RESUMO
The effects of emerging contaminants on environmental health are of high concern, especially those potentially induced by mixtures. We assessed single and composite mixtures of triclosan (T), 17ß-estradiol (E2), sulfamethoxazole (SMX), and nicotine (N) at various concentrations, on neonates of Daphnia magna. When used in single exposure, T and N induced high toxicity (100% immobility, each one), compared to SMX and E2 (2.5% and 10% immobility, respectively). When T, E2, SMX and N were in mixture, T had the highest contribution to the overall toxicity in mixture exposures. The N toxicity lowered when in a fourfold exposure (85% immobility in fourfold exposure). Due to the high toxicity of T and N, both alone and in the mixtures, our results can serve as a warning about the use of these substances and their release in the aquatic ecosystem.
Assuntos
Triclosan , Poluentes Químicos da Água , Animais , Daphnia , Ecossistema , Poluentes Químicos da Água/análise , Triclosan/toxicidade , SulfametoxazolRESUMO
Triclosan (TCS) is an antibacterial compound used mainly in personal care products. Its widespread use for decades has made it one of the most widely detected compounds in environmental matrices and in biological fluids. Although it has been shown to be an endocrine disruptor in rats and aquatic species, its safe use by humans is unclear. The aim of the present study was to evaluate the effects of exposure to TCS in female rats. To this end, 14 rats were divided into two groups and fed daily as follows: the control group with sesame oil and the TCS group at a dose of 50 mg/kg/day for 28 days. Any signs of toxicity in the rats were observed daily, and the weight and phase of the estrous cycle were recorded. At the end, the rats were decapitated, the serum and ovaries were collected. The levels of testosterone and progesterone in serum were determined by immunoassay and mass spectrometry. Estradiol (in serum) and kisspeptin-10 (in serum and ovary) were measured only by immunoassays. Trace elements were determined by inductively coupled plasma-mass spectrometry (ICP-MS). The weight gain study of the rats showed a significant decrease by exposure to TCS, while the estrous cycle was not significantly affected compared to the control. The optimized methods based on mass spectrometry showed a significant decrease in the levels of progesterone and testosterone due to exposure to TCS. In addition, elements determined by ICP-MS in rat serum showed significant changes in calcium, lithium and aluminum due to TCS treatment. Finally, the kisspeptin-10 levels did not show a negative effect due to the treatment by TCS. The results suggest that medium-term exposure to TCS did not significantly alter estrous cyclicity but caused alterations in growth, sex hormone levels and some elements in the rat serum.
Assuntos
Disruptores Endócrinos , Oligoelementos , Triclosan , Alumínio , Animais , Antibacterianos , Cálcio , Disruptores Endócrinos/toxicidade , Estradiol , Feminino , Hormônios Esteroides Gonadais , Humanos , Lítio , Progesterona , Ratos , Óleo de Gergelim , Testosterona , Triclosan/toxicidadeRESUMO
Triclosan (5-chloro-2'-[2,4-dichlorophenoxi]-phenol) is a polychlorinated biphenolic antimicrobial, utilized as antiseptic and preservative in hygiene products and medical equipment. Triclosan causes mitochondrial dysfunction (uncoupling, inhibition of electron flow), as demonstrated in isolated rat liver mitochondria. These actions in the mitochondria could compromise energy-dependent metabolic fluxes in the liver. For this reason, the present work aimed at investigating how these effects on isolated mitochondria translate to the whole and intact hepatocyte. For accomplishing this, the isolated perfused rat liver was utilized, a system that preserves both microcirculation and the cell-to-cell interactions. In addition, the single-pass triclosan hepatic transformation was also evaluated by HPLC as well as the direct action of triclosan on gluconeogenic enzymes. The results revealed that triclosan decreased anabolic processes (e.g., gluconeogenesis) and increased catabolic processes (e.g., glycolysis, ammonia output) in the liver, generally with a complex pattern of concentration dependences. Unlike the effects on isolated mitochondria, which occur in the micromolar range, the effects on intact liver required the 10-5 to 10-4 M range. The most probable cause for this behavior is the very high single-pass transformation of triclosan, which was superior to 95% at the portal concentration of 100 µM. The concentration gradient along the sinusoidal bed is, thus, very pronounced and the response of the liver reflects mainly that of the periportal cells. The high rates of hepatic biotransformation may be a probable explanation for the low acute toxicity of triclosan upon oral ingestion.
