RESUMO
The immunomodulatory effect of hydrosoluble extracts of four Chilean Cyttaria species (Discomycetes, Fungi) was assessed in mice with L5178Y lymphoma. Oral administration of 100 mg extract per day for 7 days enhanced the percentual phagocytosis and phagocytosis index in animals receiving Cyttaria berteroi, Cyttaria darwinii, Cyttaria espinosae and Cyttaria harioti extracts. Differences in the digestion index were observed in mice treated with C. darwinii and C. berteroi. In the delayed-type hypersensitivity model, only C. harioti was able to modify the immune response. The results suggest that intake of Cyttaria can improve the immune system of consumers.
Assuntos
Adjuvantes Imunológicos/uso terapêutico , Leucemia L5178/tratamento farmacológico , Fitoterapia , Extratos Vegetais/uso terapêutico , Animais , Chile , Leucemia L5178/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Fagocitose/efeitos dos fármacosAssuntos
Antígenos de Neoplasias/isolamento & purificação , Antígenos/isolamento & purificação , Autoantígenos/isolamento & purificação , Leucemia L5178/imunologia , Leucemia Experimental/imunologia , Ribonucleoproteínas Nucleares Pequenas , Adulto , Animais , Anticorpos Antinucleares/análise , Doenças do Colágeno/diagnóstico , Reações Cruzadas , Diagnóstico Diferencial , Feminino , Humanos , Imunodifusão , Lúpus Eritematoso Sistêmico/diagnóstico , Lúpus Eritematoso Sistêmico/imunologia , Camundongos , Pessoa de Meia-Idade , Proteínas Centrais de snRNPRESUMO
Location of neoplasia specific tumoral antigens (ANE) were investigated in L5178Y murine cells. Cells were treated by means of concanavalin A (CoA), Vibrio cholerae neuraminidase (NVC) or a combination of both (Coa-NVC) and were inactivated by 6,000 rad irradiation. With 5 x 10(6) treated cells four groups, 15 mice in each group of the BALB/c strain were immunized weekly and 10 days latter immunity to L5178Y lymphoma cells was tested by means of intraperitoneal inoculation of 5 x 10(7) intact, viable L5178Y cells. None of the mice immunized with concanavalin A treated and irradiated cells developed the inoculated lymphoma. However all mice immunized with cells treated with CoA or CoA-NVC developed a lymphoma. Electron microscopy studies of normal L5178Y cells, irradiated or treated with NVC showed the exterior cell coating or glycocalix that was absent in those treated with CoA or CoA-CoA-NVC. These results allowed us to conclude that ANE of L5178Y cells susceptible to modification by NVC as if to induce an immunologic rejection response are located in the glycocalix of these cells.