RESUMO
AIM: To evaluate the mRNA expression levels of the cytokines interferon-γ, tumour necrosis factor-α, interleukin (IL)-1ß, IL-10, IL-6, VEGF, and AGT and the chemokine CCL2/MCP-1 in periapical interstitial fluid associated with root canal infections before and after the reduction of the bacterial load using a cleaning procedure. METHODOLOGY: The case group included 11 patients with chronic liver disease, and the control group included 11 healthy patients. Clinical samples were taken from teeth with pulp necrosis. After cleaning and drying the canal, three paper points were introduced into the root canal and passed through the root apex (2 mm) into the periapical tissues for 1 min. The samples were collected immediately after root canal cleaning and 7 days later to characterize those gene expression levels using real-time PCR. The data were subjected to the Shapiro-Wilk and the Wilcoxon tests. RESULTS: In the control group, significantly increased expression of the pro-inflammatory cytokines IFN-γ and TNF-α was observed in teeth with restrained bacterial loads (day 7) (P < 0.05). Similarly, increased TNF-α expression was found on day 7 in the liver group (P < 0.05). No differences were observed in the expression levels of the IL-1ß, IL-10 and, IL-6, MCP-1/CCL-2 and VEGF between the first collection (day 0) and second collection (day 7), over time in either group. CONCLUSION: Chronic liver disease patients exhibited sufficient immunologic ability showing relatively similar expression levels of cytokines, chemokines and angiogenic factors in periapical samples compared with the responses from no-chronic liver disease patients. The outcomes of this study suggest that liver impairment did not compromise the periapical immune response.
Assuntos
Quimiocinas/metabolismo , Citocinas/metabolismo , Hepatopatias/complicações , Hepatopatias/imunologia , Doenças Periapicais/imunologia , Tratamento do Canal Radicular , Dente/imunologia , Adulto , Idoso , Carga Bacteriana , Quimiocina CCL2/metabolismo , Cavidade Pulpar/microbiologia , Necrose da Polpa Dentária/imunologia , Feminino , Humanos , Interferon gama/metabolismo , Interleucina-10/metabolismo , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Masculino , Pessoa de Meia-Idade , Tecido Periapical/imunologia , Tecido Periapical/microbiologia , RNA Mensageiro/metabolismo , Ápice Dentário , Fator de Necrose Tumoral alfa/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismoRESUMO
This study investigated the presence of the Treponema species in longstanding endodontic retreatment-resistant lesions of teeth with apical periodontitis, the association of this species with clinical/radiographic features, and the association among the different target species. Microbial samples of apical lesions were collected from twenty-five adult patients referred to endodontic surgery after unsuccessful root canal retreatment. Nested-PCR and conventional PCR were used for Treponema detection. Twenty-three periradicular tissue samples showed detectable levels of bacterial DNA. Treponema species were detected in 28% (7/25) of the cases. The most frequently detected species were T. socranskii (6/25), followed by T. maltophilum (3/25), T. amylovorum (3/25), T. lecithinolyticum (3/25), T. denticola (3/25), T. pectinovorum (2/25) and T. medium (2/25). T. vicentii was not detected in any sample. Positive statistical association was found between T. socranskii and T. denticola, and between T. maltophilum and T. lecithinolyticum . No association was detected between the presence of any target microorganism and the clinical or radiographic features. Treponema spp. are present, in a low percentage, in longstanding apical lesions from teeth with endodontic retreatment failure.
Assuntos
Periodontite Periapical/microbiologia , Periodontite Periapical/terapia , Tecido Periapical/microbiologia , Treponema/isolamento & purificação , Infecções por Treponema/microbiologia , Adolescente , Adulto , Idoso , Distribuição de Qui-Quadrado , DNA Bacteriano/análise , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Periodontite Periapical/diagnóstico por imagem , Periodontite Periapical/patologia , Tecido Periapical/diagnóstico por imagem , Tecido Periapical/patologia , Reação em Cadeia da Polimerase , Radiografia , Retratamento , Tratamento do Canal Radicular , Falha de Tratamento , Infecções por Treponema/diagnóstico por imagem , Adulto JovemRESUMO
This study investigated the presence of the Treponema species in longstanding endodontic retreatment-resistant lesions of teeth with apical periodontitis, the association of this species with clinical/radiographic features, and the association among the different target species. Microbial samples of apical lesions were collected from twenty-five adult patients referred to endodontic surgery after unsuccessful root canal retreatment. Nested-PCR and conventional PCR were used for Treponema detection. Twenty-three periradicular tissue samples showed detectable levels of bacterial DNA. Treponema species were detected in 28% (7/25) of the cases. The most frequently detected species were T. socranskii (6/25), followed by T. maltophilum (3/25), T. amylovorum (3/25), T. lecithinolyticum(3/25), T. denticola (3/25), T. pectinovorum (2/25) and T. medium(2/25). T. vicentii was not detected in any sample. Positive statistical association was found between T. socranskiiand T. denticola,and between T. maltophilumand T. lecithinolyticum. No association was detected between the presence of any target microorganism and the clinical or radiographic features. Treponemaspp.are present, in a low percentage, in longstanding apical lesions from teeth with endodontic retreatment failure.
