RESUMO
One of the challenges in augmentative biological control programs is the definition of releasing strategy for natural enemies, especially when macro-organisms are involved. Important information about the density of insects to be released and frequency of releases usually requires a great number of experiments, which implies time and space that are not always readily available. In order to provide science-based responses for these questions, computational models offer an in silico option to simulate different biocontrol agent releasing scenarios. This allows decision-makers to focus their efforts to more feasible options. The major insect pest in sugarcane crops is the sugarcane borer Diatraea saccharalis, which can be managed using the egg parasitoid Trichogramma galloi. The current strategy consists in releasing 50,000 insects per hectare for each release, in three weekly releases. Here, we present a simulation model to check whether this releasing strategy is optimal against the sugarcane borer. A sensitive analysis revealed that the population of the pest is more affected by the number of releases rather than by the density of parasitoids released. Only the number of releases demonstrated an ability to drive the population curve of the pest towards a negative growth. For example, releasing a total of 600,000 insects per hectare in three releases led to a lower pest control efficacy that releasing only 250,000 insects per hectare in five releases. A higher number of releases covers a wider range of time, increasing the likelihood of releasing parasitoids at the correct time given that the egg stage is short. Based on these results, it is suggested that, if modifications to the releasing strategy are desired, increasing the number of releases from 3 to 5 at weekly intervals is most likely preferable.
Assuntos
Simulação por Computador , Controle Biológico de Vetores , Saccharum , Animais , Saccharum/parasitologia , Controle Biológico de Vetores/métodos , Mariposas/parasitologia , Himenópteros/fisiologia , Lepidópteros/fisiologia , Lepidópteros/parasitologiaRESUMO
Pest insects are one of the major factors affecting the productivity of sugarcane, and especially those associated with the soil, which damage the crop if not controlled. There is little information on the insect associated with the soil in the sugarcane crop in the state of Mato Grosso do Sul, Brazil. This study evaluated the occurrence, abundance and population variation of soil-associated insects in sugarcane crops in four counties in the state of Mato Grosso do Sul during a period from September 2009 to August 2010. For the capture of insects, deep in the soil trenches were (50 x 50 x 30 width x length x depth). It was observed that county of Maracaju has a high incidence of soil insects in sugarcane crop. Specimens of the family Scarabaeidae are abundant in sugarcane fields of the state of Mato Grosso do Sul, being Liogenys suturalis the predominant species. Soil insects of the Chrysomelidae family were found with greater abundance in Dourados and Naviraí while Noctuidae, represented only by the Hyponeuma taltula, occurred mainly in Dourados. Insects of the family Termitidae occur in sugarcane fields in the counties of Maracaju and Naviraí but not in Nova Alvorada do Sul and Dourados. Sphenophorus levis and Metamasius hemipterus were alsoreported in the sugarcane fields from Maracaju, being the occurrence these species the first record in the Mato Grosso do Sul State. The determined occurrence of different soil insects in the sugarcane fields may help in their management in the four counties studied.
Os insetos-praga são um dos principais fatores que afetam a produtividade da cana-de-açúcar e, principalmente, aqueles associados ao solo, que prejudicam a cultura se não forem controlados. Existem poucas informações sobre a fauna de insetos associada ao solo na cultura da cana-de-açúcar no estado de Mato Grosso do Sul (MS), Brasil. O objetivo deste estudo foi avaliar a ocorrência, abundância e variação populacional de insetos associados ao solo em lavouras de cana-de-açúcar em quatro municípios do estado de Mato Grosso do Sul durante o período de setembro de 2009 a agosto de 2010. Para a captura de insetos, foram feitas trincheiras (50 cm x 50 cm x 30 cm - largura x comprimento x profundidade) no solo. Observou-se que o município de Maracaju apresenta alta incidência de insetos de solo na cultura da cana-de-açúcar. Exemplares da família Scarabaeidae são abundantes nos canaviais do MS, sendo Liogenys suturalis a espécie predominante. Insetos de solo da família Chrysomelidae foram encontrados com maior abundância em Dourados e Naviraí enquanto Noctuidae, representado apenas por Hyponeuma taltula, ocorreu principalmente em Dourados. Insetos da família Termitidae ocorrem em canaviais nos municípios de Maracaju e Naviraí, mas não em Nova Alvorada do Sul e Dourados. Sphenophorus levis e Metamasius hemipterus (Coleoptera: Curculionidae) também foram encontrados nos canaviais de Maracaju, sendo a ocorrência dessas espécies o primeiro registro no MS. A ocorrência determinada de diferentes insetos de solo nos canaviais auxiliará o manejo nos quatro municípios estudados.
