Assuntos
Humanos , Feminino , Gravidez , Adolescente , Infecções por Ascaridida/diagnóstico , Neoplasias de Cabeça e Pescoço/diagnóstico , Tiabendazol/uso terapêutico , Biópsia , Levamisol/uso terapêutico , Infecções por Ascaridida/cirurgia , Infecções por Ascaridida/patologia , Infecções por Ascaridida/tratamento farmacológico , Doenças Raras/diagnóstico , Doenças Raras/parasitologia , Diagnóstico Diferencial , Quimioterapia Combinada , Anti-Helmínticos/uso terapêutico , Pescoço/patologiaRESUMO
Recently, DNA barcoding based on the mitochondrial gene cytochrome c oxidase subunit 1 (COI) has become a widespread tool to identify animals. Its use with parasites of humans has been limited with some groups of nematodes where the amplification of this gene has been difficult. In this study, we present the first COI barcode sequence of a rare parasite from tropical regions, Lagochilascaris minor, which parasitized a human host from Quintana Roo, southern Yucatán Peninsula, Mexico. Destruction of the mastoid apophysis in the lateral sinus and cerebellar involvement were observed at the site of infection. After a radical mastoidectomy and a treatment with 200 mg oral albendazole for 63 days, the patient completely recovered. Lagochilascaris minor was identified based on the ratio between length of spicules and ejaculatory duct, shape of eggs, and host, as well as comparison with its congeners. The mode of infection is unknown, although it could be after direct exposure to eggs or consumption of uncooked wild meat. Morphology of adults is demonstrated using scanning electron microscopy, and high-quality sequences of COI barcode are presented from amplifications using semi-degenerate primers designed for micro-crustaceans. DNA barcoding proved to be a reliable identification method for L. minor. A comparison of the sequences for this species with 81 ascaridoids obtained from the Barcode of Life Database places it in a unique clade most closely related to Baylisascaris procyonis. Future diagnosis of larval and adult stages of L. minor using DNA barcoding will allow the recognition of its infection parameters, transmission, and precise epidemiology. Reports of lagochilascarosis in the Yucatán Peninsula have been occurred over the last decade, suggesting it is an emerging zoonotic disease in the region.
Assuntos
Infecções por Ascaridida/diagnóstico , Ascaridoidea/classificação , Código de Barras de DNA Taxonômico , Complexo IV da Cadeia de Transporte de Elétrons/genética , Genes Mitocondriais , Animais , Infecções por Ascaridida/diagnóstico por imagem , Infecções por Ascaridida/parasitologia , Infecções por Ascaridida/cirurgia , Ascaridoidea/enzimologia , Ascaridoidea/genética , Ascaridoidea/ultraestrutura , Feminino , Humanos , Funções Verossimilhança , Masculino , México , Microscopia Eletrônica de Varredura , Tomografia Computadorizada por Raios X , Adulto JovemRESUMO
Lagochilascariosis is a zoonotic disease caused by the nematode Lagochilascaris sp., with the northern of Brazil representing 81.2% of all reports of the disease worldwide. The aim of this study was to report the first occurrence of feline lagochilascariosis in the State of Rio Grande do Sul, southern of Brazil. It was diagnosed through coproparasitologic exam and laboratorial identification of the nematodes.
Assuntos
Infecções por Ascaridida/veterinária , Ascaridídios/classificação , Doenças do Gato/parasitologia , Animais , Antiparasitários/uso terapêutico , Infecções por Ascaridida/tratamento farmacológico , Infecções por Ascaridida/epidemiologia , Infecções por Ascaridida/parasitologia , Infecções por Ascaridida/cirurgia , Brasil/epidemiologia , Doenças do Gato/tratamento farmacológico , Doenças do Gato/epidemiologia , Doenças do Gato/cirurgia , Gatos , Feminino , Ivermectina/uso terapêutico , MasculinoRESUMO
An 11-year-old girl presented from Villa Azueta, Veracruz, a poor and small town on the Tesechoacan riverside. The Tesechoacan riverside is a tropical area in the south-east of Mexico, between 18 degrees, 04',32" latitude and 95 degrees, 42',23" longitude. Her family consisted of both parents and 12 brothers, all apparently healthy and living in a humble hut, with poor nutrition and hygiene. The main sign was a tumor on her neck that had developed during the previous 6 months. On clinical examination, the patient's temperature was normal and her weight was 27.5 kg. Below the right angle of the jaw a firm-to-hard tumor was noted measuring 5 x 3 cm, it was tender and lobulated (Fig. 1) with a central purulent fistula containing small worms with morphology of Lagochilascans minor (Fig. 2). Iron-deficiency anemia was found with no increase of blood eosinophils and a while blood count of 9000/mm3 with 72% of neutrophils. Roentgenograms of the head and chest were both normal. A stool was negative for parasites; glutamic oxalade and glutamic pyruvate transaminases were normal. Histopathologic examination revealed parakeratosis and mild acanthosis with exocytosis of eosinophils. The superficial dermis showed an inflammatory reaction, mainly composed of eosinophils, plasma cells, and histiocytes, and in the deep dermis an abscess containing different sections of the parasite was seen surrounded by a thin fibrous capsule (Fig. 3). There was no clinical improvement after medical treatment with two courses of thiabendazole (50 mg/kg orally, two 5-day pulses) and praziquantel (400 mg orally for 3 days). Therefore, the growth was extirpated under general anesthesia; however, the clinical condition of the patient did not improve. Eggs, larvae, and adult worms continued to be present in the pus after surgical excision. Finally, the patient asked for a discharge and did not return to the institute.