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BACKGROUND: Human multi-tissue banks (HMTB) are important health institutions specialized in the capture, processing, and distribution of human tissues for transplants and research, aiming for safety and quality in the supply of their products, intended for reconstructive surgeries and injury repair, in addition to contributing to the advancement of research developed in regenerative medicine. This study aims to report and share the experience of implementing an HMTB, as well as creating an institution's own quality management system. METHODS: This is a descriptive study, an experience report type, which identifies historical aspects of an HMTB's actions in the 5 years of implementing and operating the service. RESULTS: Initially, a musculoskeletal tissue bank was established in collaboration with the Department of Orthopedics at the State University of Campinas, São Paulo, Brazil, in June 2018. In 2023, through a management model and associated technologies, the banks of human musculoskeletal and ocular tissues at the institution established the HC-UNICAMP Human Multi-tissue Bank. CONCLUSIONS: The implementation of the HMTB with modern and technological infrastructure, associated with the development and operation of the quality management system, allowed us to provide excellent organization of work processes, as well as obtain the necessary health license to begin activities. It is believed that this report can be an important source of information and recommendations applicable to the implementation of other human HMTBs.

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In this study, we sought to determine the prevalence of musculoskeletal injuries, perceived pain, and physical activity level among Brazilian practitioners of strength training (ST) and functional fitness (FF). Participants were 311 men and women who trained in 10 FF training centers and seven ST gyms. Each participant completed surveys of the prevalence of musculoskeletal injuries, their pain perception, and their physical activity level. A chi square test was used to analyze associations between groups and distributions of injuries. When any significant difference was observed, the difference score was analyzed through the adjusted residual values. Fisher's exact test was used to determined the associations between musculoskeletal injury prevalence and training modality (FF and ST) and between musculoskeletal injury prevalence and practice frequency (times/wk). To measure the magnitude of association between variables, the Phi coefficient was calculated for 2x2 associations and Cramer's V was used whenever the distributions were outside this standard. When the dependent variable presented a dichotomous characteristic, an Odds Ratio (OR) was calculated with a confidence interval of 95%. We found a higher musculoskeletal injury prevalence in the axial skeleton (n = 52; 83.88%) in FF practitioners and in the lower limbs of ST practitioners (n = 9; 52.96%). When the physical activity level cutoff point was set at 300 minutes per week, there was a significant relationship between physical activity and training modality (p = 0.005). There was also a significant association between pain perception and musculoskeletal injury (p < 0.001). Clinical follow-up was a protective factor to being injured (OR = 0.18; CI = 0.06-0.49), and, even after multivariate analysis this significant association was maintained (OR = 0.03; CI = 0.01 - 0.08). Thus, FF practitioners reported more musculoskeletal injuries than STs, and follow-up medical or physical therapy was a protective factor to these injuries. FF practitioners also had a higher level of weekly physical activity weekly than ST practitioners. Functional fitness practitioners may be at a higher risk of injuries than those who participate in traditional strength training.

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Abstract Skeletal muscle tissue has the largest mass in the human body, accounting for 45% of the total weight. Muscle injuries can be caused by bruising, stretching or laceration. The current classification divides these injuries into mild, moderate and severe. The signs and symptoms of grade I lesions are edema and discomfort; grade II, loss of function, gaps and possible ecchymosis; and grade III, complete rupture, severe pain and extensive hematoma. The diagnosis can be confirmed by ultrasound, which is dynamic and cheap, but examiner dependent; and magnetic resonance imaging (MRI), which provides better anatomical definition. The initial phase of the treatment consists in protection, rest, optimal use of the affected limb, and cryotherapy. Nonsteroidal anti-inflammatory drugs (NSAIDs), ultrasound therapy, strengthening and stretching after the initial phase and range of motion without pain are used in the clinical treatment. On the other hand, surgery has precise indications: hematoma drainage and muscle-tendon reinsertion and reinforcement.

Resumo O tecido muscular esquelético possui a maior massa do corpo humano, correspondendo a 45% do peso total. As lesões musculares podem ser causadas por contusões, estiramentos ou lacerações. A atual classificação separa as lesões entre leves, moderadas e graves. Os sinais e sintomas das lesões grau I são edema e desconforto; grau II, perda de função, gap e equimose eventual; grau III, rotura completa, dor intensa e hematoma extenso. O diagnóstico pode ser confirmado por ultrassom (dinâmico e barato, porém examinador-dependente); e ressonância magnética (RM) (maior definição anatômica). A fase inicial do tratamento se resume à proteção, ao repouso, ao uso otimizado do membro afetado e crioterapia. Anti-inflamatórios não hormonais (AINHs), ultrassom terapêutico, fortalecimento e alongamento após a fase inicial e amplitudes de movimento sem dor são utilizados no tratamento clínico. Já o cirúrgico possui indicações precisas: drenagem do hematoma, reinserção e reforço musculotendíneos.

