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The present study evaluated the effect of Ovarian Tissue Cryosystem (OTC) on follicular morphology and density, as well as on stromal cell density of vitrified canine ovarian tissue. Canine ovarian fragments collected from adult female dogs in stages of the random oestrous cycle were fixed (FC, fresh control) or vitrified (VIT) with an OTC device. After vitrification and warming, the fragments were fixed for histological analysis. Overall, the mean percentage of normal pre-antral follicles decreased after vitrification procedure (FC: 74.5% ± 1.6% vs. VIT: 52.05% ± 1.5%). Although the rates of normal primordial (71.1% ± 1.8%) and secondary (0.7% ± 0.4%) follicles vitrified showed a reduction (p < .05), vitrification using OTC showed considerable preservation of follicles, when compared to the fresh control (81.1% ± 1.5% and 2.3% ± 0.6%, respectively). The mean follicular density was maintained after vitrification (FC: 199.65 ± 12.8 vs. VIT: 199.68 ± 10.8), whereas the stromal cell density decreased in the VIT group. Based on the results, we recommend the use of OTC for vitrification of canine ovarian tissue.

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Purpose To compare Fructose-1,6-Bisphosphate (FBP) to Histidine-Tryptophan-Ketoglutarate (HTK) in liver preservation at cold ischemia. Methods Male rats (Sprague-Dawley: 280-340g) divided into three groups (n=7): Control; Fructose-1,6-bisphosphate (FBP); Histidine-Tryptophan-Ketoglutarate (HTK). Animals underwent laparotomy-thoracotomy for perfusion of livers with saline. Livers were removed and deposited into solutions. Mitochondria were isolated to determine State 3 (S3), State 4 (S4), Respiratory Control Ratio (RCR) and Swelling (S). Liver enzymes (AST, ALT, LDH) were determined in solution. At tissue, Malondialdehyde (MDA) and Nitrate (NOx) were determined. All parameters were analyzed at 0.6 and 24 hours of hypothermic preservation. Statistics analysis were made by Mann-Whitney test (p 0.05). Results Regarding ALT, there was a difference between FBP-6h/HTK-6h, lower in HTK. Regarding AST, there was a significant difference between FBP-24h/HTK-24h, lower in FBP. Regarding NOx, there was a difference between 0h and 6h, as well as 0h and 24h for both solutions. Regarding S3, there was a significant difference in 24h compared to Control-0h for both solutions, and a significant difference between FBP-6h/FBP-24h. Regarding S4, there was a difference between Control-0h/HTK-24h and FBP-24h/HTK-24h, higher in HTK. There was a difference between Control-0h/FBP-24h for Swelling, higher in FBP. Conclusion Fructose-1,6-Bisphosphate showed better performance at nitrate and aspartate aminotransferase compared to histidine-tryptophan-ketoglutarate.(AU)

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Purpose: To evaluate the effect of amniotic fluid in liver preservation in organ transplantation, and compare it with standard preservation solutions. Methods: The groups consisted of Group 1: Ringer Lactate (RL) group, Group 2: HTK group, Group 3: UW group, Group 4: AF group. The livers of rats from Group 1, 2, 3, and 4 were perfused and placed into falcon tubes containing RL, HTK, UW, and AF solutions at +4°C, respectively. The tubes were stored for 12 hours in the refrigerator at +4°C. Tissue samples were taken at the 6th and 12th hours for histopathological examinations of the perfused livers, and storage solutions for biochemical analyzes at 6th and 12th hours. Results: AF was shown to maintain organ viability by reducing the number of cells undergoing apoptosis. Histopathological changes such as sinusoidal dilatation, hydropic degeneration, and focal necrosis were found to be similar to the groups in which the standard organ preservation solutions were used. Additionally, the results of INOS, IL-10, and TNF-,which were evaluated immunohistochemically, have been shown to be similar to the UW and HTK groups. Conclusions: AF provided conservation similar to UW and HTK in the 12-hour liver SCS process. The fact that apoptosis values are comparable to standard preservation solutions supports the success of AF in the cold storage of the liver.(AU)

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Um equino macho, com 6 anos de idade, foi atendido no Hospital Veterinário, com carcinoma de células escamosa, após o animal ter sido previamente tratado com quimioterápico 5 Fluorouracil tópico para reduzir a ferida, após o tratamento foi realizado a excisão cirúrgica do tumor, e na lesão resultante do procedimento cirúrgico, foi realizado o curativo com pericárdio bovino conservado em glicerina a 98%, o tratamento durou 30 dias com boa remissão da ferida, apresentando boa contração da ferida, sem produção de secreção. O pericárdio bovino é uma opção barata e de fácil conservação em feridas extensas em equinos.(AU)

A 6-year-old male horse was treated at the Veterinary Hospital with squamous cell carcinoma after the animal had been previously treated with topical Fluorouracil 5 to reduce the wound, after the treatment the tumor was excised surgically, and in the lesion resulting from the surgical procedure, the dressing was performed with 98% glycerin preserved bovine pericardium, the treatment lasted for 30 days with good wound remission, showing good contraction of the wound, without secretion. The bovine pericardium is an inexpensive and easy-care option in extensive wounds on horses.(AU)

