RESUMO
The ultrastructural development of the surface belonging to the epithelium-connective tissue interface of the hard and soft palatine mucosa of mouse was studied using field emission scanning electron microscopy and high voltage transmission electron microscopy methods. The tissues were analysed at 18 days of gestation, and, 1, 3, 7, 14, 21, 30 and 90 days after birth. The three-dimensional architecture of the connective tissue in the lamina propria showed different shapes. The papillae began to form on days 7 and 14 but continued to growth until the adult period. Different stages of primary and secondary connective tissue papillae were recognized in the hard palatine mucosa whereas the soft palate showed erect connective tissue papillae, small round formations containing taste buds and several openings of the salivary glands ducts.
Assuntos
Mucosa Bucal/crescimento & desenvolvimento , Palato Mole/crescimento & desenvolvimento , Palato/crescimento & desenvolvimento , Envelhecimento , Animais , Colágeno/ultraestrutura , Tecido Conjuntivo/ultraestrutura , Epitélio/ultraestrutura , Idade Gestacional , Camundongos , Microscopia Eletrônica , Microscopia Eletrônica de Varredura , Mucosa Bucal/embriologia , Mucosa Bucal/ultraestrutura , Palato/embriologia , Palato/ultraestrutura , Palato Mole/embriologia , Palato Mole/ultraestruturaRESUMO
The three-dimensional structure of the lamina propria of the hard and soft palatine mucosa of the nine-banded armadillo was observed by scanning electron microscopy. Sodium hydroxide cell maceration method was applied to demonstrate the architecture of the connective tissue papillae. The palatine mucosa of the armadillo had a triangular shape and measured appr. 6.5 cm length. The hard palate showed 9 transverse palatine plicae while the soft palate was smooth. In the 10% NaOH treated specimens, the lamina propria of the hard palatine mucosa showed numerous connective tissue papillae with a general finger-like shape. These structures were composed by a meshwork of collagen fibers arranged in several directions. On the other hand, the connective tissue papillae of the soft palate mucosa were scattered and small. Numerous openings of glandular ducts with circular or elliptical shape were located in the interplicae area and in the soft palate.
Assuntos
Tatus/anatomia & histologia , Mucosa Bucal/ultraestrutura , Palato/ultraestrutura , Animais , Colágeno/análise , Feminino , Masculino , Microscopia Eletrônica de Varredura , Palato Mole/ultraestrutura , Glândulas Salivares/ultraestruturaRESUMO
Relato de caso de síndrome maligno-mediofacial de natureza linfomatosa, localizado no palato, em paciente do sexo feminino, com 23 anos de idade. O diagnóstico foi feito por meio do estudo histopatológico e de marcadores imuno-histoquímicos para linfócitos T. Apesar do êxito terapêutico alcançado com a associaçäo de ciclosporina, vincristina, andriamicina e prednisona, após ter tido alta hospitalar, a paciente faleceu, em consequência de septicemia
Assuntos
Humanos , Masculino , Adulto , Ciclosporina/uso terapêutico , Doxorrubicina/uso terapêutico , Quimioterapia Combinada , Granulomatose com Poliangiite/diagnóstico , Granulomatose com Poliangiite/imunologia , Granulomatose com Poliangiite/patologia , Linfoma Cutâneo de Células T/diagnóstico , Palato Mole/ultraestrutura , Palato/ultraestrutura , Prednisona/uso terapêutico , Vincristina/uso terapêutico , Biópsia , Técnicas Imunoenzimáticas , Sepse/mortalidadeRESUMO
We have compared, at the ultrastructural level, the carbohydrate structure of glycoconjugates of the different types of secretory cells of the human tracheal mucosa (HTM) and the frog palate mucosa (FPM), proposed as a model for studying bacterial adherence to mucus-coated respiratory epithelium. In addition to reactivity with Concanavalin A and Lens cullinaris agglutinin, reactivity of Epon-embedded HTM and FPM secretory granules was studied by transmission electron microscopy using neuraminidase-gold complex and colloidal gold-adsorbed lectins with affinity for sugar residues of human mucins, namely the following: Helix pomatia, Lotus tetragonolobus, Ricinus communis II, Wheat germ and Limax flavus agglutinins. The affinity of HTM and FPM mucous and serous cells for the different colloidal-gold complexes was very similar, however Limax flavus agglutinin labelled only HTM and not FPM secretory granules. The FPM mucous and serous secretory granules were nevertheless intensely labelled by the neuraminidase-gold complex, demonstrating the presence of sialic acid residues. The close ultrastructural and histochemical similarities between HTM and FPM suggest that the FPM may be a valuable model for studying the specific interaction between microbial lectins and mucus glycoproteins in the bacterial adherence phenomenon.
Assuntos
Metabolismo dos Carboidratos , Palato Mole/metabolismo , Traqueia/metabolismo , Animais , Aderência Bacteriana/fisiologia , Coloides , Ouro , Humanos , Lectinas , Microscopia Eletrônica , Mucosa Bucal/metabolismo , Mucosa Bucal/ultraestrutura , Mucosa/metabolismo , Mucosa/microbiologia , Mucosa/ultraestrutura , Neuraminidase , Palato Mole/ultraestrutura , Rana esculenta , Distribuição Tecidual , Traqueia/ultraestruturaRESUMO
Aspectos de superfície celular da mucosa do palato mole de camundongos foram estudados através do microscópio eletrônico de varredura. A superfície mostra células epiteliais que possuem muitas micropregas irregulares e estas se concentram em determinadas áreas do palato mole. As células desta regiäo näo apresentam características superficiais bem diferenciadas. Também, säo observadas apenas poucas características de superfície e as bordas celulares limitantes. A papila gustativa presente na mucosa do palato mole apresenta uma forma arredondada e possui a superfície revestida por células poligonais planas. Estas células possuem os detalhes de superfície celular, as vezes bem evidentes, com a presença de micropregas e forames de diversos tamanhos
Assuntos
Camundongos , Animais , Microscopia Eletrônica de Varredura , Palato Mole/ultraestruturaRESUMO
This paper presents a study of the ultrastructure of the epithelium of the soft plate of the rat. General morphology, ratio and localization of ribosomes, filaments, mitocondria, spaces of stratum basale, stratum spinosum and stratum granulosum, are described. The stratum corneum presents modified desmosomes, no organelles and enlarged intercellular spaces. Cornified cells are constituted of a filament-like material, densely packed in an amorphous matrix.