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Despite the recent decrease in high tempo combat operations, threats to the medical readiness of Service Members remain a persistent issue. In fact, recent research efforts have demonstrated that musculoskeletal disease nonbattle injury represents perhaps the most immediate threat to the medical readiness of Service Members over the past several years. Innovations in a number of therapeutic options, particularly orthobiologics, have shown substantial promise in accelerating recovery and returning tactical athletes to full, unrestricted duties. Posttraumatic osteoarthritis remains a vexing topic but at the same time an intersectional opportunity for a multidisciplinary approach to better understand its pathogenesis, limit its prevalence, and mitigate the functional consequences of its sequalae. The expansion of a clinical infrastructure capable of the prospective collection of Service Members' functional outcomes across military treatment facilities promises to sharpen clinicians' understanding of both the impact of novel treatments for common injuries and the success of efforts to prevent recurrence (Military Orthopaedics Tracking Injury Network, Bethesda, MD). However, policy makers and stakeholders will increasingly find themselves in an environment of increasingly limited resources, which will necessitate creative strategies to maintain the lethality of a fit, fighting force.

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OBJECTIVE: Verify the incidence and rate of joint and muscle injuries with the practice of CrossFit® and the potential risk factors for injuries in men and women. METHODS: A total of 184 CrossFit® participants (97 men and 87 women). A questionnaire was conducted based on characterization, training routine, injuries, and additional information. The incidence refers to the number of new cases of injury in the sample during the CrossFit® training. The injury rate was calculated by estimating the number of weekly training hours and converted into the number of injuries per 1000 training hours. RESULTS: Among the participants, 38.6% reported some type of injury, being joint injuries (83.1%) and/or muscle injuries (45.1%). The injury rate was 3.4/1000 h, with men having a higher rate. The location of the joint injuries was shoulders, wrists, and elbows, in addition to the lumbar spine in women. As for muscle injuries, the deltoid, upper back muscles, and quadriceps femoris were the most reported in men and deltoid, trapezius, upper back muscles, and gastrocnemius in women. CONCLUSIONS: A better understanding of the anatomical distribution of the injuries may help coaches prescribe the most adequate workout that may reduce the likelihood of injuries occurring in CrossFit® training participants. It was demonstrated that gender, experience, weekly frequency, and previous injuries are important factors, and professionals must be concerned with the assessment of the participants and with an individualized progression in order to avoid risks.

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Purpose: To evaluate the impact of one-time high load exercise on skeletal muscle injury and analysis its mechanism in different genders. Methods: Twenty-four male and 24 female rats were divided randomly into four groups respectively: control, 0 h, 6 h, and 24 h after exercise. The activities of creatine kinase (CK), lactate dehydrogenase (LDH), and myohemoglobin (MYO) in serum, the expression level of oxidative stress markers, mitochondrial respiratory chain complex enzyme, and the apoptosis related protein in quadriceps were detected. Results: The results showed that the activities of CK, LDH and MYO in serum increased immediately after exercise and restored faster in female rats. More obvious structural disorder and apoptosis in male rats were showed. Malondialdehyde (MDA) and superoxide dismutase (SOD) were increased while catalase (CAT) and glutathione (GSH) were decreased in male rats. SOD, CAT and GSH were increased in female rats. Mitochondrial complex enzyme activity was decreased in males and increased in females. Conclusions: The skeletal muscle injury in both genders of rat could be induced by one-time high load exercise due to the mitochondrial respiratory enzyme dysfunction and oxidative stress, which was relatively mild and recovered quicker in female rats.

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OBJECTIVE: The aim of this systematic review was to investigate the effect of exercise-based programmes in the prevention of non-contact musculoskeletal injuries among football players in comparison to a control group. DESIGN: Systematic review and meta-analysis of randomised controlled trials. DATA SOURCES: MEDLINE, EMBASE, CENTRAL, CINAHL, PEDro and SPORTDiscus databases were searched from the earliest record to January 2021. ELIGIBILITY CRITERIA: Studies were eligible if they (1) included football players aged 13 years or older, (2) used exercise-based programmes as intervention, (3) presented the number of non-contact musculoskeletal injuries (ie, defined as any acute sudden onset musculoskeletal injury that occurred without physical contact) and exposure hours for each group, and (4) had a control group (eg, usual training, minimal intervention, education). All types of exercise-based prevention programmes were eligible for inclusion. Risk of bias for each included study and overall quality of evidence for the meta-analysis were assessed. RESULTS: Ten original randomised controlled trials with 13 355 football players and 1 062 711 hours of exposure were selected. Pooled injury risk ratio showed very low-quality evidence that exercise-based prevention programmes reduced the risk of non-contact musculoskeletal injuries by 23% (0.77 (95% CI 0.61 to 0.97)) compared with a control group. CONCLUSION: Exercise-based prevention programmes may reduce the risk of non-contact musculoskeletal injuries by 23% among football players. Future high-quality trials are still needed to clarify the role of exercise-based programmes in preventing non-contact musculoskeletal injuries among football players. PROSPERO REGISTRATION NUMBER: CRD42020173017.

