mensuração do diâmetro do folículo dominante (FD). No D10, todas as novilhas foram inseminadas, foi realizada a avaliação da manifestação de estro e mensuração do folículo pré-ovulatório (FPO). No D20, realizou-se mensuração da área e avaliação do fluxo sanguíneo do CL por ultrassonografia Doppler. Os dados foram analisados pelos testes de Student, Mann-Whitney ou Friedman. As taxas de expressão de estro, ovulação e escore de fluxo sanguíneo com Doppler foram analisadas usando o teste exato de Fisher e taxa de prenhez pelo modelo de regressão logística binária (p ≤ 0,05). Não houve diferença na CFA nem na dosagem de AMH (p > 0,05). O grupo PróCio apresentou maior diâmetro do FD (9,94 ± 0,42 mm) e FPO (11,61 ± 0,56 mm) em relação ao controle (FD: 7,72 ± 0,34 mm e FPO: 9,91 ± 0,37 mm), além de maior área do CL (3,26 ± 0,26 versus 2,35 ± 0,16 cm2; p < 0,05) e maior escore de vascularização médio do CL (3,06 versus 2,26; p < 0,05). Não houve diferença (p > 0,05) na taxa de manifestação de estro e nem na taxa de concepção entre os grupos Controle e Pró-Cio, respectivamente. Novilhas suplementadas com o produto homeopático apresentaram maiores diâmetros foliculares ao final do protocolo de IATF e CL com melhores escores de fluxo sanguíneo em relação ao grupo controle.

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BACKGROUND: Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. METHODS: Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. RESULTS: The frequency of S. aureus isolations was similar in treated and untreated cows, while 71.4% of the coagulase-negative identified were isolated in cows treated with homeopathy. The distribution of the operon ica genes was similar in animals with and without treatment, except for the icaD gene, more frequent in treated cows. Production of biofilm was associated with presence of one or more genes from the icaADBC operon. S. aureus revealed a greater diversity and greater dissemination in cows treated and not treated with homeopathy. Sequence Types ST1, ST5, and ST126 were identified in S. aureus. CONCLUSIONS: The presence of biofilm-associated genes and the in vitro production of biofilms, combined with the persistence of clonal profiles of Staphylococcus spp. demonstrate other forms of control for bovine mastitis should be researched for organic production herds.

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BACKGROUND: Myxomatous mitral valve disease (MMVD) is the most common cardiopathy in middle-aged dogs. When the dog is asymptomatic and has an enlarged left atrium, treatment is beneficial; however, some allopathic drugs are very costly and may produce side effects. To extend the duration of this asymptomatic phase, complementary therapies such as herbal medicine and homeopathy are available. Although herbal therapy with extract of Crataegus oxyacantha is beneficial, there is a risk of adverse reactions-unlike with homeopathy, where the risk is minimized with the administration of ultra-diluted doses. OBJECTIVE: This study evaluated the efficacy of Crataegus oxyacantha, as mother tincture (MT) and in 6 cH homeopathic formulation, in treating the initial phase of heart failure due to MMVD in a veterinary clinic setting. METHODS: A total of 30 dogs with MMVD, 7 years or older and weighing up to 10 kg, were randomized into three groups as follows: Crataegus 6 cH, Crataegus MT, and hydroalcoholic solution (placebo). Animals were evaluated through echocardiography parameters, laboratory blood tests, and systolic blood pressure (SBP) measurements at 30, 60, 90, and 120 days after initiation of therapy, for statistical analysis and monitoring of the blinded study. RESULTS: Patients who received Crataegus 6 cH showed a reduction in SBP 60 days after treatment, while those receiving Crataegus MT exhibited a reduction 90 days after the therapy was initiated. There was a significant linear regression when evaluating the effect of treatment with Crataegus 6 cH on SBP measurements over the evaluation intervals (linear equation: SBP = 176.57 mm Hg - 0.21x, where x represents days of treatment). There was an increase in both fractional shortening and isovolumetric relaxation time for those patients receiving the homeopathic formulation. CONCLUSIONS: Therapy with Crataegus was beneficial for hypertensive and cardiopathic dogs with MMVD, extending the duration of the asymptomatic phase. The reduction in SBP occurred more swiftly in the 6 cH group than in the MT-treated dogs.

