RESUMO

The flowers of the species of Malpighiaceae in the Neotropical Region are relatively uniform in their morphology due to their dependence on oil-collecting bees as their main pollinators. However, many species of the genus Galphimia seem to have acquired a different floral syndrome, lacking markedly zygomorphic flowers and developed elaiophores in the calyx. Likewise, these species present anthers with great development, probably in response to the selection of pollinators that collect pollen. Galphimia australis incorporated some of these traits but also retained some residual characteristics typical of species pollinated by oil bees. This leads to many questions on how these flowers ensure their pollination. Inquiring about the reduction or modification of these characteristics allows us to understand how G. australis achieves a different pollination syndrome. In this research, we carry out a detailed morphological and anatomical study of the flowers and pollen grain devolvement of G. australis and floral visitors were observed and captured. Results were analyzed in order to determine how this species changed from the oil-floral syndrome, typical of neotropical Malpighiaceae, to one syndrome with pollen as the main reward.

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This paper aimed to evaluate crop yield and physical and chemical characteristics of acerola (Malpighia emarginata Sessé & Mociño ex DC) clones grown in the Alta Paulista region, Brazil. The evaluation comprised 7 clones, namely: Olivier, BRS 238-Frutacor, BRS 236-Cereja, BRS 235-Apodi, BRS 237-Roxinha, Okinawa, and Waldy-CATI 30, studiedfrom October 2018 to April 2019. Crop yield was analyzed based on the accumulated production of 12 harvests, with fresh mass expressed in kg fruit·plant-1. Considering the capacity of30 harvests per plant within the complete cropping period, total fruit mass (in kg fruit·plant-1) and crop yield (in tha-1) were estimated. Samples were collected from 4 of the 12 harvests in order to determine average fruit mass (g); average fruit size, fruit height/diameter ratio, and pulp yield (%). Regarding fruit pulp, the analysis consisted of quantifying the soluble solids, expressed in degrees Brix, and the technological index (i.e. pulp yield x °Brix/100). The experiment was conducted in a randomized block design, with 7 treatments and 4 repetitions. The obtained data were statistically processed by analysis of variance using the F test, and the means were compared using the Tukey’stest at the 0.05 significance level. The study concluded that, given the soil and weather conditions of the Alta Paulista region, Olivier, Apodi and Frutacor are the clones mainly recommended for the agro-industrial market, being the Olivier cultivar the first option among the three and appearing in a greater proportion in the area of the commercial orchard studied. Regarding the acerola production for fresh consumption, Olivier and Roxinha are the most suitable clones. Olivier stands out for its good fruit yield, adequate physical and chemical characteristics, and easy manual harvesting.

Objetivou-se avaliar a produtividade e características físico-químicas de clones de acerola cultivados na região Alta Paulista. Avaliou-se 7 clones: Olivier, BRS 238-Frutacor, BRS 236-Cereja, BRS 235-Apodi, BRS 237-Roxinha, Okinawa e Waldy-CATI 30, no período de outubro de 2018 a abril de 2019. Analisou-se produção acumulada de 12 colheitas, sendo expressa a massa fresca em kg de frutos.planta-1. Seguindo a premissa de 30 colheitas por planta ao longo de uma safra completa, estimou-se a produção total de frutos em kg frutos planta-1e a produtividade em t ha-1. Em 4 das 12 colheitas, foram retiradas amostras para avaliação da massa média do fruto (g); tamanho médio do fruto, relação altura: diâmetro e rendimento de polpa (%). Na polpa foram quantificados os sólidos solúveis expressos em ° Brix e o índice tecnológico (rendimento de polpa x °Brix/100). Como delineamento experimental utilizou-se blocos ao acaso, com 7 tratamentos e 4 repetições. Os dados foram submetidos à análise de variância para o Teste F e as médias comparadas pelo Teste Tukey ao nível de 5% de significância. Concluiu-se que nas condições de clima e solo da região Alta Paulista recomenda-se o plantio dos clones Olivier, Apodi e Frutacor principalmente visando o mercado agroindustrial, sendo o clone Olivier a primeira opção e em maior proporção na área de implantação do pomar comercial. No cultivo de acerola voltado para consumo in natura indicam-se clones Olivier e Roxinha. O clone Olivier se destaca pela boa produtividade, características físico-químicas adequadas e facilidade de colheita manual dos frutos.