Assuntos
Análise do Solo , Pragas da Agricultura , Saccharum/parasitologia , InsetosRESUMO
ABSTRACT: Sugarcane borer Diatraea saccharalis (F.) is the primary sugarcane pest in Brazil. To estimate the relationship between larvae in sugarcane stalks and captures of male adults of D. saccharalis, we collected samples weekly: (1) adults with one delta trap with three virgin females and three female pupae and (2) larvae in 120 stalks per plot of 12.6 hectares (355 × 355 m). The study was conducted in two sites with five plots each, in the municipalities of Nova Ponte and Tupaciguara, Minas Gerais State, Brazil, from July 2016 to May 2017. Relationships between (1) males trapped per week and the number of larvae outside of stalks (LOS) were estimated and (2) we evaluated climate variables, namely average temperature, average relative air humidity, hours with relative air humidity below 30 %, rainfall and number of rainy days, and adults and larvae of D. saccharalis. We obtained generalized linear models for LOS in autumn and for larvae inside the stalks (LIS) in spring and autumn and trapped males in both sites. A significant and direct relationship between LIS and males trapped allows predicting larvae density based on captures of males. In addition, plant damage can be estimated based on accumulated captures of males. There was a negative relationship between hours of air humidity < 30 % and larvae outside of stalks. Densities of LIS can be estimated from male captures and by the humidity variables in the trapping week. Nevertheless, the models require validation in the field.
Assuntos
Pragas da Agricultura , Precipitação Atmosférica , Saccharum/parasitologia , Larva/crescimento & desenvolvimento , Modelos Lineares , UmidadeRESUMO
Sugarcane-associated nematodes (Saccharum spp.) can reduce productivity up to 50%. Through the survey, it was possible to identify the main nematodes that occur in a certain region as a tool for designing the best management and control strategies. The aim of this study was to characterize the population of nematodes associated with sugarcane in the North Central, North Pioneiro and Northwest mesoregion of the state of Paraná, Brazil, quantify the nematode genera associated with the crop and identify the species of Pratylenchus and Meloidogyne. A total amount of 89 soil and root composite samples were collected in nine municipalities. Nematodes were extracted and counted in a Peters counting chamber under an optical light microscope. Morphological description followed identification keys. Pratylenchus spp. were identified by morphological characteristics; Meloidogyne spp. were identified by morphological characteristics and isoenzyme electrophoresis. Twelve genera of nematodes associated with sugarcane were identified: Pratylenchus, Meloidogyne, Helicotylenchus, Xiphinema, Mesocriconema, Trichodorus, Aphelenchus, Hoplolaimus, Tylenchus, Tylenchorhynchus, Ditylenchus, and Paratrichodorus. The genera Pratylenchus and Meloidogyne were found with the highest frequencies in the roots. Among the species of Pratylenchus, P. zeae and P. brachyurus were found, with P. zeae being the most frequent. Among the Meloidogyne species, only M. javanica was found. These results are essential to aid decision making in the management of phytonematodes, mainly in the development of new control strategies and in directing genetic breeding programs for development of sugarcane cultivars for the Paraná state.
Assuntos
Tylenchoidea , Tylenchida , Saccharum/parasitologia , Nematoides/classificaçãoRESUMO
Plectris aliena Chapin, 1934, a white grub that promotes serious damage to a variety of crops in North America and Australia, has been reported for the first time in Brazil. Larvae and adults of P. aliena were recorded in sugarcane crops in the state of Mato Grosso do Sul, sampled by trenches in the soil with light traps, respectively, during 2012 and 2013. Root consumption was also determined for the first, second and third instar larvae. The species is univoltine; adults were found mainly in Aug and the peak of third instar larval density was in Apr in all fields sampled. Pupae and adults of P. aliena were observed in the soil profile at depths of up to 60 and 80 cm, respectively. Larvae of P. aliena of the second and third instar consumed the primary and secondary roots of sugarcane, causing serious damage. Third instar larvae consumed 916 mg of root fresh matter, representing 72 % of the sugarcane root system. These results assist in furthering the understanding of its larval dynamics in the soil and provide support for Integrated Pest Management.
