RESUMO
The presence of antibodies against human parainfluenza viruses type 2 and 3 was studied in 1 793 sera from an infantile population under 14 years old. The hemagglutination inhibition technique was used for screening the clinical samples. Of the total of analyzed sera, 1 382 (77.1%) were positive. The presence of antibodies against type 2 was confirmed in 320 of them (17.8%), and against type 3 in 334 (18.6%). The simultaneous seropositivity predominated in 805 (44.9%). The circulation of human parainfluenza viruses was corroborated during all the months of the year and in all the analyzed age groups. It was observed an increase in the positivity percentages with age.
Assuntos
Crupe/epidemiologia , Laringite/epidemiologia , Vírus da Parainfluenza 2 Humana/isolamento & purificação , Vírus da Parainfluenza 3 Humana/isolamento & purificação , Traqueíte/epidemiologia , Adolescente , Fatores Etários , Anticorpos Antivirais/análise , Criança , Pré-Escolar , Crupe/virologia , Cuba/epidemiologia , Testes de Inibição da Hemaglutinação , Humanos , Lactente , Recém-Nascido , Laringite/virologia , Vírus da Parainfluenza 2 Humana/imunologia , Vírus da Parainfluenza 3 Humana/imunologia , Vigilância da População , Estudos Retrospectivos , Estações do Ano , Estudos Soroepidemiológicos , Traqueíte/virologiaRESUMO
Samples from 14 free-ranging pampas deer (Ozotoceros bezoarticus celer) were collected in 1995 and 1998, at Campos del Tuyú Wildlife Reserve, Buenos Aires, Argentina. Hematology, serum chemistries, minerals and metals, and fecal parasites were analyzed. In addition, fecal ova and parasites were evaluated seasonally during 1998-2000. Serology for infectious diseases included blue-tongue, brucellosis, bovine respiratory syncytial virus infection, bovine viral diarrhea/mucosal disease, infectious bovine rhinotracheitis, Johne's disease (paratuberculosis), foot and mouth disease (FMD), leptospirosis (eight serovars), epizootic hemorrhagic disease, and parainfluenza-3 (PI-3). Three (21%) pampas deer had antibodies to Leptospira spp. and six (43%) to PI-3 virus. Serologic results for all other infectious agents were negative. Domestic cattle (n = 27) included in this study for comparison had antibodies to Leptospira, infectious bovine rhinotracheitis virus, bovine viral diarrhea virus, and PI-3 virus (74-100% of tested animals) and one animal (4%) to Brucella sp. All cattle had antibodies to FMD virus attributable to vaccination. This study provides the first data on the health status of the southernmost sub-species of pampas deer.
Assuntos
Bem-Estar do Animal , Doenças dos Bovinos/epidemiologia , Cervos/sangue , Leptospirose/veterinária , Vírus da Parainfluenza 3 Humana/imunologia , Infecções por Respirovirus/veterinária , Animais , Animais Selvagens , Anticorpos Antibacterianos/sangue , Anticorpos Antivirais/sangue , Argentina/epidemiologia , Análise Química do Sangue/veterinária , Bovinos , Fezes/parasitologia , Feminino , Leptospira/imunologia , Leptospirose/epidemiologia , Masculino , Infecções por Respirovirus/epidemiologia , Estações do Ano , Estudos SoroepidemiológicosAssuntos
Anticorpos Antivirais/sangue , Vírus da Parainfluenza 3 Humana/imunologia , Infecções por Paramyxoviridae/veterinária , Doenças dos Ovinos/epidemiologia , Animais , Chile/epidemiologia , Feminino , Testes de Inibição da Hemaglutinação , Infecções por Paramyxoviridae/epidemiologia , Prevalência , OvinosAssuntos
Anticorpos Antibacterianos/análise , Anticorpos Antivirais/análise , Infecções Bacterianas/veterinária , Doenças das Cabras/epidemiologia , Viroses/veterinária , Animais , Infecções Bacterianas/epidemiologia , Vírus Bluetongue/imunologia , Brasil/epidemiologia , Chlamydophila psittaci/imunologia , Corynebacterium/imunologia , Coxiella/imunologia , Vírus da Diarreia Viral Bovina/imunologia , Feminino , Cabras , Herpesviridae/imunologia , Masculino , Vírus da Parainfluenza 3 Humana/imunologia , Prevalência , Estudos Soroepidemiológicos , Viroses/epidemiologiaRESUMO
An inactivated vaccine was prepared with Parainfluenza-3 virus strain LQ-514 and strains of Pasteurella hemolytica and P. multocida, suspended in oil adjuvant. The virus had been isolated from 30-60 day old calves during an epidemic of Pneumonia. The vaccine was tested in guinea pigs aged 1 to 2 months. The antibody response and the virus titres in organs after the challenge were the parameters studied. Hemagglutination inhibition antibodies were first detected 14 days after vaccination and reached maximum titres at day 28. The challenge was done at day 34, and a secondary antibody response was observed 72 hours later, which reached its peak the following day. Virus could be isolated from lung samples of control animals at day 3, 4 and 5 after infection. Moreover, viral antigens and particles were observed in the same samples by immunofluorescence and electron microscopy, respectively. In contrast, all three methods failed to demonstrate the presence of virus in organs of immunized guinea pigs after the challenge.
