RESUMO
OBJECTIVE: Microchimerism has been extensively investigated in autoimmune diseases, which display similarities with graft-vs-host disease. This study was conducted to investigate the presence of microchimerism in minor salivary glands of hematopoietic stem cell transplanted patients, one of the targets of graft-vs-host disease. METHODS: Labial salivary glands biopsy specimens from 11 stem cell transplanted patients were analysed. The samples were grouped in control (five specimens from a female-to-female transplantation) and study group (five glands from male-to-female transplantation). One male transplanted patient was used as a positive control. Fluorescence in situ hybridization with Y-chromosome probe and immunofluorescence with anticytokeratin AE1/AE3 and CD45 were used to identify Y-chromosome positive glandular epithelial cells from allogeneic hematopoietic stem cell transplanted patients. RESULTS: In the study group, all samples were positive to Y-chromosome and cytokeratin AE1/AE3, in agreement with the pattern exhibited by male labial salivary gland. None of the samples from control group were positive to Y-chromosome despite being positive to cytokeratin AE1/AE3. Positivity to CD45 was not relevant. CONCLUSION: Microchimerism in the labial salivary glands of sex-mismatched stem cell transplanted patients is a real phenomenon. Further studies are necessary to elucidate the impact of this phenomenon on the clinical status of stem cell transplanted patients.
Assuntos
Quimerismo/classificação , Transplante de Células-Tronco Hematopoéticas/classificação , Lábio/patologia , Glândulas Salivares Menores/patologia , Adolescente , Adulto , Biópsia , Cromossomos Humanos Y/genética , Células Epiteliais/patologia , Feminino , Imunofluorescência , Doença Enxerto-Hospedeiro/patologia , Humanos , Hibridização in Situ Fluorescente , Queratina-1/análise , Queratina-3/análise , Antígenos Comuns de Leucócito/análise , Masculino , Microscopia Confocal , Pessoa de Meia-Idade , Transplante HomólogoAssuntos
Neoplasias Faciais/diagnóstico , Sarcoma Sinovial/diagnóstico , Neoplasias de Tecidos Moles/diagnóstico , Criança , Fossa Craniana Média/patologia , Diagnóstico Diferencial , Neoplasias Faciais/patologia , Feminino , Seguimentos , Humanos , Queratina-1/análise , Queratina-3/análise , Mucina-1/análise , Proteínas Proto-Oncogênicas c-bcl-2/análise , Rabdomiossarcoma Embrionário/diagnóstico , Sarcoma Sinovial/patologia , Neoplasias de Tecidos Moles/patologia , Vimentina/análiseRESUMO
This case report describes a 10-year-old female patient with an adenomatoid odontogenic tumor developing together with a cystic complex odontoma. This occurrence is considered very unusual. Immunohistochemical detection of cytokeratins AE1/AE3, CK5, CK8, CK10, CK14, CK19 and Ki-67 was performed.