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RESUMO

This paper aimed to evaluate crop yield and physical and chemical characteristics of acerola (Malpighia emarginata Sessé & Mociño ex DC) clones grown in the Alta Paulista region, Brazil. The evaluation comprised 7 clones, namely: Olivier, BRS 238-Frutacor, BRS 236-Cereja, BRS 235-Apodi, BRS 237-Roxinha, Okinawa, and Waldy-CATI 30, studiedfrom October 2018 to April 2019. Crop yield was analyzed based on the accumulated production of 12 harvests, with fresh mass expressed in kg fruit·plant-1. Considering the capacity of30 harvests per plant within the complete cropping period, total fruit mass (in kg fruit·plant-1) and crop yield (in tha-1) were estimated. Samples were collected from 4 of the 12 harvests in order to determine average fruit mass (g); average fruit size, fruit height/diameter ratio, and pulp yield (%). Regarding fruit pulp, the analysis consisted of quantifying the soluble solids, expressed in degrees Brix, and the technological index (i.e. pulp yield x °Brix/100). The experiment was conducted in a randomized block design, with 7 treatments and 4 repetitions. The obtained data were statistically processed by analysis of variance using the F test, and the means were compared using the Tukeystest at the 0.05 significance level. The study concluded that, given the soil and weather conditions of the Alta Paulista region, Olivier, Apodi and Frutacor are the clones mainly recommended for the agro-industrial market, being the Olivier cultivar the first option among the three and appearing in a greater proportion in the area of the commercial orchard studied. Regarding the acerola production for fresh consumption, Olivier and Roxinha are the most suitable clones. Olivier stands out for its good fruit yield, adequate physical and chemical characteristics, and easy manual harvesting.(AU)

Objetivou-se avaliar a produtividade e características físico-químicas de clones de acerola cultivados na região Alta Paulista. Avaliou-se 7 clones: Olivier, BRS 238-Frutacor, BRS 236-Cereja, BRS 235-Apodi, BRS 237-Roxinha, Okinawa e Waldy-CATI 30, no período de outubro de 2018 a abril de 2019. Analisou-se produção acumulada de 12 colheitas, sendo expressa a massa fresca em kg de frutos.planta-1. Seguindo a premissa de 30 colheitas por planta ao longo de uma safra completa, estimou-se a produção total de frutos em kg frutos planta-1e a produtividade em t ha-1. Em 4 das 12 colheitas, foram retiradas amostras para avaliação da massa média do fruto (g); tamanho médio do fruto, relação altura: diâmetro e rendimento de polpa (%). Na polpa foram quantificados os sólidos solúveis expressos em ° Brix e o índice tecnológico (rendimento de polpa x °Brix/100). Como delineamento experimental utilizou-se blocos ao acaso, com 7 tratamentos e 4 repetições. Os dados foram submetidos à análise de variância para o Teste F e as médias comparadas pelo Teste Tukey ao nível de 5% de significância. Concluiu-se que nas condições de clima e solo da região Alta Paulista recomenda-se o plantio dos clones Olivier, Apodi e Frutacor principalmente visando o mercado agroindustrial, sendo o clone Olivier a primeira opção e em maior proporção na área de implantação do pomar comercial. No cultivo de acerola voltado para consumo in natura indicam-se clones Olivier e Roxinha. O clone Olivier se destaca pela boa produtividade, características físico-químicas adequadas e facilidade de colheita manual dos frutos.(AU)

