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Among antihyperglycemic drugs used for treating diabetes, α-glucosidase inhibitors generate the least adverse effects. This contribution aimed to evaluate the potential antidiabetic activity of Rumex crispus L. by testing its in vitro α-glucosidase inhibition and in vivo antihyperglycemic effects on rats with streptozotocin (STZ)-induced diabetes. Better inhibition of α-glucosidase was found with the methanol extract versus the n-hexane and dichloromethane extracts. The methanol extract of the flowers (RCFM) was more effective than that of the leaves (RCHM), with an IC50 of 7.3 ± 0.17 µg/mL for RCFM and 112.0 ± 1.23 µg/mL for RCHM. A bioactive fraction (F89s) also showed good α-glucosidase inhibition (IC50 = 3.8 ± 0.11 µg/mL). In a preliminary study, RCHM and RCFM at 150 mg/kg and F89s at 75 mg/kg after 30 days showed a significant effect on hyperglycemia, reducing glucose levels (82.2, 80.1, and 84.1%, respectively), and improved the lipid, renal, and hepatic profiles of the rats, comparable with the effects of metformin and acarbose. According to the results, the activity of R. crispus L. may be mediated by a diminished rate of disaccharide hydrolysis, associated with the inhibition of α-glucosidase. Thus, R. crispus L. holds promise for the development of auxiliary drugs to treat diabetes mellitus.

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O presente estudo investigou a eficácia e o nível ideal de alimentação de folhas de Rumex nervosus (RNL) em frangos de corte infectados e não infectados com Eimeria tenella . Em um planejamento fatorial de 2 (desafio coccidiano ou não) 5 (tratamentos alimentares), pintos de corte de 250 dias de idade foram distribuídos aleatoriamente em um dos dez grupos e criados por 28 dias. Foram investigadas medidas intestinais, características de carcaça, índices bioquímicos séricos, leucogramas, desempenho e indicadores anticoccidianos. Todos os parâmetros selecionados não foram significativos ( p>0,05) alterado pelas interações entre dieta experimental e desafio aos 7 dias pós-infecção, com exceção dos níveis séricos de albumina e globulina, bem como do índice anticoccidiano. Os frangos infectados apresentaram menor indicador de desempenho, menor eficiência de produção, baixos índices anticoccidianos, moela mais pesada, intestino delgado mais longo e mais pesado, ceco mais curto e maior proporção de eosinófilos em comparação com frangos não infectados. O RNL apresentou eficácia anticoccidiana marcante na dose de 5 g. O presente estudo descobriu que o pó da folha de Rumex nervosus apresentou eficácia preventiva anticoccidiana de leve a acentuada, dependente da dose, em frangos de corte. No entanto, mais estudos são necessários para compreender completamente os mecanismos anticoccidianos.(AU)

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A língua-de-vaca é uma planta daninha perene, que infesta principalmente pastagens, lavouras anuais e perenes na região Sul do Brasil. Por ser uma planta perene, o controle se torna mais difícil devido ao rebrote. O trabalho teve como objetivo acompanhar a fenologia e avaliar as características de crescimento e desenvolvimento de plantas de língua-de-vaca em ambiente não competitivo. O experimento foi conduzido em casa de vegetação, após as sementes terem germinado em câmara BOD, as plântulas foram transplantadas para vasos preenchidos com Cambissolo Háplico. Foram realizadas análises destrutivas aos 0, 33, 94, 124, 156, 186 e 215 dias após o transplantio (DAT). As plantas foram coletadas separando a parte aérea do sistema radicular e da inflorescência, cada parte da planta foi levada à estufa de secagem por circulação forçada de ar para posterior determinação da massa seca. Observou-se que as plantas de língua-de-vaca apresentam crescimento inicial bastante lento. A distribuição da massa seca total foi de 56,8% do total acumulado no sistema radicular, seguido pela inflorescência (31,6%) e parte aérea (11,6%). O número médio de sementes produzidas foi de 3.406 (± 389) por plantas.

