RESUMO
RATIONALE: Alcoholics are more likely to have infections, mainly in the respiratory system. Alcohol seems to inhibit the immune system. Despite the extensive literature related to alcoholism, data related to the immune system are still not conclusive. OBJECTIVE: The purpose of this study was to verify the influence of acute alcohol intake on colloid distribution in the organs of the mononuclear phagocyte system. METHODS AND RESULTS: Thirteen male Swiss mice were divided into two groups: Group 1 (n = 5) - control, and Group 2 (n = 8) - animals that received 0.5 ml ethanol 50%, 30 minutes before the experiment. Colloidal sulphur labeled with 99mTc was used to evaluate colloid distribution in the liver, spleen and lungs. Colloid clearance was assessed as well. A gamma camera was used to measure the radioactivity of these organs and of a blood clot. No difference was found in the presence of colloid in the organs of both groups. The liver showed the highest phagocytic intake, followed by the spleen and lungs (p = 0.021 for Group 1 and p = 0.003 for Group 2). A minimum amount of radiation remained in the blood of both groups. DISCUSSION: According to the experiential conditions of this work, acute ingestion of alcohol did not interfere with the phagocytic function of the mononuclear phagocyte system in mice.
Assuntos
Alcoolismo/imunologia , Alcoolismo/fisiopatologia , Sistema Fagocitário Mononuclear/imunologia , Animais , Fígado/imunologia , Fígado/fisiologia , Pulmão/imunologia , Pulmão/fisiologia , Masculino , Camundongos , Sistema Fagocitário Mononuclear/fisiologia , Baço/imunologia , Baço/fisiologiaRESUMO
O presente trabalho descreve um método para marcaçäo de Salmonella typhymurium com iodo-131, útil para avaliar tanto os aspectos morfológicos como funcionais do sistema retículo-endotelial. Uma suspensäo contendo 2 x 10**9 bactérias por ml foi marcada con Na131I livre de carregador e de redutor, resultando en um rendimento de marcaçäo de 46,5 ñ 3% e 3,5 ñ 1,3% de iodeto-131 livre. Estudou-se a biodistribuiçäo das bactérias marcadas em ratos, obtendo-se imagens de corpo total aos 15 e 30 minutos após injeçäo em uma câmara de cintilaçäo de campo de visäo amplo, equipada com um colimador "pinhole". As imagens mostraram captaçäo das bactérias pelo fígado e pelo baço normal ou transplantado, dos animais estudados. Os cortes autoradiográficos de baço e fígado mostraram bactérias marcadas dentro das células do sistema retículo-endotelial
Assuntos
Animais , Ratos , Sistema Fagocitário Mononuclear/fisiologia , Fagocitose , Radioisótopos do Iodo , Salmonella typhimurium , Autorradiografia , Fígado , Ratos EndogâmicosRESUMO
The present study describes a method for labeling Salmonella typhymurium with iodine-131 to evaluate both the morphological and the functional characteristics of the reticulo-endothelial system. A suspension containing 2 x 10(9) bacteria per ml was labeled with carrier-free Na131I without reductor, with a labeling yield of 46.5 +/- 3% and 3.5 +/- 1.3% of free Iodine-131. The biodistribution of the labeled bacteria in rats was studied with a large-field-of-view scintillation camera equiped with a pinhole collimator. Whole body images were obtained 15 and 30 minutes after intravenous injection of the labeled microorganisms. Images showed accumulation of bacteria in the liver and both normal and transplanted spleens of the animals. Autoradiographs of liver and spleen demonstrated labeled bacteria within the cells of the reticulo-endothelial system. The method described is easy to perform, has a good labeling yield and allows the functional evaluation of the reticulo-monophagocytic system, including transplanted spleens.
Assuntos
Radioisótopos do Iodo , Sistema Fagocitário Mononuclear/fisiologia , Fagocitose , Salmonella typhimurium , Animais , Autorradiografia , Fígado/diagnóstico por imagem , Radiografia , Ratos , Ratos Endogâmicos , Baço/diagnóstico por imagemAssuntos
Líquido Amniótico/fisiologia , Complexo Antígeno-Anticorpo/metabolismo , Glomerulonefrite/metabolismo , Doenças do Complexo Imune/metabolismo , Glomérulos Renais/patologia , Animais , Feminino , Mesângio Glomerular/patologia , Glomerulonefrite/imunologia , Glomerulonefrite/patologia , Doenças do Complexo Imune/patologia , Camundongos , Camundongos Endogâmicos , Sistema Fagocitário Mononuclear/fisiologia , Gravidez , alfa-Fetoproteínas/fisiologiaRESUMO
The improvement of phagocytic activity of organs was studied in rats by means of 99mTc sulfur colloid uptake. This function was measured in the newborn rat, 1-, 2-, 3-, and 4-week-old baby rats and in adult rats. Organ distribution of the 99mTc-S-colloid showed marked phagocytic activity of the liver in all age groups including the newborn period. Phagocytosis in bone marrow was markedly increased and that in spleen markedly decreased at the time of birth. However, rapid improvement of phagocytic activity with age was prominent in the spleen.