Assuntos
Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Periodontite Periapical/microbiologia , Periodontite Periapical/terapia , Tecido Periapical/microbiologia , Treponema/isolamento & purificação , Infecções por Treponema/microbiologia , Distribuição de Qui-Quadrado , DNA Bacteriano/análise , Reação em Cadeia da Polimerase , Periodontite Periapical/patologia , Periodontite Periapical , Tecido Periapical/patologia , Tecido Periapical , Retratamento , Tratamento do Canal Radicular , Falha de Tratamento , Infecções por TreponemaRESUMO
Anachoresis is the phenomenon through which blood-borne bacteria, dyes, pigments and other materials are attracted and fixed to circumscribed areas of inflammation. This study evaluated the occurrence of anachoresis in the periapical region of dogs submitted to root canal fillings. One hundred and four roots from four dogs were endodontically treated and root canals were filled with zinc-oxide-eugenol cement. Fifty percent were filled up to the dentinocemental junction and the others were overfilled. At 120 days after root canal treatment, experimental bacteremia was induced by intravenous inoculation of 105 CFU Streptococcus pyogenes. The dogs were sacrificed 48 hours and 30 days after the bacteremia. Culture and DNA amplification by PCR revealed the presence of the inoculated bacteria just in periapical tissues of dogs sacrificed 48 hours after bacteremia and not in animals sacrificed after 30 days. AP-PCR fingerprints of recovered colonies of S. pyogenes and the presence of genetic markers of resistance to antimicrobials were similar to the inoculated strain. Endodontically treated periapices seemed to be prone to the occurrence of anachoresis and there was no relationship between the phenomenon and the level of root canal filling.
Anacoresis es el fenómeno por el cual las bacterias transmitidas por la sangre, colorantes, pigmentos y otros materiales se atraen y se fija a zonas circunscritas de la inflamación. Este estudio evaluó la incidencia de anacoresis en la región periapical de los perros presentados a raíz de los rellenos del canal. Un total de ciento cuatro raíces de cuatro perros fueron tratados con endodoncia y tratamientos de conducto se rellena con cemento de óxido de zinc-eugenol. El cincuenta por ciento estaban llenos hasta el cruce dentinocemental y los otros se llene en exceso. A los 120 días después del tratamiento de conducto radicular, bacteriemia experimental fue inducida por la inoculación intravenosa de 105 UFC por Streptococcus pyogenes. Los perros fueron sacrificados 48 horas y 30 días después de la bacteriemia. La cultura y la amplificación del ADN por PCR reveló la presencia de las bacterias inoculadas sólo en los tejidos periapicales de los perros sacrificados 48 horas después de la bacteriemia y no en los animales sacrificados después de 30 días. AP-PCR huellas dactilares de las colonias recuperadas de S. pyogenes y la presencia de marcadores genéticos de resistencia a los antimicrobianos fueron similares a la cepa inoculada. Periápices endodonciados parecía ser propensos a la ocurrencia de anacoresis y no había ninguna relación entre el fenómeno y el nivel de llenado del conducto radicular.
Assuntos
Cães , Bacteriemia/microbiologia , Obturação do Canal Radicular , Streptococcus pyogenes/isolamento & purificação , Tecido Periapical/microbiologia , DNA Bacteriano/genética , Cavidade Pulpar/microbiologia , Reação em Cadeia da Polimerase , Streptococcus pyogenes/genéticaRESUMO
The objective of this study was to determine the ecological relationships between bacterial species that colonize infected root canals. Root canal bacteria recovered from one patient with pulp canal necrosis were evaluated in vitro for synergistic and antagonistic activities determined by mono and co-culture growth kinetics and the production of bacteriocin-like substances using the double layer diffusion method. Peptostreptococcus prevotii triggered a significant increase of Fusobacterium nucleatum growth, while the former bacteria did not affect the growth of P. prevotii. The bacterial species did not produce antagonism activity against itself or against any of the other two species. Despite many studies have demonstrated the capability of root canal microorganisms to produce antagonistic substances, these in vitro experimental tests show the synergistic effect of P. prevotii on the growth of F. nucleatum.
