RESUMO
The objective of the present study was investigate the prevalence of dermatophytes in dogs, cats and environment floor through molecular epidemiology tools to identify the genetic profile of these infectious agents. This was an observational study with cross-sectional surveys design. Sample were collected from the hair and skin of 52 dogs and cats with the clinical suspicion of dermatophytosis, over a period of one year in Maringá, in the state of Paraná, Brazil. Household samples (carpets and floor), were collected from animals that were positive for dermatophytosis by morphological colonies characteristics, and samples of dogs or cats living in the same household as with the positive animals were also collected. After mycological confirmation, molecular typing was performed by random amplified polymorphic DNA (RAPD). Microsporum canis was the unic dermatophyto isolated whose prevalence was 26.9% (14/52) in animals with the clinical suspicion of dermatophytosis and four other animals that lived with positive animals. As some animals had more than one lesion site, there were 22 total positive cultures from samples from animals and another ten from abiotic sources. The majority of the animals that provided positive cultures for M. canis were aged up to five months (77.8%) and were female (66.7%). Molecular typing using the P1 primer revealed genetically distinct profiles in the symptomatic, asymptomatic and environmental animal samples, or the same animal, furthermore, showed that M. canis could have microevolution.
Assuntos
Doenças do Gato/microbiologia , Doenças do Cão/microbiologia , Microsporum/isolamento & purificação , Tinha/veterinária , Animais , Brasil/epidemiologia , Doenças do Gato/epidemiologia , Gatos , Doenças do Cão/epidemiologia , Cães , Feminino , Masculino , Tinha/parasitologiaRESUMO
Los dermatofitos son hongos filamentosos queratinofílicos, que pueden actuar como agentes zoonóticos; no obstante, aún se debe determinar el papel que cumplen los animales salvajes en la transmisión de las dermatofitosis. El objetivo de este trabajo fue estudiar la presencia de dermatofitos en los pelos de animales de la fauna brasileña. Fueron analizados treinta y dos mamíferos salvajes sanos de varios taxones - de los cuales 17 se hallaban en cautiverio y 15 en vida libre. Las muestras se obtuvieron por fricción con trozos de alfombras estériles que, una vez pasados por el pelo de los animales, fueron sembrados en agar Mycobiotic e incubados a 25 "C. Las colonias se identificaron de acuerdo a sus características macro y microscópicas. Se aislaron dermatofitos del 9,5% de los animales estudiados: Microsporum gypseum de un aguará guazú (Chrysocyon brachyurus), Microsporum cookie de un coatí (Nasua nasua) y Trichophyton ajelloi de un perro del monte (Speothos venaticus). En relación a la salud pública, estos animales representan uma posible fuente de infección para el hombre y otros animales.
Dermatophytes are keratinophilic fungi that can cause zoonosis. However, the role wild animals play in the transmission of these infections is yet to be determined. The aim of this study was to determine the presence of dermatophytes on the haircoat of Brazilian wild mammals. Thirty-two healthy wild mammals from several taxa were studied: 17 were captive and 15 were free-living individuals. Samples were obtained by rubbing the haircoat with sterile carpets. Samples were cultured on Mycobiotic agar, and the plates were incubated at 25 "C. Identification of the isolates was carried out on the basis of macro-and micromorphology. Dermatophytes were isolated from 9.5% of the animals: Microsporum gypseum from one maned wolf (Chrysocyon brachyurus), Microsporum cookie from one coati (Nasua nasua), and Trichophyton ajelloi from one bush dog (Speothos venaticus). These animals represent therefore sources of infection for both humans and other animals and are important for public health policies.