Assuntos
Vírus da Parainfluenza 3 Humana/imunologia , Respirovirus/imunologia , Vacinas Virais/imunologia , Animais , Anticorpos Antivirais/análise , Antígenos Virais/análise , Feminino , Cobaias , Pulmão/microbiologia , Vírus da Parainfluenza 3 Humana/isolamento & purificação , VacinaçãoRESUMO
Se elaboró una vacuna inactivada con la cepa LQ-514 del virus Parainfluenza-3 aislado en brotes de neumonía en terneros de 30 a 60 días de edad y cepas de Pasteurella hemolítica y P. multocida. En este trabajo se estudió la posibilidad de evaluar dicha vacuna con adyuvante oleoso en cobayos de 1 a 2 meses de edad, a través de la respuesta de anticuerpos y aislamiento de virus luego de la descarga. En los animales vacunados pudieron detectarse anticuerpos inhibidores de la hemoaglutinación a partir del día 14 p.i. llegando a títulos máximos el día 28 p.i. A los tres días de la descarga se evidenció una respuesta de tipo secundario en los animales vacunados, alcanzando el máximo al cuarto día. El virus pudo aislarse en cultivos celulares a partir de pulmón de animales no inmunizados los días 3,4 y 5 post descarga, evidenciándose además su presencia por inmunofluorescencia directa y por microscopía electrónica. Por el contrario, en los animales vacunados y descargados no pudo aislarse virus de pulmón ni ponerse en evidencia por inmunofluorescencia directa o microscopía electrónica
Assuntos
Cobaias , Animais , Formação de Anticorpos , Vírus da Parainfluenza 3 Humana/imunologia , Vacinas Atenuadas/imunologia , Imunização , Vírus da Parainfluenza 3 Humana/isolamento & purificação , Pulmão/microbiologiaRESUMO
Se elaboró una vacuna inactivada con la cepa LQ-514 del virus Parainfluenza-3 aislado en brotes de neumonía en terneros de 30 a 60 días de edad y cepas de Pasteurella hemolítica y P. multocida. En este trabajo se estudió la posibilidad de evaluar dicha vacuna con adyuvante oleoso en cobayos de 1 a 2 meses de edad, a través de la respuesta de anticuerpos y aislamiento de virus luego de la descarga. En los animales vacunados pudieron detectarse anticuerpos inhibidores de la hemoaglutinación a partir del día 14 p.i. llegando a títulos máximos el día 28 p.i. A los tres días de la descarga se evidenció una respuesta de tipo secundario en los animales vacunados, alcanzando el máximo al cuarto día. El virus pudo aislarse en cultivos celulares a partir de pulmón de animales no inmunizados los días 3,4 y 5 post descarga, evidenciándose además su presencia por inmunofluorescencia directa y por microscopía electrónica. Por el contrario, en los animales vacunados y descargados no pudo aislarse virus de pulmón ni ponerse en evidencia por inmunofluorescencia directa o microscopía electrónica (AU)
Assuntos
Cobaias , Animais , Formação de Anticorpos , Vacinas Atenuadas/imunologia , Vírus da Parainfluenza 3 Humana/imunologia , Imunização , Pulmão/microbiologia , Vírus da Parainfluenza 3 Humana/isolamento & purificaçãoAssuntos
Anticorpos Antivirais/análise , Bovinos/imunologia , Indústria de Laticínios , Vírus da Diarreia Viral Bovina/imunologia , Herpesvirus Bovino 1/imunologia , Vírus da Leucemia Bovina/imunologia , Vírus da Parainfluenza 3 Humana/imunologia , Pestivirus/imunologia , Respirovirus/imunologia , Retroviridae/imunologia , Animais , Colômbia , FemininoAssuntos
Anticorpos Antivirais/análise , Bovinos/imunologia , Adenoviridae/imunologia , Animais , Vírus Bluetongue/imunologia , Vírus da Diarreia Viral Bovina/imunologia , Herpesviridae/imunologia , Vírus da Leucemia Bovina/imunologia , México , Vírus da Parainfluenza 3 Humana/imunologia , Rotavirus/imunologiaRESUMO
In order to determine the natural history of parainfluenza virus infection in early life, we followed prospectively 130 infants and children from birth or a few months of age for evidence of infection with PV. Using rapid diagnostic techniques, standard tissue culture infectivity, and serologic techniques we were able to document primary PV infection in 92% of these infants, and repeated infection with heterotypic or homotypic PV strains in 49% by 30 months of age. Increasing patient age had no significant effect in reducing the incidence of lower respiratory tract illness as a result of PV infection. Infection with one PV serotype provided no protection against LRTI at the time of subsequent infection with a heterotypic PV strain. In contrast, primary PV infection provided a brief period of immunity to LRTI upon homotypic reinfection. Secretory IgA responses to PV were determined by immunofluorescent techniques. Antibody response to PV strains causing primary infection and heterotypic repeated infection were transient and of low magnitude. Homotypic reinfection resulted in significantly enhanced production of secretory antibody to PV. At least in early life, repeated exposures to PV appear to be essential for maintaining immunity to severe forms of illness caused by PV infection.