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RESUMO

Um equino macho, com 6 anos de idade, foi atendido no Hospital Veterinário, com carcinoma de células escamosa, após o animal ter sido previamente tratado com quimioterápico 5 Fluorouracil tópico para reduzir a ferida, após o tratamento foi realizado a excisão cirúrgica do tumor, e na lesão resultante do procedimento cirúrgico, foi realizado o curativo com pericárdio bovino conservado em glicerina a 98%, o tratamento durou 30 dias com boa remissão da ferida, apresentando boa contração da ferida, sem produção de secreção. O pericárdio bovino é uma opção barata e de fácil conservação em feridas extensas em equinos.

A 6-year-old male horse was treated at the Veterinary Hospital with squamous cell carcinoma after the animal had been previously treated with topical Fluorouracil 5 to reduce the wound, after the treatment the tumor was excised surgically, and in the lesion resulting from the surgical procedure, the dressing was performed with 98% glycerin preserved bovine pericardium, the treatment lasted for 30 days with good wound remission, showing good contraction of the wound, without secretion. The bovine pericardium is an inexpensive and easy-care option in extensive wounds on horses.

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Com o aumento na produção do biodiesel, uma grande quantidade de glicerina bruta foi lançada no mercado derrubando seus preços, e por ter alto impacto ambiental, não pode ser descartada no meio ambiente. Uma das formas de se absorver o excedente de glicerina produzida é a sua utilização na conservação de peças anatômicas. O uso da glicerina purificada nos laboratórios de anatomia animal têm mostrado excelentes resultados quando comparados a outros métodos de conservação. Frente ao exposto, o presente trabalho visa empregar o uso da glicerina bruta na conservação de peças anatômicas em substituição a glicerina purificada, sem perder a qualidade de conservação das peças viabilizando seu uso nos laboratórios de anatomia e reduzindo assim o descarte de produtos nocivos ao meio ambiente. (AU)

With the increase in the production of biodiesel a lot of crude glycerin was launched on the market dropping their prices and have high environmental impact cannot be discarded in the environment. One of the ways to absorb the glycerin produced surplus is its use in the preservation of anatomical parts. The use of purified glycerin in animal anatomy laboratories has shown excellent results when compared to other conservation methods. Based on these this paper aims to employ the use of crude glycerin in the preservation of anatomical parts in the purified glycerin replacement without losing the quality of conservation of parts enabling its use in anatomy laboratories and thus reducing the disposal of products harmful to the environmental preservation. (AU)

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Com o aumento na produção do biodiesel, uma grande quantidade de glicerina bruta foi lançada no mercado derrubando seus preços, e por ter alto impacto ambiental, não pode ser descartada no meio ambiente. Uma das formas de se absorver o excedente de glicerina produzida é a sua utilização na conservação de peças anatômicas. O uso da glicerina purificada nos laboratórios de anatomia animal têm mostrado excelentes resultados quando comparados a outros métodos de conservação. Frente ao exposto, o presente trabalho visa empregar o uso da glicerina bruta na conservação de peças anatômicas em substituição a glicerina purificada, sem perder a qualidade de conservação das peças viabilizando seu uso nos laboratórios de anatomia e reduzindo assim o descarte de produtos nocivos ao meio ambiente.

With the increase in the production of biodiesel a lot of crude glycerin was launched on the market dropping their prices and have high environmental impact cannot be discarded in the environment. One of the ways to absorb the glycerin produced surplus is its use in the preservation of anatomical parts. The use of purified glycerin in animal anatomy laboratories has shown excellent results when compared to other conservation methods. Based on these this paper aims to employ the use of crude glycerin in the preservation of anatomical parts in the purified glycerin replacement without losing the quality of conservation of parts enabling its use in anatomy laboratories and thus reducing the disposal of products harmful to the environmental preservation.

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As membranas biológicas e implantes sintéticos vêm sendo pesquisados com o objetivo de avaliar sua eficiência e ausência de efeitos indesejáveis, (como rejeição e resposta inflamatória exacerbada)na reparação de alterações anatômicas congênitas ou adquiridas. Dentre as membranas biológicas utilizadas, o pericárdio bovino é utilizado devido a algumas características desejáveis, como baixo custo e um nível insignificante de complicações. A glicerina a 98% é bastante utilizada para a preservação do pericárdio bovino devido a suas características químicas e biológicas. Assim sendo, este trabalho tem por objetivo mostrar a diversidade de indicação da utilização do pericárdio bovino conservado em glicerina 98% em 22 pacientes submetidos a procedimentos cirúrgicos em cães. Os resultados obtidos foram satisfatórios, não observando rejeição ao implante.(AU)

