RESUMO
The objective of this study was to categorise diseases associated with FeLV infection in cats. A total of 154 cats were submitted to necropsy, histopathology exam and anti-FeLV immunohistochemistry (IHC), and 83 (50.9â¯%) were IHC FeLV-positive. The cats age means of 4.1 years, including 3.6â¯% kittens, 34.9â¯% junior, 37.4â¯% prime, 18.1â¯% mature, 2.4â¯% senior, 3.6â¯% unknown age. Neoplastic diseases were most prevalent with leukaemia and lymphoma being most predominant, followed by viral diseases, bacterial, trauma, degenerative, intoxications, parasitic, malformation and others. FeLV+ cats were 5.73 times more likely to be diagnosed with neoplasms than other diseases. The odds ratio (OR) of FeLV+ cats developing leukaemia (OR = 7.75) and lymphoma (OR = 6.75) was higher than other neoplasms. FeLV infection was more prevalent in the mixed breed, junior to prime, male, with neoplastic diseases, including leukaemia and lymphoma. Therefore, understanding the diseases associated with FeLV is of paramount importance in Brazil due to its high prevalence, and it may encourage the implementation of prophylactic measures to reduce its dissemination.
Assuntos
Doenças do Gato , Vírus da Leucemia Felina , Leucemia Felina , Gatos , Animais , Vírus da Leucemia Felina/isolamento & purificação , Brasil/epidemiologia , Masculino , Doenças do Gato/virologia , Doenças do Gato/epidemiologia , Feminino , Prevalência , Leucemia Felina/epidemiologia , Leucemia Felina/virologia , Linfoma/epidemiologia , Linfoma/veterinária , Linfoma/virologia , Infecções por Retroviridae/epidemiologia , Infecções por Retroviridae/veterinária , Infecções por Retroviridae/virologia , Imuno-Histoquímica , Neoplasias/epidemiologia , Neoplasias/veterinária , Neoplasias/virologia , Infecções Tumorais por Vírus/veterinária , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/virologiaRESUMO
The feline leukemia virus (FeLV) belongs to the family Retroviridae; it is the first feline retrovirus discovered and one of the agents that has a great impact on cats' health and the ecology of the feline population worldwide. It is associated with the occurrence of several syndromes of fatal diseases, including the development of lymphomas. Studies on FeLV have been reported in Colombia, and most of them have been approached from a clinical point of view. However, only a few studies have focused on the prevalence of the infection, while none have clarified which variant or FeLV viral subgroup is presently circulating in our country. Therefore, the present study investigated the prevalence of the infection associated with the molecular characterization of FeLV present in cats in Aburrá Valley, Colombia. The sampling of privately owned and shelter cats was performed in female (n = 54) and male (n = 46) felines; most of them were seemingly healthy according to the owner's report, with nonspecific clinical history. Immunoassay confirmed that 59.44% (95% confidence interval (CI) = 49.81-69.06%) of felines were FeLV seropositive. The molecular testing of felines using reverse transcription-polymerase chain reaction and sequencing showed that 30% (30/100) of felines were positive, and the most prevalent subgroup in the Aburrá Valley was FeLV-A. In conclusion, the frequency of leukemia virus, as revealed by molecular and serological tests, is one of the highest reported frequencies to date, and a high molecular variation is shown in the Colombian population. More studies on the behaviour of the virus in feline populations in Columbia are warranted to determine its prevalence throughout the country.