Dermatófitos são fungos filamentosos queratinofílicos e agentes de zoonoses; entretanto, o papel que os animais selvagens representam na transmissão das dermatofitoses ainda precisa ser determinado. O objetivo deste trabalho foi pesquisar dermatófitos no pelame de animais da fauna brasileira. Trinta e dois mamíferos selvagens sadios de vários táxons foram estudados, 17 de cativeiro e 15 de vida livre. As amostras foram obtidas por fricção de carpetes estéreis no pelame dos animais. As amostras foram semeadas em ágar Mycobiotic e incubadas a 25 "c, identificando-se as colônias por suas características macro e microscópicas. Isolaram-se dermatófitos de 9,5% dos animais pesquisados: Microsporum gypseum de um lobo-guará (Chrysocyon brachyurus), Microsporum cookie de um quati (Nasua nasua) e Trichophyton ajelloi de um cachorro-do-mato-vinagre (Speothos venaticus). Esses animais representam fontes de infecção para homens e outros animais, sendo importantes para a saúde pública.
Assuntos
Animais , Arthrodermataceae/patogenicidade , Cabelo/parasitologia , Controle de Infecções , Mamíferos/parasitologia , Microsporum , Tinha/parasitologia , Tinha/prevenção & controle , Tinha/veterinária , TrichophytonRESUMO
Relato de caso de tinea corporis por Microsporum gypseum em paciente com Aids de 36 anos. Segundo a literatura, este dermatófito näo é um agente comum neste grupo de pacientes. As lesöes eram psoriasiformes, generalizadas e näo responderam ao tratamento com cetoconazol e itraconazol
Assuntos
Humanos , Adulto , Dermatomicoses/parasitologia , Microsporum/isolamento & purificação , Síndrome da Imunodeficiência Adquirida/complicações , Tinha/parasitologia , Psoríase/etiologiaRESUMO
En una área geografica que incluye la ciudad de Santiago y Valparaíso, analizamos 31 cepas de Microsporum canis aisladas desde niños prepuberes con dermatofitosis (24 desde cuero cabelludo y 7 de piel), con la finalidad de analizar su variación fenética en 5 medios de cultivo sólidos (Sabouraud, Lactritmel, Papa dextrosa, Czapek y Nitrito sucrosa agar). Se seleccionaron 19 características macro y microscópicas, 9 de ellas consideradas de valor taxonómico primario, las que se estudiaron en todos los medios a los 14 días de incubación a 27ºC, a partir de subcultivos provenientes de su primer aislamiento clínico. Los datos obtenidos de los 3 primeros medios de cultivos básicos, se analizaron estadísticamente. Solo se observaron coincidencias en estos tres medios, al considerar cada parámetro por separado, principalmente: presencia de microconidios (8/31), forma del macroconidio (10/31) y color del reverso (23/31). El medio que más discrimina es el Sabouraud, al producir las mayores diferencias entre cepas; en segundo lugar Papa dextrosa y, finalmente Lactritmel, que por su menor discriminación permite obtener más agrupaciones. En agar nitrito de sodio, se apareció la conidiogénesis más estable. Se determinó la presencia de 2 cepas de Microsporum canis sub. sp. pulverulentum, situación no registrada anteriormente en nuestro medio ambiente
Assuntos
Humanos , Pré-Escolar , Criança , Dermatomicoses/epidemiologia , Microsporum/isolamento & purificação , Meios de Cultura/normas , Tinha/diagnóstico , Tinha/epidemiologia , Tinha/parasitologiaRESUMO
From 1979 to 1988, 3890 positive samples for dermatophytes obtained at the Dermatology Service of Hospital San Juan de Dios (Western Santiago area) were analyzed for type of fungus involved and for relative yield of direct examination compared to culture. Direct examination was performed on samples treated with 20% KOH and stained with rhodamine. Cultures were obtained on Agar Sabouraud medium with addition of gentamycin and chloramphenicol. Yield of direct examination was 97.2% compared to 85% for culture. According to anatomic location, M canis was isolated from nearly all cases of T capitis. In cases of T pedis, nail mycosis, crural and palmar infections the most frequent species were T rubrum and T mentagrophytes M canis and T rubrum were responsible for infections of the body.