Assuntos
Humanos , Cavidade Pulpar/crescimento & desenvolvimento , Cavidade Pulpar/microbiologia , Endodontia , Infecções por Fusobacterium , Fusobacterium nucleatum/crescimento & desenvolvimento , Infecções por Bactérias Gram-Positivas , Técnicas In Vitro , Peptostreptococcus/crescimento & desenvolvimento , Tecido Periapical/crescimento & desenvolvimento , Tecido Periapical/microbiologia , Sinergismo Farmacológico , Métodos , Microbiologia , MétodosRESUMO
The aim of this study was the radiographic evaluation of the apical and periapical region of dog teeth submitted to intracanal bacterial endotoxin (lipopolysaccharide, LPS), associated or not with calcium hydroxide. After removal of the pulp, 60 premolars were divided into four groups and were filled with bacterial endotoxin (group 1), bacterial endotoxin plus calcium hydroxide (group 2), saline solution (group 3), or periapical lesions were induced with no treatment (group 4), for a period of 30 days. Similar periapical lesions were observed in groups 1 and 4. The lamina dura was intact in groups 2 and 3. Bacterial endotoxin (LPS) caused radiographically visible periapical lesions, but when associated with calcium hydroxide, this endotoxin was detoxified.
Assuntos
Hidróxido de Cálcio/uso terapêutico , Endotoxinas/efeitos adversos , Escherichia coli , Lipopolissacarídeos/efeitos adversos , Tecido Periapical/diagnóstico por imagem , Materiais Restauradores do Canal Radicular/uso terapêutico , Ápice Dentário/diagnóstico por imagem , Animais , Membrana Basal/diagnóstico por imagem , Reabsorção Óssea/diagnóstico por imagem , Reabsorção Óssea/microbiologia , Cães , Endotoxinas/antagonistas & inibidores , Lipopolissacarídeos/antagonistas & inibidores , Doenças Periapicais/diagnóstico por imagem , Doenças Periapicais/microbiologia , Tecido Periapical/microbiologia , Ligamento Periodontal/diagnóstico por imagem , Radiografia , Reabsorção da Raiz/diagnóstico por imagem , Reabsorção da Raiz/microbiologia , Estatísticas não Paramétricas , Ápice Dentário/microbiologiaRESUMO
As bactérias constituem fatores primordiais da contaminaçäo da polpa dentária de dentes decíduos resultando em implicaçöes clínicas e terapêuticas. Com a finalidade de analisar a presença de bactérias planctônicas, colônias e biofilmes microbianos nas estruturas de dentes decíduos portadores de pulpite e necrose pulpar, utilizaram-se 32 dentes decíduos com cárie profunda. Dezesseis dentes foram seccionados no sentido longitudinal e o restante no sentido transversal. Os espécimes foram corados pela hematoxilina-eosina de Harris e Brown e Brenn para análise pela microscopia óptica. A partir da metodologia empregada pôde-se verificar que: 1) Em dentes decíduos com pulpite decorrente de cárie dentária, as bactérias podem ser encontradas nos túbulos dentinários e especialmente no interior do tecido pulpar na área inflamada. Colônias bacterianas e biofilmes microbianos säo visualisados na superfície cariosa e nos focos de liquefaçäo dentinária; 2) As bactérias plactônicas, colônias bacterianas e biofilmes microbianos estäo abundantemente presentes nas câmaras pulpares e canais radiculares de dentes decíduos com necrose pulpar, localizando-se nas superfícies do teto, do soalho da câmara pulpar, nas paredes laterais do canal radicular e, por extensäo, nas paredes externas apicais...
Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Bactérias Anaeróbias/isolamento & purificação , Cavidade Pulpar/microbiologia , Bactérias Anaeróbias/classificação , Dente Decíduo/patologia , Necrose da Polpa Dentária/patologia , Patologia Bucal , Tecido Periapical/microbiologia , Pulpite/patologiaRESUMO
Lesöes periapicais crônicas foram avaliadas pela técnica de imunofluorescência indireta com o propósito de estimar a presença de anticorpos IgG específicos para o Bacteroides gingivalise Bacteroides intermedius. Das 15 amostras examinadas, 7 (46,6 por cento) apresentaram reatividade anticórpica
Assuntos
Humanos , Masculino , Feminino , Anticorpos , Bacteroides , Imunofluorescência , Tecido Periapical/lesões , Tecido Periapical/microbiologia , Porphyromonas gingivalis , Prevotella intermediaRESUMO
Estudia el papel de los fenómenos en el establecimiento y mantenimiento o reparación de las patologías periapicales basandose en la revisión bibliográfica existente, se evalúa lo ya reportado y se aportan conclusiones sobre aspectos nuevos de esa información