Assuntos
Genoma Viral , Genômica , Vírus da Leucemia Felina/genética , Leucemia Felina/epidemiologia , Leucemia Felina/virologia , Animais , Gatos , Colômbia/epidemiologia , Estudos Transversais , Feminino , Variação Genética , Genômica/métodos , Geografia Médica , Vírus da Leucemia Felina/classificação , Leucemia Felina/diagnóstico , Masculino , Filogenia , Reação em Cadeia da Polimerase , PrevalênciaRESUMO
Leukaemia is a haemopoietic neoplasm originating from myeloid or lymphoid precursors in the bone marrow and may be either acute or chronic. These tumours are rare, but occur more frequently in cats because of an association with the feline leukaemia virus (FeLV) and feline immunodeficiency virus (FIV). To the best of our knowledge, no studies conducted in Brazil to date have analysed the association between leukaemia and FeLV and FIV infection in cats. The aim of this study was to perform a histopathological analysis of feline leukaemia and evaluate the association between leukaemia and FeLV and FIV infection in cats. The study evaluated 37 cats with leukaemia diagnosed between 2009 and 2017. The animals underwent necropsy examination, histopathology and immunohistochemistry with anti-FeLV gp70 and anti-FIV p24 gag antibodies. Of the evaluated animals, 54% (20/37) were males and 43.2% (16/37) were females. With respect to the life stage of the animals, 24.3% (9/37) were junior, 32.4% (12/37) were prime, 18.9% (7/37) were mature and 10.8% (4/37) were senior, and five animals were of unknown age. Myeloid leukaemia occurred in 56.8% (21/37) of the cases and lymphocytic leukaemia occurred in 43.2% (16/37) of the cases. Acute leukaemia (73%, 27/37) was more common than chronic leukaemia (27%, 10/37). The positivity for FeLV (78.4%, 29/37) and FIV (16.2%, 6/37) indicated a high association between FeLV infection and tumour development in the study region.
Assuntos
Doenças do Gato/virologia , Leucemia Felina/virologia , Animais , Brasil , Gatos , Feminino , Vírus da Imunodeficiência Felina , Vírus da Leucemia Felina , MasculinoRESUMO
A cross-sectional study was conducted in 274 cats for determination of FeLV antigenemia and FIV seropositivity and factors associated with those infections in cats presented at the Veterinary Hospital of the Santa Catarina State University - UDESC (Brazil). Apparent prevalence for sick cats at the hospital population was 28.41% (95%CI 21.88-34.94%) for FeLV, 7.65% (95%CI 3.71-11.50%) for FIV and 2.18% (95%CI 0.56-5.47%) for both viruses. For healthy cats, the apparent prevalence was 9.89% (95%CI 3.75-16.02%) for FeLV, 2.20% (95%CI 0.34-7.75%) for FIV by immunoassay (ELISA). Average age for FeLV- and FIV-positive individuals was 38.32 and 64.25 months, respectively. Behavior such as aggressiveness and sex (male) were both associated with increased odds of result positivity test for FeLV and FIV; older animals were also associated with FIV test results. A very small proportion of the animals were vaccinated against FeLV and none against FIV. Most of the animals were adopted from shelters or rescued from streets, living with multiple cats that had access to outdoors. The high prevalence of FeLV suggests a need for better control strategies against this disease.
Assuntos
Doenças do Gato/epidemiologia , Síndrome de Imunodeficiência Adquirida Felina/epidemiologia , Vírus da Imunodeficiência Felina/imunologia , Vírus da Leucemia Felina/imunologia , Leucemia Felina/epidemiologia , Infecções Tumorais por Vírus/veterinária , Animais , Antígenos Virais/sangue , Antígenos Virais/imunologia , Comportamento Animal/fisiologia , Brasil/epidemiologia , Doenças do Gato/virologia , Gatos , Estudos Transversais , Ensaio de Imunoadsorção Enzimática/veterinária , Síndrome de Imunodeficiência Adquirida Felina/virologia , Feminino , Leucemia Felina/virologia , Masculino , Prevalência , Fatores de RiscoRESUMO
BACKGROUND: Visceral leishmaniasis (VL) has been increasingly recognized in cats living in areas endemic for the disease. Co-infection with Leishmania infantum and other infectious agents is well established in dogs. However, for cats, data on co-infections with L. infantum and other infectious agents are still sparse. The aim of this study was to identify the prevalence of vector-borne pathogens, Mycoplasma spp., feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) in cats from an area endemic for VL in southeastern Brazil. RESULTS: Of the 90 cats, eight (8.9%) were infected with Mycoplasma spp., five (5.5%) were FIV- positive and one (1.1%) was FeLV-positive. Co-infection with L. infantum and at least one other infectious agent was found in 9/50 (18.0%; CI: 8.6-31.4%) cats. In Group 1 (cats infected naturally by L. infantum), 4/50 (8.0%) cats were positive for FIV, 4/50 (8%) for Mycoplasma spp. and 1/50 (2.0%) was co-infected with FeLV and Mycoplasma spp. In Group 2 (cats non-infected with L. infantum), 2/40 (5.0%) cats were infected with Mycoplasma spp. and 1/40 (2.5%) was co-infected with FIV and Mycoplasma spp. All cats were negative for Ehrlichia spp., Babesia spp. and Anaplasma platys. CONCLUSION: A low prevalence of co-infection in Leishmania-infected and non-infected cats was found. Co-infections with Leishmania and vector-borne diseases in cats are not common in this area endemic for VL in Brazil.