Broad-leaved dock is a perennial weed, which infests mainly pastures, annual crops and perennials in the Southern of Brazil. Because it is a perennial plant, control becomes more difficult due to regrowth. The objective of this study was to evaluate the growth and development characteristics of cow-tongue plants in a non-competitive environment. The objective of this work was to monitor phenology and to evaluate the growth and development characteristics of broad-leaved dock plants in the non-competitive environment. The experiment was conducted in a greenhouse, after the seeds germinated in the BOD chamber, the seedlings were transplanted to pots filled with Cambisol Haplic. Destructive analyzes were performed at 0, 33, 94, 124, 156, 186 and 215 days after transplanting (DAT). The plants were collected separating the aerial part of the root system and the inflorescence, each part of the plant was taken to the drying oven by forced circulation of air for later determination of the dry mass. It was observed that the broad-leaved dock plants present initial growth rather slow. The total dry mass distribution was 56.8% of the total accumulated in the root system, followed by inflorescence (31.6%) and shoot (11.6%). The average number of seeds produced was 3,406 (± 389) per plant.ien

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A língua-de-vaca é uma planta daninha perene, que infesta principalmente pastagens, lavouras anuais e perenes na região Sul do Brasil. Por ser uma planta perene, o controle se torna mais difícil devido ao rebrote. O trabalho teve como objetivo acompanhar a fenologia e avaliar as características de crescimento e desenvolvimento de plantas de língua-de-vaca em ambiente não competitivo. O experimento foi conduzido em casa de vegetação, após as sementes terem germinado em câmara BOD, as plântulas foram transplantadas para vasos preenchidos com Cambissolo Háplico. Foram realizadas análises destrutivas aos 0, 33, 94, 124, 156, 186 e 215 dias após o transplantio (DAT). As plantas foram coletadas separando a parte aérea do sistema radicular e da inflorescência, cada parte da planta foi levada à estufa de secagem por circulação forçada de ar para posterior determinação da massa seca. Observou-se que as plantas de língua-de-vaca apresentam crescimento inicial bastante lento. A distribuição da massa seca total foi de 56,8% do total acumulado no sistema radicular, seguido pela inflorescência (31,6%) e parte aérea (11,6%). O número médio de sementes produzidas foi de 3.406 (± 389) por plantas.(AU)

Broad-leaved dock is a perennial weed, which infests mainly pastures, annual crops and perennials in the Southern of Brazil. Because it is a perennial plant, control becomes more difficult due to regrowth. The objective of this study was to evaluate the growth and development characteristics of cow-tongue plants in a non-competitive environment. The objective of this work was to monitor phenology and to evaluate the growth and development characteristics of broad-leaved dock plants in the non-competitive environment. The experiment was conducted in a greenhouse, after the seeds germinated in the BOD chamber, the seedlings were transplanted to pots filled with Cambisol Haplic. Destructive analyzes were performed at 0, 33, 94, 124, 156, 186 and 215 days after transplanting (DAT). The plants were collected separating the aerial part of the root system and the inflorescence, each part of the plant was taken to the drying oven by forced circulation of air for later determination of the dry mass. It was observed that the broad-leaved dock plants present initial growth rather slow. The total dry mass distribution was 56.8% of the total accumulated in the root system, followed by inflorescence (31.6%) and shoot (11.6%). The average number of seeds produced was 3,406 (± 389) per plant.(AU)ien

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Due to increased number of herbicide resistant weeds, it is needed to explore the allelopathic potential of plants as an alternative. The research was conducted to investigate allelopathic effects of Carica papaya L. leaf powder and aqueous extract on seeds as well as pre-germinated seeds of Avena fatua L., Helianthus annuus L., Rumex dentatus L., Zea mays L. and Triticum aestivum L. on filter paper and soil in Weed Management Program Laboratory, Department of Plant and Environmental Protection at PARC Institute of Advanced Studies in Agriculture, National Agriculture Research Centre, Islamabad, Pakistan. Germination percentage (%), radicle length (cm) and plumule length (cm) were parameters observed for Plant leaf powder bioassay and Aqueous extract method. Most significant growth inhibition was observed in A. fatua seedlings in filter paper method. A. fatua radicle length was reduced by C. papaya aqueous extract (80%) and leaf powder (89%) bioassays. Plumule length was reduced under the influence of aqueous extract (57-73%) and powdered material (59-77%). The inhibitory effects on other test species were in sequence of H. annuus followed by Z. mays and R. dentatus. The aqueous extract showed non-significant effect on wheat seed germination, radicle and plumule growth. It is suggested that C. papaya aqueous extract can be used as source of weed management in wheat crop.(AU)