The biological membranes and synthetic implants have been studied aiming to evaluate it sefficiency and absence of un desirable effects such as rejection and exacerbated inflammatory response in the repair of congenital or acquired anatomical changes. Among the biological membranes used bovine pericardium is used mainly due to some desirable characteristics such as low cost and negligible level of complications. A 98% glycerin is widely used for the preservation of bovine pericardium because of its chemical and biological characteristics. Thus, this work aims to show the diversity of indication of theuse of bovine pericardium preserved in glycerol 98% in 22 patients undergoing surgical procedures indogs. The results observed is satisfactory and don't see rejection of the implant ar another undesirable complications.(AU)

Las membranas biológicas y los implantes sintéticos han sido estudiados con el objetivo de evaluar su eficacia y falta de efectos indeseables, tales como rechazo y exacerbada respuesta inflamatoria en la reparación de los cambios anatómicos congénitos o adquiridos. Entre las membranas biológicas utilizadas pericardio bovino se utiliza principalmente debido ai bajo costo y las un nivel insignificante de complicaciones. Un glicerina 98% es ampliamente utilizado para la preservación de pericardio bovino para dividir sus características químicas y biológicas. Para tanto, este trabajo pretende mostrar la diversidad de la indicación dei uso de pericardio bovino conservado en glicerol ai 98% en 22 pacientes sometidos a procedimientos quirúrgicos en los perros. Los resultados fureran buenos y no observaron rechazo dei implante.(AU)

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As membranas biológicas e implantes sintéticos vêm sendo pesquisados com o objetivo de avaliar sua eficiência e ausência de efeitos indesejáveis, (como rejeição e resposta inflamatória exacerbada)na reparação de alterações anatômicas congênitas ou adquiridas. Dentre as membranas biológicas utilizadas, o pericárdio bovino é utilizado devido a algumas características desejáveis, como baixo custo e um nível insignificante de complicações. A glicerina a 98% é bastante utilizada para a preservação do pericárdio bovino devido a suas características químicas e biológicas. Assim sendo, este trabalho tem por objetivo mostrar a diversidade de indicação da utilização do pericárdio bovino conservado em glicerina 98% em 22 pacientes submetidos a procedimentos cirúrgicos em cães. Os resultados obtidos foram satisfatórios, não observando rejeição ao implante.

The biological membranes and synthetic implants have been studied aiming to evaluate it sefficiency and absence of un desirable effects such as rejection and exacerbated inflammatory response in the repair of congenital or acquired anatomical changes. Among the biological membranes used bovine pericardium is used mainly due to some desirable characteristics such as low cost and negligible level of complications. A 98% glycerin is widely used for the preservation of bovine pericardium because of its chemical and biological characteristics. Thus, this work aims to show the diversity of indication of theuse of bovine pericardium preserved in glycerol 98% in 22 patients undergoing surgical procedures indogs. The results observed is satisfactory and don't see rejection of the implant ar another undesirable complications.

Las membranas biológicas y los implantes sintéticos han sido estudiados con el objetivo de evaluar su eficacia y falta de efectos indeseables, tales como rechazo y exacerbada respuesta inflamatoria en la reparación de los cambios anatómicos congénitos o adquiridos. Entre las membranas biológicas utilizadas pericardio bovino se utiliza principalmente debido ai bajo costo y las un nivel insignificante de complicaciones. Un glicerina 98% es ampliamente utilizado para la preservación de pericardio bovino para dividir sus características químicas y biológicas. Para tanto, este trabajo pretende mostrar la diversidad de la indicación dei uso de pericardio bovino conservado en glicerol ai 98% en 22 pacientes sometidos a procedimientos quirúrgicos en los perros. Los resultados fureran buenos y no observaron rechazo dei implante.

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In this study we aimed testing the efficiency of a newly developed device for vitrification of ovaries without contact with liquid nitrogen, Ovarian Tissue Cryosystem (OTC). From each ovarian pair, fragments were recovered and immediately fixed for analysis (fresh control) or submitted to vitrification (fragments, hemi-ovary or whole ovary), either or not followed by in vitro culture for two days. Vitrification was performed using the OTC system. The OTC is a cylindrical structure made by stainless steel and composed by three pieces (basis, insert and cover), which can be hermetically closed avoiding contact of the tissue with liquid nitrogen during vitrification. Before and after culture, the ovarian tissue was histologically evaluated. Independently from the size of the ovarian tissue, it was observed a decrease (P<0.05) in the rates of normal preantral follicles when fragments (58.1%), hemi-ovary (54.4%) and whole ovary (54.3%) were vitrified, in comparison with fresh control (68.1%). These data were confirmed by ultrastructural analysis, which showed a great extension of degeneration in follicles vitrified in the whole ovary. Follicular survival after vitrification followed by culture was higher (P<0.05) when ovarian fragments were vitrified (36.1%) than in those enclosed in vitrified hemi-ovary (22.3%) or whole ovary (18.4%). In conclusion, the Ovarian Tissue Cryosystem (OTC) opens a new possibility for successful vitrification of caprine ovarian fragments.

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