Assuntos
Doenças do Gato/epidemiologia , Doenças Endêmicas/veterinária , Síndrome de Imunodeficiência Adquirida Felina/epidemiologia , Leishmaniose Visceral/veterinária , Leucemia Felina/epidemiologia , Infecções por Mycoplasma/epidemiologia , Anaplasma/isolamento & purificação , Animais , Babesia/isolamento & purificação , Brasil/epidemiologia , Doenças do Gato/microbiologia , Doenças do Gato/virologia , Gatos , Coinfecção/microbiologia , Coinfecção/parasitologia , Coinfecção/veterinária , Síndrome de Imunodeficiência Adquirida Felina/virologia , Vírus da Imunodeficiência Felina/isolamento & purificação , Leishmaniose Visceral/epidemiologia , Leishmaniose Visceral/parasitologia , Vírus da Leucemia Felina/isolamento & purificação , Leucemia Felina/virologia , Mycoplasma/isolamento & purificação , Infecções por Mycoplasma/microbiologia , PrevalênciaRESUMO
Our aims were to determine the frequencies of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) in owned and stray cats in the northeastern region of Brazil, ascertain the status of FeLV infection, and investigate potential associated factors among the owned cats. Blood samples from 200 asymptomatic owned cats and 30 stray cats were processed using nested PCR and commercial immunochromatographic tests to diagnose infections. To evaluate the factors associated with FIV and/or FeLV in owned cats, a semi-structured interview was conducted with each owner about the animal's environment, and these data were subjected to unconditional logistic regression. The frequencies for owned cats were 6% (12/200) and 3% (6/200) for FIV and FeLV, respectively. No owned cat was positive for both viruses. Stray cats showed frequencies of 6.66% (2/30) and 0% (0/30) for FIV and FeLV, respectively. Contact with other cats and living in peri-urban areas were considered to be risk factors (P < 0.05) for FIV. We did not identify any factors associated with infections with FeLV. Our results confirm the presence of these two retroviruses in the region under study. Our use of different diagnostic techniques allowed us to determine the frequency of retroviruses in the feline population more accurately, particularly with regard to infections by FeLV, which have complex pathogenesis.
Assuntos
Gatos/virologia , Síndrome de Imunodeficiência Adquirida Felina/epidemiologia , Vírus da Imunodeficiência Felina/genética , Vírus da Leucemia Felina/genética , Leucemia Felina/epidemiologia , Animais , Brasil , Síndrome de Imunodeficiência Adquirida Felina/virologia , Feminino , Leucemia Felina/virologia , Masculino , Animais de Estimação/virologiaRESUMO
A cross-sectional study was carried out on cats attending the Small Animal Hospital at the Faculty of Veterinary Sciences of the University of Buenos Aires to assess the prevalence and associated risk factors of Feline immunodeficiency virus (FIV) and Feline leukemia virus (FeLV) in the city of Buenos Aires, Argentina. Blood samples from 255 cats with symptoms compatible with FIV or FeLV infection, collected between 2009 and 2013 were analyzed by serology (immunochromatography, IA) and by hemi-nested PCR (n-PCR). The IA and n-PCR assays showed similar percentages of positivity for FIV while the n-PCR test was more sensitive for FeLV. Differences between the diagnostic tests and their choice according to the age of the animal are discussed. The clinical histories of ninety of the 255 cats showed blood profiles similar to others previously reported and revealed a higher risk of infection in male adult cats with outdoor access.
