RESUMO
This case reports an outbreak of psittacine beak and feather disease in imported budgerigars (Melopsittacusundulatus) in a breeding site in Mexico. Feather abnormalities occurred in 3-month-old budgerigars and the percentage of affected birds was 40 of 100 birds (40%). The disease begins with varying degrees of bare skin on the back, the ventral region of the body, and the thoracic and pelvic regions without clinical signs or mortality offspring. Thirty-tree budgerigars were referred to the laboratory for diagnostics work-up. The hemogram showed different stages of inflammation in all studied birds, where 66% had relative erythrocytosis, leukocytosis due to heterophilia and monocytosis associated with chronic inflammation. The biochemical analysis showed mainly hypoproteinemia and hypouricemia in 5/8 and 4/9 birds, respectively. The absence of feathers with some tiny calamus of the pectoral region was the most significant feature and no other significant pathologic changes were observed. Histopathologic findings were apoptosis and a moderate amount of spherical intracytoplasmic basophilic inclusion bodies in clusters in some epithelial cells of feather follicles (12/14 sections examined) and bursa of Fabricius (8/10 sections examined). End-point PCR of bursa of Fabricius, genome sequencing and phylogenetic analysis, confirmed circovirus identification of the psittacine beak and feather disease virus species and belonging to the group 2 previously found in budgerigars. This is the first description of the psittacine beak and feather disease in budgerigars in Mexico showing the need of monitoring health status of companion and free-ranging endemic and endangered psittacine birds in Mexico in order to collaborate with the trade regulation of these bird species.
Assuntos
Animais , Circovirus , Infecções por Circoviridae/epidemiologia , Infecções por Circoviridae/patologia , Infecções por Circoviridae/sangue , Melopsittacus/virologia , Infecções por Circoviridae/veterinária , México/epidemiologiaRESUMO
This case reports an outbreak of psittacine beak and feather disease in imported budgerigars (Melopsittacusundulatus) in a breeding site in Mexico. Feather abnormalities occurred in 3-month-old budgerigars and the percentage of affected birds was 40 of 100 birds (40%). The disease begins with varying degrees of bare skin on the back, the ventral region of the body, and the thoracic and pelvic regions without clinical signs or mortality offspring. Thirty-tree budgerigars were referred to the laboratory for diagnostics work-up. The hemogram showed different stages of inflammation in all studied birds, where 66% had relative erythrocytosis, leukocytosis due to heterophilia and monocytosis associated with chronic inflammation. The biochemical analysis showed mainly hypoproteinemia and hypouricemia in 5/8 and 4/9 birds, respectively. The absence of feathers with some tiny calamus of the pectoral region was the most significant feature and no other significant pathologic changes were observed. Histopathologic findings were apoptosis and a moderate amount of spherical intracytoplasmic basophilic inclusion bodies in clusters in some epithelial cells of feather follicles (12/14 sections examined) and bursa of Fabricius (8/10 sections examined). End-point PCR of bursa of Fabricius, genome sequencing and phylogenetic analysis, confirmed circovirus identification of the psittacine beak and feather disease virus species and belonging to the group 2 previously found in budgerigars. This is the first description of the psittacine beak and feather disease in budgerigars in Mexico showing the need of monitoring health status of companion and free-ranging endemic and endangered psittacine birds in Mexico in order to collaborate with the trade regulation of these bird species.(AU)
Assuntos
Animais , Melopsittacus/virologia , Infecções por Circoviridae/sangue , Infecções por Circoviridae/patologia , Infecções por Circoviridae/epidemiologia , Circovirus , Infecções por Circoviridae/veterinária , México/epidemiologiaRESUMO
Porcine circovirus 3 (PCV-3) prevalence has been minimally investigated in wild boar; dynamics of infection and viral tissue distribution are currently unknown. In this study, serum samples from 518 wild boar (from years 2004 to 2018) were used to study frequency of infection. Also, serum samples from 19 boar captured and recaptured at least two times for a period of time from 1 month to 1 year were collected to determine PCV-3 infection dynamics. Finally, to elucidate PCV-3 DNA organic distribution, sera, different tissues and faeces were obtained from 35 additional wild boar. PCV-3 DNA was extracted and amplified with a conventional PCR. For the PCV-3 PCR-positive sera from the longitudinally sampled and different tissue types, a quantitative PCR was performed. Genome sequence was obtained from a number of PCV-3 PCR-positive samples from different years, different time-points of infection and tissues. Obtained results confirmed the susceptibility of wild boar to the virus, showing high frequency of PCV-3 detection (221 out of 518, 42.66%) and demonstrating circulation at least since 2004. Compiled data indicate the possibility of long-term infections, since 5 out of 10 PCV-3 PCR-positive boars longitudinally sampled showed positivity in samplings separated for more than 5 months. All tested tissue types' harboured PCV-3 genome, with the highest percentage of PCR positivity in submandibular lymph node, tonsil, lung, liver, spleen and kidney. The amount of DNA in all tested PCV-3 PCR-positive samples was moderate to low. All partial and complete PCV-3 sequences obtained from wild boar displayed high nucleotide identity, higher than 98%. In conclusion, this study further confirms that wild boar is susceptible to PCV-3 infection, showing high frequency of detection in this animal species. Furthermore, PCV-3 can be found in different tissues of wild boar and is apparently able to cause persistent infection.
Assuntos
Infecções por Circoviridae/veterinária , Circovirus/isolamento & purificação , Doenças dos Suínos/epidemiologia , Animais , Infecções por Circoviridae/sangue , Infecções por Circoviridae/epidemiologia , Infecções por Circoviridae/virologia , Feminino , Masculino , Reação em Cadeia da Polimerase/veterinária , Prevalência , Estudos Retrospectivos , Espanha/epidemiologia , Suínos , Doenças dos Suínos/sangue , Doenças dos Suínos/virologiaRESUMO
This study aimed to survey captive wild boars for antibodies against Porcine circovirus-2 (PCV-2) in registered farms. Serum samples (n = 1305) were collected from 90-day-old wild boars from 118 farms of the Brazilian South-east region, including the states of Minas Gerais and São Paulo, and South region, including the states of Paraná, Rio Grande do Sul and Santa Catarina. All herds (100%) presented reactive animals, in varying numbers and from low-to-high antibody titres, with the occurrence ranging from 82 to 89%. Considering farms, the average prevalence was of 84.9% (P < 0.05) and ranged from 54.1 to 94.95%. Regarding the geographic regions studied, the prevalence was of 100%, with PCV2 antibodies detected in wild boars of all regions. This study provides the first evidence of PCV2 antibodies in captive wild boars in Brazil.
Assuntos
Infecções por Circoviridae/epidemiologia , Circovirus/isolamento & purificação , Vigilância de Evento Sentinela/veterinária , Agricultura , Animais , Animais Selvagens/virologia , Anticorpos Antivirais/sangue , Brasil/epidemiologia , Infecções por Circoviridae/sangue , Infecções por Circoviridae/virologia , Circovirus/imunologia , Prevalência , Inquéritos e Questionários , Sus scrofa/virologia , SuínosRESUMO
BACKGROUND: Swine infectious pathogens, especially viruses, represent a potential public health risk associated with the use of pig tissues for xenotransplantation in humans. We hypothesized that porcine circovirus type I (PCV-1) may infect human mononuclear cells, resulting in ultrastructural alterations of the target cells. METHODS: Transmission electron microscopy was used for evaluating ultrastructural alterations of human cells exposed to a PCV-infected PK15 cell line. A polymerase chain reaction (PCR) assay and fluorescence in situ hybridization (FISH) were developed for detecting PCV-1 in human mononuclear cells. RESULTS: Morphological alterations of the human T cells exposed to PCV PK15 showed ''boomerang-shaped'' intracytoplasmic inclusions. Nucleocapsids appeared free, close to the nucleus, or contained into cytoplasmic vacuoles. Virions were observed near the surface of the human cells. A considerable number of mature virions and immature forms could be observed in the human cells that had a completely intact nuclear membrane with no alteration in the disposition of chromatin. PCV-1 particles were identified budding into typical Golgi saccules and vacuoles. Virions sized up to 23 nm in diameter, and appeared in the nucleus and in the periphery of the cellular core. PCV-1 infection was detected on CD4+, CD8+, CD14+, CD19+, and CD56+ human cells by PCR assay and FISH. CONCLUSIONS: These results suggest that PCV has the capability of infecting human leukocytes in vitro, and should be considered a potential risk of viral transmission during xenotransplantation.