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Acompanhar a evolução do tratamento de um suíno miniatura fêmea de 3 anos que apresentava lesões vesiculares em língua e lábios, região abdominal e casco, bem como anorexia, adpsia, aquesia e vômitos com sangue. Verificou-se desconforto e vocalização intensos ao tentar se locomover, também vocalizava ao deitar com tremores em membros, ficava ofegante, não conseguindo deitar sozinha, precisando de auxílio dos tutores. Com melhora gradativa a partir do 2° dia de tratamento com Arsenicum album 6 CH. Posteriormente foram administrados outros medicamentos (Calcarea carbonica 12 CH e Pulsatilla nig 6 CH e 12 CH), até culminar com Alumina 30 CH e 24 CH, com variação na diluição dessas potências onde se verificou a solução do processo. O tratamento da psora foi mais lento do que na doença aguda. A abordagem homeopática clássica demonstrou resultado significante no paciente estudado com melhora tanto dos sinais clínicos agudos e crônicos.

To follow the evolution of the treatment of a 3 year old female pig. This animal had vesicular lesions on the tongue and lips, abdominal region and hoof, as well as anorexia, adipose, aquesia and vomiting with blood. There was intense discomfort and vocalization when trying to get around, also vocalized at bedtime with tremors in limbs, was panting, unable to lie alone, needing the help of tutors. With gradual improvement from the 2nd day of treatment with Arsenicum album 6 CH. Subsequently, other drugs (Calcarea carbonica 12 CH and Pulsatilla nig 6 CH and 12 CH) were administered, culminating with 30 CH and 24 CH Alumina, with variation in the dilution of these potencies where the process solution was verified. Treatment of psora was slower than in acute disease. The classic homeopathic approach demonstrated a significant result in the studied patient with improvement of both acute and chronic clinical signs.

Acompanar la evolución del tratamiento de un cerdo hembra de 3 años. Este animal presentaba lesiones vesiculares en lengua y labios, región abdominal y casco, así como anorexia, adpsia, aquia y vómitos con sangre. Se verificó incomodidad y vocalización intensos al intentar moverse, también vocalizaba al acostarse con temblores en miembros, se quedaba jadeante, no consiguiendo acostarse sola, necesitando auxilio de los tutores. Con una mejora gradual a partir del 2° día de tratamiento con Arsenicum album 6 CH.En la mayoría de los casos, se utilizaron otros medicamentos (Calcarea carbónica 12 CH y Pulsatilla nig 6 CH y 12 CH), hasta culminar con Alumina 30 CH y 24 CH, con variación en la dilución de esas potencias donde se verificó la solución del proceso. El tratamiento de la psora fue más lento que en la enfermedad aguda. El abordaje homeopático clásico demostró un resultado significante en el paciente estudiado con mejoría tanto de las signos clínicos agudos y crónicos.

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Acompanhar a evolução do tratamento de um suíno miniatura fêmea de 3 anos que apresentava lesões vesiculares em língua e lábios, região abdominal e casco, bem como anorexia, adpsia, aquesia e vômitos com sangue. Verificou-se desconforto e vocalização intensos ao tentar se locomover, também vocalizava ao deitar com tremores em membros, ficava ofegante, não conseguindo deitar sozinha, precisando de auxílio dos tutores. Com melhora gradativa a partir do 2° dia de tratamento com Arsenicum album 6 CH. Posteriormente foram administrados outros medicamentos (Calcarea carbonica 12 CH e Pulsatilla nig 6 CH e 12 CH), até culminar com Alumina 30 CH e 24 CH, com variação na diluição dessas potências onde se verificou a solução do processo. O tratamento da psora foi mais lento do que na doença aguda. A abordagem homeopática clássica demonstrou resultado significante no paciente estudado com melhora tanto dos sinais clínicos agudos e crônicos.(AU)

To follow the evolution of the treatment of a 3 year old female pig. This animal had vesicular lesions on the tongue and lips, abdominal region and hoof, as well as anorexia, adipose, aquesia and vomiting with blood. There was intense discomfort and vocalization when trying to get around, also vocalized at bedtime with tremors in limbs, was panting, unable to lie alone, needing the help of tutors. With gradual improvement from the 2nd day of treatment with Arsenicum album 6 CH. Subsequently, other drugs (Calcarea carbonica 12 CH and Pulsatilla nig 6 CH and 12 CH) were administered, culminating with 30 CH and 24 CH Alumina, with variation in the dilution of these potencies where the process solution was verified. Treatment of psora was slower than in acute disease. The classic homeopathic approach demonstrated a significant result in the studied patient with improvement of both acute and chronic clinical signs.(AU)