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As injeções anestésicas perineurais são frequentemente utilizadas para auxiliarem na identificação dos locais potencialmente causadores de dor e consequentemente claudicação nos equinos. Nesse sentido, o conhecimento anatômico detalhado das estruturas neurais é importante para realização correta e eficiente dos bloqueios perineurais nos membros locomotores de equinos, não gerando dúvidas em suas interpretações. Para tanto, a ultrassonografia torna-se uma ferramenta importante no processo de aprendizagem, além de apontar a melhor forma de realizar o procedimento. Esta revisão tem por objetivo apresentar os fatores que interferem na realização das injeções perineurais e como a ultrassonografia pode ser útil no processo de aprendizagem pode auxiliar na execução destes procedimentos.(AU)

Perineural anesthetic injections are often used to assist in the identification of potentially painful sites and, consequently, lameness in horses. In this sense, detailed anatomical knowledge of neural structures is important for the correct and efficient performance of perineural blocks in the limbs of horses, generating no doubts in their interpretations. For this, ultrasonography becomes an important tool in the learning process, in addition to pointing out the best way to perform the procedure. This review aims to present the factors that interfere with the performance of perineural injections and how ultrasound can be useful in the learning process can assist in the execution of these procedures.(AU)

Las inyecciones de anestesia perineural a menudo se usan para ayudar en la identificación de sitios potencialmente dolorosos y, en consecuencia, la cojera en los caballos. En este sentido, el conocimiento anatómico detallado de las estructuras neurales es importante para el funcionamiento correcto y eficiente de los bloqueos perineurales en las extremidades locomotoras de los caballos, sin generar dudas en sus interpretaciones. Para esto, la ecografía se convierte en una herramienta importante en el procesode aprendizaje, además de señalar la mejor manera de realizar el procedimiento. Esta revisión tiene como objetivo presentar los factores que interfieren con el rendimiento de las inyecciones perineurales y cómo la ecografía puede ser útil en el proceso deaprendizaje puede ayudar en la ejecución de estos procedimientos.(AU)

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BACKGROUND: CrossFit® is usually composed of high intensity workout routines and is executed quickly, repetitively and with limited rest time. Previous studies have identified a high prevalence of injuries in CrossFit®. This study aimed to determine the prevalence of CrossFit-related musculoskeletal injuries and to identify potential associated factors. METHODS: A cross-sectional study was conducted with 413 CrossFitters. Participants completed a questionnaire containing personal data, training characteristics and injury history in the last 12 months. Data were analyzed by descriptive statistics and logistic regression models. RESULTS: The prevalence of CrossFit-related musculoskeletal injuries was 24.0%; and the injury rate was of 0.80 injuries per 1,000 hours of exposure. The regions of the body most affected were the lumbar spine (33.3%), shoulders (31.3%) and knees (14.1%). The majority of CrossFitters participated in competitions (74.6%), had more than 12 months of experience in CrossFit® (62.7%), and trained up to 90 minutes a day (82.3%) for more than 4 days a week (76.8%). The variables that showed a significant association with CrossFit®-related musculoskeletal injuries were weekly training frequency (OR=2.25; 95% CI: 1.13-4.48) and regular physiotherapeutic care (OR=1.85; 95% CI: 1.11-3.07). CONCLUSIONS: The prevalence of musculoskeletal injury was 24.0%, and the most affected regions of the body were the lumbar spine, shoulders and knees. Training more than four days a week and do not receive regular physiotherapeutic care were associated with CrossFit-related musculoskeletal injuries.

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OBJECTIVE: Taekwondo is a martial art that emphasizes blows using the feet and fists, and it is characterized by direct and continuous body contact, which subjects their practitioners to a higher number of injuries. This study aimed to determine the incidence of musculoskeletal injuries in Portuguese taekwondo athletes and analyze its associated factors. METHODS: The sample included 341 taekwondo athletes, aged between 4 and 62 years (18.77±12.77 years), 237 (69.5%) were male, and 104 (30.5%) female. A questionnaire was administered at a national level in taekwondo training and competitions via interview. RESULTS: One hundred and thirty-two (38.7%) taekwondo athletes reported having suffered an injury since they began their practice, totaling 294 injuries. Seventy-six (22.3%) athletes had an injury in the previous 12-months period, with a total of 112 injuries. There were 2.15 injuries per 1,000 hours of taekwondo training. The most common of all injuries was muscle injury (strain, contusion) (58.6%), in the foot and fingers (18.9%). The attack technique (28.8%) was the most prevalent injury mechanism. Adult athletes presented a higher risk of sustaining taekwondo-related injuries than adolescents (odds ratio = 3.91; 95%CI: 1.13-13.55; p=0.032), and athletes who trained more than 1 hour had a risk 4.20 times greater (95%CI: 1.44-12.29; p=0.009) than those who trained up to 1 hour per session. CONCLUSIONS: Injuries were frequent among Portuguese taekwondo athletes, with specific body areas affected, mainly caused by the attack technique. It is necessary to create injury prevention strategies, including specific training and the use of protective equipment.

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