Assuntos
Triclosan , Animais , Metabolismo Energético , Gluconeogênese , Fígado , Mitocôndrias Hepáticas , Ratos , Triclosan/toxicidadeRESUMO
Triclosan (TCS) is a synthetic broad-spectrum antimicrobial agent commonly used world-wide in a range of personal care and sanitizing products detected frequently in aquatic ecosystems. The aim of this study was to examine biochemical markers responses triggered by TCS in Danio rerio and in a native South American fish species (Corydoras paleatus). Further, an integrated approach comparing both test fish species was undertaken. These fish organisms were exposed to 100 or 189 µg TCS/L for 48 h. The activities of catalase (CAT), glutathione-s-transferase (GST), superoxide dismutase (SOD), and lipid peroxidation levels (LPO) and total antioxidant capacity against peroxyl radicals (ACAP) were determined in liver, gills, and brain. Acetylcholinesterase activity (AChE) was measured in the brain. Multivariate analysis showed that the most sensitive hepatic parameters were activities of GST and SOD for C. paleatus while LPO levels were for D. rerio. In gills the same parameters were responsive for C. paleatus but CAT in D. rerio. ACAP and GST activity were responsive parameters in brain of both species. Integrated biomarker responses (IBR) index demonstrated similar trends in both species suggesting this parameter might serve as a useful tool for quantification of integrated responses induced by TCS.
Assuntos
Anti-Infecciosos Locais/toxicidade , Biomarcadores , Estresse Oxidativo/efeitos dos fármacos , Triclosan/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/enzimologia , Peixes-Gato , Brânquias/efeitos dos fármacos , Brânquias/enzimologia , Fígado/efeitos dos fármacos , Fígado/enzimologia , Peixe-ZebraRESUMO
Triclosan (TCS) is an antimicrobial and antimycotic agent widely used in personal care products. In aquatic environments, both TCS and its biomethylated more persistent form, methyl-triclosan (MeTCS), are usually detected in wastewater effluents and rivers, where are commonly adsorbed to suspended solids and sediments. The aim of this study was to evaluate biochemical and physiological effects in Danio rerio after a short term (2 days) and prolonged (21 days) exposures to sediment spiked with TCS acting as the source of the pollutant in the assay. The activities of catalase (CAT), glutathione-s transferase (GST) and superoxide dismutase (SOD), lipid peroxidation levels (LPO), total capacity against peroxyl radicals (ACAP), and acetylcholinesterase enzymatic activity (AChE) were measured in liver, gills, and brain. Most of TCS on the spiked sediment was biotransformed to MeTCS and promoted different adverse effects on D. rerio. Gills were the most sensitive organ after 2 day-exposure, showing lipid damage and increased SOD activity. After 21 days of exposure, liver was the most sensitive organ, showing lower ACAP, increased LPO levels, and SOD and CAT activities. This is the first study reporting the effects on biochemical markers in D. rerio from a MeTCS sink resulting from sediment spiked with TCS.
Assuntos
Triclosan , Poluentes Químicos da Água , Acetilcolinesterase , Animais , Catalase/metabolismo , Estresse Oxidativo , Superóxido Dismutase/metabolismo , Triclosan/toxicidade , Poluentes Químicos da Água/toxicidade , Peixe-Zebra/metabolismoRESUMO
Triclosan is an antimicrobial agent widely used in personal care products and an emerging contaminant with potential to have harmful effects to edaphic organisms. This study aimed to evaluate the impacts of exposure to triclosan on the microbiota, plants, and edaphic animals using isolated bioassays and a microcosm scale representation (multispecies system). Among the isolated bioassays, the phytotoxicity test with Lactuca sativa, avoidance test with Eisenia andrei, and acute toxicity with E. andrei and Armadillidium vulgare were used. The multispecies system used seeds of L. sativa and Sinapis alba, together with earthworms and terrestrial isopods. This system also evaluated microbial activity through alkaline phosphatase and the metabolic profile using Ecoplate™, BIOLOG microplates. Exposure to triclosan impacted seedling growth in the isolated bioassay and germination and root growth in the microcosm scale assay; it also caused mortality in terrestrial isopods, earthworm avoidance and alteration of alkaline phosphatase, and the consumption profile of carbohydrates and carboxylic acids in the microbiota. The ecotoxicological effects evaluated in the multispecies system were perceived even in low concentrations of triclosan, indicating that the interaction of this xenobiotic with the environment and organisms in a more realistic scenario can compromise ecosystem services.
Assuntos
Oligoquetos , Poluentes do Solo , Triclosan , Animais , Ecossistema , Solo , Poluentes do Solo/análise , Poluentes do Solo/toxicidade , Triclosan/toxicidadeRESUMO
The present study aimed to evaluate the effects of exposure for four months, with ibuprofen and triclosan at 25 and 50 µg/L in Striped catfish Pseudoplatystoma magdaleniatum, evaluated between sexes and exposure times. Biochemical biomarkers such as lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyltransferase, acetylcholinesterase, creatine kinase, lipid peroxidation, albumin, globulins, creatinine, and urea were evaluated. The results of this study suggest that both ibuprofen and triclosan at concentrations of 25 and 50 µg/L can cause alterations to P. magdaleniatum, interfering with the activity of certain enzymes associated with energy production, immune response, architecture, and cellular physiology. Also, we determined the current state of contamination in fish, the concentration of ibuprofen and triclosan in P. magdaleniatum muscle samples from the different places markets located on the banks of the main rivers of Colombia was quantified by UHPLC-QqQ-MS/MS, in three climatic periods; finding triclosan levels in the dry season in some of the sampling points compatible with enzyme-level alterations in this species.