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Worldwide distributed tropical savannas were established only in the Miocene, with climatic cooling and rise of C4 grasses. However, there is evidence for an earlier presence of savanna-like vegetation in southern parts of South America. Here we investigated the biogeographic history of a clade of solitary bees which have endemic groups in areas covered by savannas and other types of open vegetation as well as forested areas. We hypothesized that these bees originated in savanna-like biomes and that shifts to forested areas and floral host shifts increased species diversification along their evolutionary history. We reconstructed a comprehensive phylogeny for Tapinotaspidini bees based majorly on original DNA sequences. We then used macroevolutionary tools to estimate ancestral range area and reconstructed ancestral habitat (open versus forested) and host plant association to analyze the effects of shifts in vegetation type and flower hosts on their diversification. Tapinotaspidini bees originated in the Paleocene and in a savanna-type, Cerrado-like, which is reinforced by reconstruction of open vegetation as the most probable ancestral area, thus bringing additional evidence to a much earlier origin of this vegetation type in South America. Shifts to forested areas occurred at least three times in a period of 30 Ma and were responsible for slight increases in diversification rates. Malpighiaceae is the ancestral floral host; host broadening occurred only in the Miocene and at least in three occasions. Host shifts, i.e. from Malpighiaceae to other oil families, occurred in the Eocene and Miocene. Both host broadening and host shifts did not significantly alter diversification rates, however exploitation of other oil sources were important in occupying new habitats. The link between biomes and host plant shifts and changes in diversification rate brings us additional insights into the evolution of bees and associated flora in South America.

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O objetivo do presente trabalho foi avaliar a influência da concentração da maltodextrina e da temperatura de entrada de secagem sob as características físico-químicas de polpa de acerola em pó. As acerolas maduras foram selecionadas, lavadas, sanitizadas, enxaguadas e despolpada. As polpas foram divididas em quatro tratamentos, utilizando-se duas concentrações de maltodextrina (14 e 26%) que foram atomizadas em spray dryer em duas temperaturas (128 e 152°C). A polpa em pó e in natura foram analisadas quanto a umidade, SST, ATT, pH e carotenoides. De acordo com os resultados obtidos foi possível verificar que os teores de umidade foram influenciados pela temperatura e concentração de maltodextrina. A acidez da polpa em pó diminui com o aumento da do agente carreador. O teor de carotenoides não apresentou diferença significativa entre os tratamentos estudados. Para o processo de atomização da acerola por spray dryer indica-se o uso de maltodextrina a 26% independente da temperatura.

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RESUMO

O objetivo do presente trabalho foi avaliar a influência da concentração da maltodextrina e da temperatura de entrada de secagem sob as características físico-químicas de polpa de acerola em pó. As acerolas maduras foram selecionadas, lavadas, sanitizadas, enxaguadas e despolpada. As polpas foram divididas em quatro tratamentos, utilizando-se duas concentrações de maltodextrina (14 e 26%) que foram atomizadas em spray dryer em duas temperaturas (128 e 152°C). A polpa em pó e in natura foram analisadas quanto a umidade, SST, ATT, pH e carotenoides. De acordo com os resultados obtidos foi possível verificar que os teores de umidade foram influenciados pela temperatura e concentração de maltodextrina. A acidez da polpa em pó diminui com o aumento da do agente carreador. O teor de carotenoides não apresentou diferença significativa entre os tratamentos estudados. Para o processo de atomização da acerola por spray dryer indica-se o uso de maltodextrina a 26% independente da temperatura.(AU)

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We analyzed the gynoecium morphology and anatomy of Tricomaria usillo in young and mature flowers from diverse populations in order to analyze the differentiation of structure and function of the parts of the carpel. We also aimed to find the potential pollinators and associate the morphology of the gynoecium with its role. We compare the characteristics of the gynoecium of T. usillo and discuss the carpel dimorphism with other genera within the Carolus clade in relation with their pollination syndromes. Carpels were processed according to classic techniques for scanning electron microscopy and bright field microscopy. We conducted field observation in different populations of T. usillo and captured the insects that were identified to specific level. The gynoecium of T. usillo shows inter-population and intra-individual variability. Some have three well-developed carpels, while most of them present two posterior carpels with differentiated styles and stigmas and the anterior one with a shorter style with or without stigma. The ovary has three locules with one ovule each. A compitum is formed and all ovules may be fecundated. However, fruits have generally one seed that develops in the anterior locule. Centris brethesi is the potential pollinator. The gynoecium of T. usillo reflects part of the variation in the carpel dimorphism that probably arose in the branch of the Carolus clade, and evolved in diverse ways in the lineages of this group. Tricomaria usillo seems to represent a recent transition towards reaching a stable form of carpel dimorphism and definitive division of labors of the carpels.