Devido ao aumento do número de ervas daninhas resistentes aos herbicidas, é necessário explorar o potencial alelopático das plantas como uma alternativa. A pesquisa foi conduzida com o objetivo de investigar os efeitos alelopáticos do pó foliar de Carica papaya e do extrato aquoso das sementes, bem como das sementes pré-germinadas de Avena fatua, Helianthus annuus, Rumex dentatus, Zea mays e Triticum aestivum em papel de filtro e solo no Laboratório do Programa de Manejo de Ervas Daninhas, Departamento de Plantas e Proteção Ambiental do Instituto PARC de Estudos Avançados em Agricultura, Centro Nacional de Pesquisa Agrícola, Islamabad, Paquistão. A porcentagem de germinação (%), o comprimento radicular e o comprimento da plúmula (cm) foram os parâmetros observados para o 'Bioensaio de Pó de Folha de Planta' e o 'Método de Extração Aquoso'. A maior inibição do crescimento foi observada em mudas de A. fatua no método de papel de filtro. O comprimento radicular de A. fatua foi reduzido com os extratos aquosos de C. papaya (80%) e pó de folhas (89%). O comprimento das plúmulas foi reduzido sob a influência do extrato aquoso (57-73%) e material em pó (59-77%). Os efeitos inibitórios em outras espécies-teste foram na sequência de H. annuus seguido por Z. mays e R. dentatus. O extrato aquoso apresentou efeito não significativo na germinação das sementes de trigo, nos crescimentos radiculares e das plúmulas. Sugere-se que o extrato aquoso de C. papaya pode ser utilizado como fonte de manejo de plantas daninhas na cultura do trigo.(AU)

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The revival of non-conventional edible plants enables important gains in nutritional, social, cultural, economic, and environmental points of view. Among them, sorrel ­ Rumex acetosa L. ­ has been deemed pleasant by consumers due to its acid taste. As we do not have a lot of phytotechnical andnutritional information about this plant, the objective was to assess the influence of different spacing sets and cattle manure dosages on their physical-chemical characteristics and bioactive compounds content with antioxidant action. Randomized blocks with four repetitions was chosen as experimental design, using ascheme of subdivided plots, with five dosages of weather-beaten cattle manure in the plots and two sets of spacing in the subplots. Data was submitted to a variance analysis with two factors (two-way ANOVA). It was not verified any significant effect (P>0.05) in the interaction Dosage x Spacing. Fertilization dosages and spacing sets did not have any effect on color and total soluble solids average contents, humidity, titratable acidity, and phenolic compounds. Increasing dosages of organic fertilizer, up to 75 kg ha-1, reduced the plant's pH; above that amount, pH rose. Presence of anthocyanins and vitamin C was not detected. The total carotenoids average content was not influenced by the manure factor, and the plant presented greater total carotenoids content in the smallest spacing. Antioxidant activity [1.0 mg mL-1], although inferior to the standards that were used (BHT, vitamin C and quercetin), was quite representative. A conclusion was reached that fertilizing with weather-beaten cattle manure had an effect only on pH, and cropping spacing had an influence only on carotenoids content. Other phytochemicals and physical-chemical characteristics were not influenced in sorrel plants with different levels of fertilization and sets of spacing.

O resgate das plantas comestíveis não convencionais possibilita ganhos importantes do ponto de vista nutricional, social, cultural, econômico e ambiental. Dentre estas, a azedinha, Rumex acetosa L. tem agradado o consumidor por seu sabor ácido. Como são poucas as informações fitotécnicas e nutricionais sobre esta planta objetivou-se avaliar a influência de diferentes espaçamentos e doses de esterco bovino em suas características físico-químicas e no teor de compostos bioativos com função antioxidante. O delineamento experimental foi em blocos casualizados com quatro repetições, no esquema de parcelas subdivididas, sendo cinco doses de esterco curtido de gado nas parcelas e dois espaçamentos nas subparcelas. Os dados foram submetidos à análise de variância com dois fatores (two-way ANOVA). Não foi constatado efeito significativo (P>0,05) da interação Dose x Espaçamento. As doses de adubação e espaçamentos não afetaram a cor e os teores médios de sólidos solúveis totais, umidade, acidez titulável e compostos fenólicos. Doses crescentes de adubo orgânico, até 75 kg ha-1, reduziram o pH da planta, acima deste valor o pH elevou-se. Não foi detectada a presença de antocianinas e vitamina C. O teor médio de carotenoides totais não foi influenciado pelo fator esterco e no menor espaçamento a planta apresentou maior teor de carotenoides totais. A atividade antioxidante [1,0 mg mL-1], apesar de inferior aos padrões utilizados (BHT, vitamina C e quercetina), foi bastante representativa. Concluiu-se que a adubação com esterco de gado curtido afetou apenas o pH e que o espaçamento de cultivo exerceu influência apenas teor de carotenoides. Os demais fitoquímicos e características físico-químicas não foram influenciadas em plantas de azedinha com diferentes níveis de adubação e de espaçamento

