RESUMO
UNLABELLED: These ovarian neoplasm derive from the ovarian stromal component constituting around the 5 to 12% of all ovarian tumors. OBJECTIVE: To examine the histopathological and ultrastructural morphologic characteristic of the neoplastic cells and the patognomonic element of these tumors: Call Exner's Bodies MATERIALS AND METHODS: The materials corresponded to 2 women of 52 and 55 years. The syntomatology was abdominal tumor that went in increase. The materials were fractioned for the histopathological conventional study and for ultrastructural analysis. For this last one, they were fixed in Karnovsky, refix in osmio and included in Araldita. RESULTS: By means of the different observations it was determined in both cases the nuclear atipia, indentations nuclei and prominent nucleoli. In one of the cases the presence of Bodies of Call-Exner was detected, and ultrastructurally was compound by whirled fibrils and amorphous material, with dense structures electron to its around. It was interest the infiltrated of plasmatic cells around the tumors cells. These neoplasms are of interest due to their impredictible behavior and to the hormonal production that can originate alterations in other organs of the genital apparatus.
Assuntos
Tumor de Células da Granulosa/patologia , Tumor de Células da Granulosa/ultraestrutura , Neoplasias Ovarianas/patologia , Neoplasias Ovarianas/ultraestrutura , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
Estas neoplasias ováricas derivan del componente estromal ovárico, constituyendo alrededor del 5 al 12 por ciento del total de los tumores ováricos. Objetivo: Examinar a nivel histopatológico y ultraestructural las características morfológicas de las células neoplásicas y del elemento patognomónico de estos tumores: los Cuerpos de Call Exner. Material y Métodos: Los materiales correspondieron a 2 mujeres de 52 y 55 años- La sintomatología era tumoración abdominal la cual iba en aumento. Los materiales fueron fraccionados para el estudio histopatológico convencional y para el análisis electromicroscópico. Para este último, fueron fijados en Fijador Karnovsky , refijados en osmio e incluídos en Araldita. Resultados: por medio de las diferentes observaciones se determinó en ambos casos la atipia nuclear, núcleos indentados y nucleólos prominentes.. En uno de los casos se detectó la presencia de Cuerpos de Call-Exner, que ultraestructuralmente estaban compuestos por fibrillas arremolinadas y material amorfo, con estructuras electrón densas a su alrededor. Fue llamativo el infiltrado de células plasmáticas alrededor de las células tumorales . Estas neoplasias son de interés debido a su conducta impredecible y a la producción hormonal que puede originar alteraciones en otros sistemas del aparato genital. (Au)
Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Tumor de Células da Granulosa/ultraestrutura , Tumor de Células da Granulosa/patologia , Neoplasias Ovarianas/ultraestrutura , Neoplasias Ovarianas/patologiaRESUMO
Los tumores derivados del estrome gonadal diferenciado corresponden aproximadamente al 5 por ciento de las neoplasias ováricas y dentro de éstos, los tumores de las células de la granulosa (TCG) son los más frecuentes. Existen dos variedades de TCG, siendo la variedad juvenil una entidad histopatológicamente diferente y la menos común. El propósito de éste trabajo es mostrar nuestra experiencia en tres casos de tumores de las células de la granulosa juvenil (TCGJ), tanto en los aspectos clínicos, terapéuticos, como asimismo en los hallazgos estructurales e inmunohistoquímicos encontrados
Assuntos
Humanos , Feminino , Adulto , Diagnóstico Clínico , Tumor de Células da Granulosa/diagnóstico , Neoplasias Ovarianas/diagnóstico , Diagnóstico Diferencial , Tumor de Células da Granulosa/cirurgia , Tumor de Células da Granulosa/ultraestrutura , Imuno-Histoquímica , Queratinas , Neoplasias Ovarianas/ultraestrutura , Prognóstico , Procedimentos Cirúrgicos Operatórios , VimentinaRESUMO
Female, 5 years old; first pregnancy of term, eutocic, weight 3,100 g; breast fed, complete vaccination program. A bronchial pathology that was present seven months later, yielded with medical treatment. Her illness started on September, 1984 after sever trauma by horse kick, presenting with a tumor in left illiac fossa; there was pain, improved by analgesics; the tumor continued to grow up to 5 x 6 cm in diameter, painful on palpation, causing hospital admission. Thirty days later an ultrasonographic study reported an ovoid echogenic and echolucid mass of 6.5 cm in left ovary; an infra-umbilical laparotomy was performed, finding a left ovarian tumarration, ovoid, pink white, with vascular areas, 24 x 18 x 15 cm at maximal diameters, solid, smooth and bright; at section there was gray-white, nodular tissue, with cystic cavities, 0.5 cm. Electronic microscopy an immunochemistry study were carried out in order to confirm the diagnosis of juvenile granulosa cell tumor. The patient died seven months later.
Assuntos
Tumor de Células da Granulosa/ultraestrutura , Neoplasias Ovarianas/ultraestrutura , Pré-Escolar , Feminino , HumanosRESUMO
In 1977 Scully first described the juvenile granulosa cell tumor of the ovary (JGCT) as a special variation of the granulosa cells tumor, which occurs in the two first decades of life, and 97% of the cases show characteristic microscopic and histological features. Five previous cases have been reported concerning the ultramicroscopic characteristics of this ovarian neoplasia. The purpose of this paper is to report the immunohistochemical and ultrastructural characteristics in a case of OJCGT which occurred in a four year old girl with isosexual precocious pseudopuberty. The presence of vimentin and absence of keratin was proven immunohistochemically in this ovarian neoplasia. Intermediate filaments were found ultrastructurally. The combined use of immunohistochemical and ultrastructural techniques has proven to be of extraordinary usefulness for the differential diagnosis between epithelial and non epithelial ovarian tumors and adds a new and highly specific method to characterize and differentiate the cells of embryonic carcinoma, choriocarcinomas and endodermal sinus tumors which are keratin positive.