RESUMO
The aim of this work was to develop and test the in vitro biological activity of nanocapsules loaded with a doxorubicin (DOX) free base dissolved in a core of castor oil shelled by poly(methyl vinyl ether-co-maleic anhydride) conjugated to n-octadecylamine residues. This system was stable and monodisperse, with a hydrodynamic diameter of about 300 nm. These nanocapsules changed the intracellular distribution of DOX, from the nuclei to the cytoplasm, and exhibited higher toxicity towards cancer cells - 4T1 and MCF-7 - and significantly lower toxicity towards normal cells - NIH-3T3 and MCF-10A - in vitro. In conclusion, these nanocapsules are suitable DOX carriers, which remain to be studied in in vivo tumor models.
Assuntos
Neoplasias da Mama/tratamento farmacológico , Doxorrubicina/metabolismo , Portadores de Fármacos/química , Nanocápsulas/química , Animais , Neoplasias da Mama/patologia , Óleo de Rícino , Linhagem Celular , Linhagem Celular Tumoral , Núcleo Celular , Citoplasma , Doxorrubicina/toxicidade , Portadores de Fármacos/normas , Humanos , Células MCF-7 , Camundongos , Células NIH 3T3RESUMO
The aim of this study is to evaluate the thrombogenic behaviour of the low density polyethylene and poly(vinyl chloride) modified by radiation-grafting technique. After copolymerization with acrylic acid by gamma-rays from a 60Co source, BSA was immobilized onto functionalized graft copolymers. The biological interaction between these materials and blood was studies by in vitro methods. The BSA immobilization effectively suppressed the adhesion and activation of platelets when it contacted whole blood.