Assuntos
Animais , Besouros/parasitologia , Distribuição Temporal , Larva/crescimento & desenvolvimento , Saccharum/parasitologiaRESUMO
Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae), the sugarcane spittlebug, is one of the most important pests of this crop in Brazil. The magnitude of its damage depends on the pest population, the plants size when they are infested and the cultivar tolerance. The knowledge about those parameters allows the elaboration of the spittlebug management matrix, in which small plants and less tolerant varieties fields are prioritized for sampling and controlling in relation to developed plants and tolerant varieties fields. Therefore, this study aimed to evaluate the response of sugarcane genotypes to M. fimbriolata, so that they can be correctly allocated in the management matrix. Two experiments were carried out under laboratory conditions using a randomized block design with treatments in a factorial arrangement of 2×11 (experiment 1) and 2×21 (experiment 2), with six (experiment 1) or five replicates (experiment 2). The first factor included two levels of infestation (infested and noninfested plants with spittlebugs), while the second consisted of the cultivars. Cultivars IACSP01-5503, CTC 9004 and RB925211 were considered tolerant to spittlebug, they did not show significant reductions in aboveground biomass, despite showing symptoms of pest attack, as leaves yellowing, while CV6654 and IACSP01-3127 were the least tolerant cultivars, showing the highest reduction in aboveground biomass due to M. fimbriolata infestation. Thus, CV6654 and IACSP01-3127 should be prioritized for sampling and control over the others. On average, spittlebug infestations caused 30.9 % of reduction of aboveground biomass.
A cigarrinha das raízes, Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae), é uma das mais importantes pragas da cana-de-açúcar no Brasil. A grandeza de seus danos depende da população, do tamanho da planta ao sofrer o ataque e da tolerância da cultivar. O conhecimento sobre esses parâmetros permite a elaboração da matriz de manejo de cigarrinha das raízes, na qual canaviais de plantas pouco desenvolvidas e cultivares não tolerantes são priorizados para amostragem e controle em relação a canaviais de plantas mais desenvolvidas e variedades tolerantes. Portanto, o objetivo do presente trabalho foi avaliar a reação de cultivares de cana-de-açúcar à M. fimbriolata, para que elas possam ser adequadamente locadas na matriz de manejo. Dois experimentos foram conduzidos em condições de laboratório, usando o delineamento de blocos casualizados com seis (experimento 1) ou cinco (experimento 2) repetições e tratamentos em arranjo fatorial 2×11 (experimento 1) e 2×21 (experimento 2). O primeiro fator foi representado pelos dois níveis de infestação de cigarrinha (infestado e não infestado), enquanto o segundo, pelas cultivares em estudo. As cultivares IACSP01-5503, CTC 9004 e RB925211 foram consideradas tolerantes a M. fimbriolata, visto que a praga não causou redução significativa da massa verde da parte aérea, apesar de ter provocado alguns sintomas de ataque, como amarelecimento de folhas, enquanto CV6654 e IACSP01-3127 foram as cultivares menos tolerantes, pois mostraram as mais altas reduções na massa verde da parte aérea das plantas. Assim, CV6654 e IACSP01-3127 deveriam ser priorizadas na amostragem e controle em relação às demais. Na média, a cigarrinha das raízes provocou 30,9 % de redução na massa verde da parte aérea das plantas.