Assuntos
Cromatografia de Afinidade/métodos , Síndrome de Imunodeficiência Adquirida Felina/diagnóstico , Vírus da Imunodeficiência Felina/isolamento & purificação , Vírus da Leucemia Felina/isolamento & purificação , Leucemia Felina/diagnóstico , Reação em Cadeia da Polimerase/métodos , Viremia/diagnóstico , Animais , Argentina/epidemiologia , Gatos/virologia , Estudos Transversais , DNA Viral/análise , Síndrome de Imunodeficiência Adquirida Felina/epidemiologia , Síndrome de Imunodeficiência Adquirida Felina/virologia , Feminino , Vírus da Imunodeficiência Felina/genética , Vírus da Imunodeficiência Felina/imunologia , Vírus da Leucemia Felina/genética , Vírus da Leucemia Felina/imunologia , Leucemia Felina/epidemiologia , Leucemia Felina/virologia , Masculino , Prevalência , Provírus/isolamento & purificação , Kit de Reagentes para Diagnóstico , Fatores de Risco , Sensibilidade e Especificidade , Viremia/epidemiologia , Viremia/virologiaRESUMO
Blood samples from 1,072 domestic cats of nine administrative regions of Belo Horizonte, MG, were collected and tested using PCR nested for the occurrence of feline leukemia virus (FeLV). Overall occurrence was 47.5 percent (507/1072) being North (68.1 percent) and East (54.4 percent) the most prevalent areas. Epidemiological data showed that FeLV infection was very common among examined cats and breed neither gender nor were predisposing factors for FeLV. The results suggest that the agglomeration of a large number of cats in the same environment can be an important factor for the increase in the rate of transmission of this retrovirus among domestic cats in the studied city.(AU)
Assuntos
Animais , Gatos , Leucemia Felina/diagnóstico , Leucemia Felina/virologia , Análise Química do Sangue/veterinária , Sorologia , Testes Sorológicos , Densidade DemográficaRESUMO
Blood samples from 1,072 domestic cats of nine administrative regions of Belo Horizonte, MG, were collected and tested using PCR nested for the occurrence of feline leukemia virus (FeLV). Overall occurrence was 47.5 percent (507/1072) being North (68.1 percent) and East (54.4 percent) the most prevalent areas. Epidemiological data showed that FeLV infection was very common among examined cats and breed neither gender nor were predisposing factors for FeLV. The results suggest that the agglomeration of a large number of cats in the same environment can be an important factor for the increase in the rate of transmission of this retrovirus among domestic cats in the studied city.
Assuntos
Animais , Gatos , Leucemia Felina/diagnóstico , Leucemia Felina/virologia , Análise Química do Sangue/veterinária , Densidade Demográfica , Testes Sorológicos , SorologiaRESUMO
A nested-PCR (n-PCR) was used to detect feline leukemia virus (FeLV) proviral DNA in blood samples from 464 sick and 608 healthy domestic cats (Felis catus) selected by convenience, and a significantly high prevalence of FeLV infection was observed. n-PCR results revealed the presence of FeLV proviral DNA in 47.2 % of sick cats and 47.4 % of healthy cats. Phylogenetic analysis revealed that FeLV samples from healthy or sick cats were grouped into separate clades. We determined FeLV subgroups by an n-PCR based on the envelope (env) gene. The partial env gene of FeLV Minas Gerais (MG) samples were compared to various exogenous FeLV isolates and endogenous (enFeLV) provirus from the same region. FeLV-B MG samples were more similar to endogenous sequences and to natural FeLV-B isolates than to either FeLV-A or FeLV-C. The results revealed the circulation of FeLV-B in large populations of urban domestic cats in Brazil.