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Abstract Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer. Among these fungi, Aspergillus sp. showed the most significant enhancement on bioactive components accumulation in RGT seedlings. When inoculated Aspergillus sp. spores into media of RGT seedlings that had taken root for 20 d, and made spore concentration in co-culture medium was 1 × 104 mL-1, after co-cultured for 12 d, the yield of chrysophaein, resveratrol, chrysophanol, emodin and physcion were 3.52-, 3.70-, 3.60-, 4.25-, 3.85-fold of the control group. The extreme value of musizin yield was 0.289 mg, which was not detected in the control groups. The results indicated that co-culture with endophytic fungi could significantly enhance bioactive secondary metabolites production of RGT seedlings.

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The objective of this study was to characterize the nutritional compounds of interest present in vegetables known as non-conventional, in Brazil. The following evaluations were carried out: antioxidant activity, phenolic compounds, vitamin C, calories, carbohydrates, humidity, lipids, proteins, fiber, acidity and quantification of minerals (P, K, Ca, Mg, S, Cu, Fe, Mn, Zn and B). The species studied were Amaranthus hybridus L., Amaranthus viridis L., Basella alba L., Eryngium campestre L., Hibiscus sabdariffa L., Lactuca canadensis L., Rumex acetosa L., Stachys byzantina K. Koch, Tropaeolum majus L. and Xanthosoma sagittifolium L. Representative samples of plant structures of interest were harvested from each species suitable for human consumption such as leaves, flowers and flower buds. The results were submitted to multivariate analysis - principal components analysis (PCA). All the species present nutritional compounds of interest in different levels among the evaluated structures.

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Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer. Among these fungi, Aspergillus sp. showed the most significant enhancement on bioactive components accumulation in RGT seedlings. When inoculated Aspergillus sp. spores into media of RGT seedlings that had taken root for 20d, and made spore concentration in co-culture medium was 1×104mL-1, after co-cultured for 12d, the yield of chrysophaein, resveratrol, chrysophanol, emodin and physcion were 3.52-, 3.70-, 3.60-, 4.25-, 3.85-fold of the control group. The extreme value of musizin yield was 0.289mg, which was not detected in the control groups. The results indicated that co-culture with endophytic fungi could significantly enhance bioactive secondary metabolites production of RGT seedlings.

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BACKGROUND: Cancer, being the foremost challenge of the modern era and the focus of world-class investigators, gargantuan research is in progress worldwide to explore novel therapeutic for its management. The exploitation of natural sources has been proven to be an excellent approach to treat or minify the excessive angiogenesis and proliferation of cells. Similarly, based the ethnomedicinal uses and literature survey, the current study is designed to explore the anti-tumor and anti-angiogenic potentials of Rumex hastatus. Anti-tumor and anti-angiogenic activities were carried out using potato-disc model and chorioallantoic membrane (CAM) assay respectively. Moreover, R. hastatus was also assessed for antibacterial activity against Agrobacterium tumefaciens (tumor causing bacterial strain). The positive controls used in anti-tumor, anti-angiogenic and antibacterial activities were vincristine sulphate, dexamethasone and cefotaxime respectively. RESULTS: The crude saponins (Rh.Sp), methanolic extract (Rh.Cr) and other solvent extracts like n-hexane (Rh.Hex), chloroform (Rh.Chf), ethylacetate (Rh.EtAc) and aqueous fraction (Rh.Aq) exhibited notable anti-tumor and anti-angiogenic activities. In potato tumor assay, the chloroform and saponin fractions were observed to be the most effective showing 86.7 and 93.3 % tumor inhibition at 1000 µg/ml with IC50 values 31.6 and 18.1 µg/ml respectively. Similarly, these two samples i.e., chloroform and saponins also excelled among the entire test samples in anti-angiogenic evaluation exhibiting 81.6 % (IC50 = 17.9 µg/ml) and 78.9 % (IC50 = 64.9 µg/ml) at 1000 µg/ml respectively. In contrast, the antibacterial investigations revealed a negligible potential against A. tumefaciens. CONCLUSION: Based on our results we can claim that R. hastatus possesses both anti-tumor and anti-angiogenic potentials. In all of the solvent fractions, Rh.Chf and Rh.Sp were most effective against tumor and angiogenesis while having negligible activity against A. tumefaciens. It can be concluded that Rh.Chf and Rh.Sp might be potential targets in the isolation of natural product having anti-neoplastic action.

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