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Long-lived flowers increase pollen transfer rates, but these entail high water and carbon maintenance costs. The retention of pollinated and reward-free old flowers enhances pollinator visitation to young receptive flowers by increasing floral display size. This mechanism is associated with acropetal inflorescences or changes in flower colour and openness, but the retention of unchanging solitary flowers remains overlooked. We examined pollination-dependent variation in floral longevity and determined stigmatic receptivity, pollen viability and pollen removal rates among flower ages in Kielmeyera regalis, a Neotropical savanna shrub. We also evaluated the effects of floral display size on pollinator visitation rates. Lastly, we determined whether old flowers are unvisited and exclusively increase pollinator attraction to young flowers through flower removal experiments. Regardless of pollination treatment, flowers lasted fully open with no detectable physical changes for 3 days. Over time, stigmas remained receptive but >95% of pollen was removed. Pollinator visitation significantly increased with floral display size and intermediate percentages (15-30%) of newly opened flowers. Accordingly, the retention of reward-free and unvisited old flowers increased young flower-pollinator interaction. Our results reveal the importance of a prolonged floral longevity in increasing pollinator attraction toward newly opened receptive flowers without changes in flower colour and form. We conclude that the retention of pollinated, reward-free and unvisited colour-unchanged old flowers in K. regalis is a strategy that counteracts the water use costs associated with the maintenance of large flowers with increased mate opportunities in a pollen-limited scenario.

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This study evaluated the inclusion of increasing contents (0; 35; 70; 105 and 140 g kg-1) of dried acerola fruit (Malpighia glabra, Linn.) by-product (DABP) in the ensilage of elephant grass (Pennisetum purpureum, Schum.) considering the in situ degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and hemicellulose. Plastic drums with 210 L capacity were used as experimental silos. After 45 days of ensiling, silos were opened and silage samples were collected for the degradability trial and laboratory analysis. For the in situ degradability trial, was used one rumen-fistulated adult male cattle. The five by-product inclusion contents were tested in three replicates, with five incubation times, in a randomized complete split-plot design in which the contents of DABP were the treatments (plots), the different silos were the replicates and the incubation times were the sub-plots. Longer incubation times significantly increased (P < 0.05) the disappearance rates of DM, CP, NDF, ADF and hemicellulose. The effective degradability of the DM, NDF and hemicellulose was higher in the silages with 35 g kg-1 of DABP in the rumen passage rates of 0.02; 0.05 and 0.08 h-1. Dried acerola fruit by-product can be added to elephant grass ensiling up to 35 g kg-1 without reducing the effective degradability of nutrients.

O presente estudo avaliou a inclusão de teores crescentes (0; 35; 70; 105 e 140 g kg-1) do subproduto da acerola (Malpighia glabra, Linn.) desidratado (SPAD) na ensilagem do capim elefante (Pennisetum purpureum, Schum.) por meio da degradabilidade in situ da matéria seca (MS), proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA) e hemicelulose. Foram utilizados tambores plásticos de 210 L como silos experimentais. Após 45 dias da ensilagem, os silos foram abertos e coletadas amostras para realização do ensaio de degradabilidade e análises laboratoriais. Para o estudo de degradabilidade in situ foi utilizado um bovino adulto macho, fistulado no rúmen. Os cinco teores do subproduto foram testados com três repetições e cinco tempos de incubação, em delineamento inteiramente casualizado com parcelas subdivididas, sendo os teores de SPAD os tratamentos (parcelas), os diferentes silos as repetições e os tempos de incubação as subparcelas. Os maiores tempos de incubação aumentaram significativamente (P < 0,05) as taxas de desaparecimento da MS, PB, FDN, FDA e hemicelulose. A degradabilidade efetiva da MS, FDN e hemicelulose apresentou maiores valores nas silagens com 35 g kg-1 SPAD nas taxas de passagem ruminais de 0,02; 0,05 e 0,08 h-1. O subproduto da acerola desidratado pode ser adicionado à ensilagem do capim elefante em até 35 g kg-1 sem reduzir a degradabilidade efetiva dos nutrientes.

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