RESUMO
INTRODUCTION: Several epidemiologic studies in humans have shown a relationship between pregestational obesity and congenital malformations in offsprings. However, there are no experimental evidence in animal models of obesity and pregnancy that reproduce the teratogenesis induced by this pathological condition. OBJECTIVE: To evaluate the effect of monosodium glutamate-induced obesity on embryonic development. METHODS: Female rats received subcutaneously (4 mg/g body weight) monosodium glutamate (MSG) solution or saline solution 0.9% (vehicle control) at days 2, 4, 6, 8, and 10 of life. At 90 days of age, all animals were mated, and on day 11 of pregnancy, the animals were killed. Biochemical variables (glucose, triglycerides, total cholesterol, and insulin) were determined in plasma of dams and embryo homogenates (DNA and protein content, advanced oxidation protein products). Embryos were evaluated for malformations, crown-rump length, and somite number. RESULTS: Obese rats presented higher triglyceride levels as compared to nonobese rats. Increased proportion of malformed embryos, decreased crown-rump length, somite number, DNA, and protein content were observed in offspring of obese rats. CONCLUSION: The model of obesity induced with MSG reproduces the maternal obesity-induced teratogenesis. The hypertriglyceridemia observed in MSG obese pregnant rats could be related to increased birth defect.
Assuntos
Doenças Fetais/metabolismo , Obesidade/embriologia , Obesidade/metabolismo , Complicações na Gravidez , Animais , Glicemia/metabolismo , Colesterol/sangue , Estatura Cabeça-Cóccix , Modelos Animais de Doenças , Feminino , Doenças Fetais/etiologia , Doenças Fetais/patologia , Insulina/sangue , Masculino , Obesidade/induzido quimicamente , Obesidade/complicações , Gravidez , Ratos , Ratos Wistar , Glutamato de Sódio/toxicidade , Somitos/patologia , Triglicerídeos/sangueRESUMO
The Mayer-Rokitansky-Kuster-Hauser is a rare congenital anomaly characterized by lack of vaginal and uterine development variable and normal ovaries. It results from agenesis or hypoplasia Müller duct system. Cervicovaginal agenesis as part of the complex syndrome, is even rarer. We report two cases: adolescent patient with primary amenorrhea, cervicovaginal agenesis and chronic pelvic pain, and a 28-year-old patient with primary amenorrhea, congenital absence of uterus and vagina.