Assuntos
Peixes-Gato , Triclosan , Animais , Colômbia , Ibuprofeno , Espectrometria de Massas em Tandem , Triclosan/toxicidadeRESUMO
Urban waste is a complex mixture of different substances, including microplastics and pharmaceuticals and personal care products. Microplastics have a high affinity for hydrophobic substances. One of these substances is triclosan, a bactericide used in a variety of hygiene products. Therefore, microplastics (MPs) may serve as a vector between triclosan and aquatic organisms. The current study sought to evaluate the effects of the interaction between microplastics and triclosan based on a mechanistic approach in which the oyster Crassostrea brasiliana was used as a model. The organisms were exposed to three conditions: the control, microplastic (MP), and microplastic contaminated with triclosan (MPT). The organisms were exposed for 3 or 7 days. After the exposure time, hemolymph was sampled for performing the neutral red retention time assay and, subsequently, the gills, digestive glands, and adductor muscles were dissected for measuring biomarkers responses (EROD, DBF, GST, GPx, GSH, lipid peroxidation, DNA strand breaks, and AChE). Our results demonstrate combined effects of MPs associated with triclosan on oyster physiology and biochemistry, as well as on lysosomal membrane stability. These results contribute to understanding the effects of contaminants of emerging concern and microplastics on aquatic organisms.
Assuntos
Crassostrea/efeitos dos fármacos , Biomarcadores Ambientais/efeitos dos fármacos , Microplásticos/toxicidade , Triclosan/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Brasil , Crassostrea/genética , Crassostrea/metabolismo , Dano ao DNA , Brânquias/efeitos dos fármacos , Brânquias/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Microplásticos/metabolismo , Modelos Teóricos , Triclosan/metabolismo , Poluentes Químicos da Água/metabolismoRESUMO
BACKGROUND: Triclosan (TCS) is an antimicrobial agent widely used in health care and consumer products. This compound is present in sludge of wastewater treatment plants (WWTPs), and because of its bactericidal characteristics, it can inhibit the methanogenic activity in anaerobic digestion (AD) technology. The aim of this study was to evaluate the toxic effects of TCS on the methanogenic activity. RESULTS: Batch anaerobic reactors were used with TCS concentrations of 7.8, 15.7, 23.5, and 31.4 mg/L. These assays consisted in three successive feedings (I, II, and III), wherein the sludge was exposed to each TCS concentration and volatile fatty acid (VFA) substrate. For evaluation of the residual sludge activity during feeding III, only VFA was used. The results showed that the increase in TCS concentrations correlated with the reduction in methane (CH4) production. In this case, the minimum values were achieved for TCS concentration of 31.4 mg/L with CH4 levels between 101.9 and 245.3 during feedings I, II, and III. Regarding the effect of TCS on VFA consumption, an inhibitory effect was detected for TCS concentrations of 23.5 and 31.4 mg/L, with concentrations of acetic, butyric, and propionic acids at the end of the assay (37 d) between 153.6 and 206.8, 62.5 and 60.1, and 93.4 and 110 mg/L, respectively. Regarding the removal of TCS during AD, these values were above 47%. Conclusion: TCS is an inhibitor of methanogenic activity with a decrease between 63 and 70% during the different feedings. The CH4 production was not recovered during feeding III, with inhibition percentages of 2172%.
Assuntos
Triclosan/toxicidade , Digestão Anaeróbia , Metano/metabolismo , Anti-Infecciosos/toxicidade , Esgotos , Estações de Tratamento de Águas Residuárias , Cromatografia Líquida de Alta Pressão , Ácidos Graxos Voláteis , AnaerobioseRESUMO
Triclosan (TCS) is a phenolic compound with antimicrobial action widely used in cosmetics and other personal care products and other industry segments. Its widespread use over the decades has made TCS one of the most commonly detected compounds in wastewater and effluent worldwide already being found in human urine, plasma and milk. In this study, the (anti)estrogenicity of TCS was evaluated in the uterotrophic assay in 18-day old female Wistar rats. In a second protocol, female rats were evaluated for the reproductive effects of TCS in a two-generation reproduction toxicity study. Female rats were daily treated by gavage with TCS at the doses of 0.8, 2.4 and 8.0 mg/kg/day or corn oil (control group) over 10 weeks (F0) and over 14 weeks (F1) prior to mating and then throughout mating, gestation and lactation until weaning of F1 and F2 generation respectively. TCS had no effect on the uterus weight in the uterotrophic assay. In the two-generation study, the TCS exposure compromised female sexual behavior, decreased maternal food consumption and increased pup grooming on TCS 2.4 group. The TCS chronic exposure also decreased the perimetrium thickness of F0 females from TCS 8.0 group and growing follicle number of TCS 2.4 females from F1 generation. Despite the some specific changes detected in the two-generation study, no impairment was observed in the uterotrophic assay and other important reproductive endpoints. In a weight of evidence evaluation, the results suggest that exposure to TCS at low doses did not act as an endocrine disruptor in the female rat reproductive system.