Acompanar la evolución del tratamiento de un cerdo hembra de 3 años. Este animal presentaba lesiones vesiculares en lengua y labios, región abdominal y casco, así como anorexia, adpsia, aquia y vómitos con sangre. Se verificó incomodidad y vocalización intensos al intentar moverse, también vocalizaba al acostarse con temblores en miembros, se quedaba jadeante, no consiguiendo acostarse sola, necesitando auxilio de los tutores. Con una mejora gradual a partir del 2° día de tratamiento con Arsenicum album 6 CH.En la mayoría de los casos, se utilizaron otros medicamentos (Calcarea carbónica 12 CH y Pulsatilla nig 6 CH y 12 CH), hasta culminar con Alumina 30 CH y 24 CH, con variación en la dilución de esas potencias donde se verificó la solución del proceso. El tratamiento de la psora fue más lento que en la enfermedad aguda. El abordaje homeopático clásico demostró un resultado significante en el paciente estudiado con mejoría tanto de las signos clínicos agudos y crónicos.(AU)

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Copaifera multijuga, commonly known as copaiba, is popularly used in the form of tea for various conditions due to the presence of antioxidant substances in its composition, which protect cells against damage caused by free radicals. Its oleoresin is also used as an anti-inflammatory and antitumoral agent. The present study investigated the antioxidant effect of the ethanolic extract of copaiba stem bark on Swiss mice inoculated with solid Ehrlich tumors. Mice were inoculated subcutaneously with 1x106 Ehrlichs tumor cells and treated via gavage with ethanolic extract of copaiba for thirty days, with doses varying between 100 and 200 mg kg-1. Biochemical analyses of enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST)], non-enzymatic antioxidants [reduced glutathione (GSH) and ascorbic acid (ASA)], substances reactive to thiobarbituric acid (TBARS) and protein carbonylation (carbonyl) in different tissues were significantly affected. The extract administered at 200 mg kg-1 presented higher antioxidant capacity in the liver, increased CAT, GST, GSH and decreased TBARS, as well as increased CAT activity and protein carbonylation in brain tissue. The results showed that the copaiba extract was able to reverse the oxidative stress caused by solid Ehrlich tumor, probably due to the presence of antioxidant compounds, and had potential antineoplasic effect after a 30-day treatment.(AU)

Copaifera multijuga, ou copaíba, é utilizada popularmente como chá para o tratamento de diversas afecções, o que se deve à presença de substâncias antioxidantes em sua composição, que protegem as células contra danos causados pelos radicais livres. O óleo-resina da árvore é usado como antiinflamatório e antitumoral. O presente estudo avaliou o efeito antioxidante do extrato etanólico da casca da copaíba sobre camundongos Swiss machos inoculados com tumor sólido de Ehrlich. Os camundongos foram inoculados subcutaneamente com 1x106 células de tumor de Ehrlich e foram tratados, via gavagem durante 30 dias, com doses de extrato etanólico de copaíba variando de 100 a 200 mg kg-1. Realizou-se análise bioquímica dos antioxidantes enzimáticos [superóxido dismutase (SOD), catalase (CAT), glutationa-S-transferase (GST)], antioxidantes não-enzimáticos [glutationa reduzida (GSH) e ácido ascórbico (ASA)], substâncias reativas ao ácido tiobarbitúrico (TBARS) e carbonilação proteica (carbonil) em diferentes tecidos e apresentando resultados significativos. O extrato administrado na concentração de 200 mg kg-1 apresentou melhor capacidade antioxidante no fígado, aumentando a CAT, GST, GSH e diminuindo TBARS, além de aumentar a atividade da CAT e da carbonilação proteica no tecido cerebral. Os resultados mostram que o extrato de copaíba foi capaz de reverter o estresse oxidativo causado pelo tumor sólido de Ehrlich, provavelmente por conter compostos antioxidantes, e possivelmente teve um efeito antineoplásico após 30 dias de tratamento.(AU)

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