Assuntos
Controle de Pragas/métodos , Saccharum/parasitologia , Defesa das Plantas contra Herbivoria , Hemípteros/patogenicidadeRESUMO
Seasonal temperature and precipitation patterns on a global scale are main factors to which insects and plants adapt through natural selection, although periodic outbreaks in insect populations may occur in areas where they had not been previously reported, a phenomenon considered as a consequence of global warming. In this study, we estimate the distribution of sugarcane borers, Diatraea spp., under different climate scenarios (rcp26, rcp45, rcp60 and rcp85.) Insects were collected weekly in four sugarcane fields from four different towns in the department of Caldas (Colombia) during 2017, and also in several sugarcane fields in the Cauca River Valley (CRV) between 2010 and 2017. The influence of climatic variables on different agro-ecological zones of the CRV sugarcane fields was defined by climatic data between 2010 and 2017 (maximum and minimum daily temperatures, and accumulated precipitation). The estimate of an optimal niche for Diatraea spp. includes temperatures between 20°C and 23°C, accumulated annual rainfall between 1200 and 1500 mm, dry months with precipitations below 50 mm, slopes of less than 0.05 degrees, crop heterogeneity with an index of 0.2 and primary production values of 1.0. Data suggests Diatraea population is considerably influenced by adverse climate change effects, under the premise of an increase in local and global temperatures, reducing its population niches as well as the number of individuals.
Assuntos
Mariposas , Animais , Mudança Climática , Colômbia , Aquecimento Global , Mariposas/fisiologia , Dinâmica Populacional , Chuva , Saccharum/parasitologia , Estações do AnoRESUMO
Diatraea saccharalis constitutes a threat to the sugarcane productivity, and obtaining borer tolerant cultivars is an alternative method of control. Although there are studies about the relationship between the interaction of D. saccharalis with sugarcane, little is known about the molecular and genomic basis of defense mechanisms that confer tolerance to sugarcane cultivars. Here, we analyzed the transcriptional profile of two sugarcane cultivars in response to borer attack, RB867515 and SP80-3280, which are considered tolerant and sensitive to the borer attack, respectively. A sugarcane genome and transcriptome were used for read mapping. Differentially expressed transcripts and genes were identified and termed to as DETs and DEGs, according to the sugarcane database adopted. A total of 745 DETs and 416 DEGs were identified (log2|ratio| > 0.81; FDR corrected P value ≤ 0.01) after borer infestation. Following annotation of up- and down-regulated DETs and DEGs by similarity searches, the sugarcane cultivars demonstrated an up-regulation of jasmonic acid (JA), ethylene (ET), and defense protein genes, as well as a down-regulation of pathways involved in photosynthesis and energy metabolism. The expression analysis also highlighted that RB867515 cultivar is possibly more transcriptionally activated after 12 h from infestation than SP80-3280, which could imply in quicker responses by probably triggering more defense-related genes and mediating metabolic pathways to cope with borer attack.
Assuntos
Grão Comestível/genética , Lepidópteros/metabolismo , Saccharum/genética , Transcrição Gênica , Animais , Ciclopentanos/metabolismo , Grão Comestível/metabolismo , Grão Comestível/parasitologia , Larva/genética , Larva/parasitologia , Lepidópteros/patogenicidade , Oxilipinas/metabolismo , Saccharum/parasitologiaRESUMO
The giant sugarcane borer Telchin licus (Drury, 1773) (Lepidoptera: Castniidae) is a day-flying moth pest of sugarcane, pineapples and bananas. To better understand the chemical communication in this species, we examined the morphology of its olfactory system and the chemical composition of its body parts. The ventral surface of the clubbed antennae of T. licus has six morphological types of sensilla: sensilla trichodea, basiconica, chaetica, squamiforma, coeloconica, and auricillica. The telescopic ovipositor shows no evidence of a sexual gland, or female-specific compounds. On the other hand, the midleg basitarsus of males releases (E,Z)-2,13-octadecadienol and (Z,E)-2,13-octadecadienol, which are electroantennographically active in both sexes. These compounds are known female sex pheromones in the Sesiidae family and are male-specific compounds in another castniid moth, although further investigations are necessary to elucidate their ecological role in the Castniidae family.
Assuntos
Mariposas/anatomia & histologia , Mariposas/fisiologia , Animais , Antenas de Artrópodes/anatomia & histologia , Antenas de Artrópodes/química , Antenas de Artrópodes/fisiologia , Antenas de Artrópodes/ultraestrutura , Feminino , Masculino , Mariposas/química , Mariposas/ultraestrutura , Oviposição , Saccharum/parasitologia , Atrativos Sexuais/análise , Atrativos Sexuais/metabolismoRESUMO
INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS: Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR). RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food. CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples.