RESUMO
Sylvilagus floridanus Papillomavirus (SfPV) causes growth of large horn-like tumors on rabbits. SfPV was described in cottontail rabbits (probably Sylvilagus floridanus) from Kansas and Iowa by Richard Shope in 1933, and detected in S. audubonii in 2011. It is known almost exclusively from the US Midwest. We explored the University of Kansas Natural History Museum for historical museum specimens infected with SfPV, using molecular techniques, to assess if additional wild species host SfPV, and whether SfPV occurs throughout the host range, or just in the Midwest. Secondary aims were to detect distinct strains, and evidence for strain spatio-temporal specificity. We found 20 of 1395 rabbits in the KU collection SfPV symptomatic. Three of 17 lagomorph species (S. nuttallii, and the two known hosts) were symptomatic, while Brachylagus, Lepus and eight additional Sylvilagus species were not. 13 symptomatic individuals were positive by molecular testing, including the first S. nuttallii detection. Prevalence of symptomatic individuals was significantly higher in Sylvilagus (1.8%) than Lepus. Half of these specimens came from Kansas, though new molecular detections were obtained from Jalisco-Mexico's first-and Nebraska, Nevada, New Mexico, and Texas, USA. We document the oldest lab-confirmed case (Kansas, 1915), pre-dating Shope's first case. SfPV amplification was possible from 63.2% of symptomatic museum specimens. Using multiple methodologies, rolling circle amplification and, multiple isothermal displacement amplification in addition to PCR, greatly improved detection rates. Short sequences were obtained from six individuals for two genes. L1 gene sequences were identical to all previously detected sequences; E7 gene sequences, were more variable, yielding five distinct SfPV1 strains that differing by less than 2% from strains circulating in the Midwest and Mexico, between 1915 and 2005. Our results do not clarify whether strains are host species specific, though they are consistent with SfPV specificity to genus Sylvilagus.
Assuntos
Papillomavirus de Coelho Cottontail/isolamento & purificação , Infecções por Papillomavirus/veterinária , Coelhos/virologia , Neoplasias Cutâneas/veterinária , Animais , Antígenos Virais/genética , Sequência de Bases , Colorado/epidemiologia , Papillomavirus de Coelho Cottontail/genética , Papillomavirus de Coelho Cottontail/patogenicidade , DNA Viral/genética , DNA Viral/isolamento & purificação , Genes Virais , História do Século XX , História do Século XXI , Especificidade de Hospedeiro , Kansas/epidemiologia , México/epidemiologia , Meio-Oeste dos Estados Unidos/epidemiologia , Dados de Sequência Molecular , Museus , Proteínas Oncogênicas Virais/genética , Infecções por Papillomavirus/epidemiologia , Infecções por Papillomavirus/história , Infecções por Papillomavirus/virologia , Filogenia , Coelhos/classificação , Homologia de Sequência do Ácido Nucleico , Neoplasias Cutâneas/epidemiologia , Neoplasias Cutâneas/história , Neoplasias Cutâneas/virologia , Especificidade da Espécie , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/história , Infecções Tumorais por Vírus/veterinária , Infecções Tumorais por Vírus/virologia , Proteínas Estruturais Virais/genéticaRESUMO
Current initiatives to reduce the high prevalence of nutritional iron deficiency have highlighted the need for reliable epidemiologic methods to assess iron status. The present report describes a method for estimating body iron based on the ratio of the serum transferrin receptor to serum ferritin. Analysis showed a single normal distribution of body iron stores in US men aged 20 to 65 years (mean +/- 1 SD, 9.82 +/- 2.82 mg/kg). A single normal distribution was also observed in pregnant Jamaican women (mean +/- 1 SD, 0.09 +/- 4.48 mg/kg). Distribution analysis in US women aged 20 to 45 years indicated 2 populations; 93% of women had body iron stores averaging 5.5 +/- 3.35 mg/kg (mean +/- 1 SD), whereas the remaining 7% of women had a mean tissue iron deficit of 3.87 +/- 3.23 mg/kg. Calculations of body iron in trials of iron supplementation in Jamaica and iron fortification in Vietnam demonstrated that the method can be used to calculate absorption of the added iron. Quantitative estimates of body iron greatly enhance the evaluation of iron status and the sensitivity of iron intervention trials in populations in which inflammation is uncommon or has been excluded by laboratory screening. The method is useful clinically for monitoring iron status in those who are highly susceptible to iron deficiency.
Assuntos
Anemia Hipocrômica/diagnóstico , Ferro/análise , Receptores da Transferrina/sangue , Transferrina/análise , Adulto , Idoso , Anemia Hipocrômica/tratamento farmacológico , Anemia Hipocrômica/epidemiologia , Anemia Hipocrômica/prevenção & controle , Suplementos Nutricionais , Método Duplo-Cego , Feminino , Humanos , Absorção Intestinal , Ferro/administração & dosagem , Ferro/farmacocinética , Ferro/uso terapêutico , Deficiências de Ferro , Jamaica/epidemiologia , Kansas/epidemiologia , Masculino , Pessoa de Meia-Idade , Gravidez , Complicações Hematológicas na Gravidez/diagnóstico , Complicações Hematológicas na Gravidez/tratamento farmacológico , Complicações Hematológicas na Gravidez/epidemiologia , Complicações Hematológicas na Gravidez/prevenção & controle , Ensaios Clínicos Controlados Aleatórios como Assunto , Valores de Referência , Sensibilidade e Especificidade , Vietnã/epidemiologiaRESUMO
The separation of oral iron doses to conform to cycle of intestinal mucosa turnover has been suggested by some as a method to improve the efficiency of uptake for therapeutic doses of iron. A short-term study in healthy American women failed to confirm a superior absorption of radioiron with a 7-day interval versus everyday administration of a 50-mg iron dose, but the iron status of the experimental subjects may have produced an inappropriate population on which to test a question relevant to Third World populations.
Assuntos
Ferro/administração & dosagem , Administração Oral , Adulto , Anemia Ferropriva/tratamento farmacológico , Anemia Ferropriva/epidemiologia , Anemia Ferropriva/metabolismo , Ensaios Clínicos como Assunto , Países em Desenvolvimento , Relação Dose-Resposta a Droga , Feminino , Humanos , Mucosa Intestinal/metabolismo , Ferro/metabolismo , Ferro/uso terapêutico , Kansas/epidemiologia , MasculinoRESUMO
From October 1988 to April 1989, a large mumps outbreak occurred in Douglas County, Kansas. Of the 269 cases, 208 (77.3%) occurred among primary and secondary school students, of whom 203 (97.6%) had documentation of mumps vaccination. Attack rates were highest for students attending junior high school (8.0%), followed by high school (2.0%) and elementary school (0.7%). A retrospective cohort study conducted at one junior high school with an attack rate of 12.9% did not find age at vaccination or type of vaccine received (single or combined antigen) to be risk factors for vaccine failure. Students vaccinated more than 4 years before the outbreak appeared to have a higher attack rate than those vaccinated more recently (relative risk (RR) = 4.3; 95% confidence interval (CI) = 0.6, 30.0); however, this association did not exist when risk was evaluated based on number of vaccine doses received. Students who had documentation of receiving only one dose of vaccine were at greater risk than those who had received two doses (RR = 5.2; 95% CI = 1.0, 206.2). Overall, vaccine effectiveness among Douglas County junior high school students was estimated to be 83% (95% CI = 57%, 94%). These data suggest that mumps vaccine failure and the failure to vaccinate have contributed to the relative resurgence of mumps observed in the United States since 1986. The recent change in immunization policy to recommend a two-dose schedule of measles-mumps-rubella vaccine should help reduce the occurrence of mumps outbreaks in highly vaccinated populations.