RESUMO
In this study, we aimed to characterize the distribution of Yersinia enterocolitica in a pork production chain in Brazil, as well as the virulence profile and antibiotic resistance of the obtained isolates. Samples from 10 pig lots obtained from finishing farms (water, feed, and barn floors, n = 30), slaughterhouse (lairage floors, carcasses at four processing steps, tonsils, and mesenteric lymph nodes, n = 610), and processing (end cuts, processing environment, n = 160) were obtained in Paraná state, Brazil, and subjected to Y. enterocolitica detection by ISO 10,273. The obtained isolates were identified based on biochemical and molecular features (16 s rRNA, inv, bioserotyping) and subjected to PCR assays to detect virulence (ail, ystA, ystB, virF, myfA, fepA, fepD, fes, tccC, ymoA, hreP, and sat) and multidrug resistance-related genes (emrD, yfhD, and marC). Also, isolates were subjected to disk diffusion test to characterize their resistance against 17 antibiotics from 11 classes and to pulsed field gel electrophoresis (PFGE) after XbaI macro-restriction. Y. enterocolitica was detected in a single sample (tonsil), and the obtained three isolates were characterized as serotype O:3, harboring ail, ystA, virF, myfA, tccC, ymoA, hreP, emrD, yfhD, and marC, and resistant to all tested antibiotics. The three isolates presented identical macro-restriction profiles by PFGE, also identical to isolates obtained from Minas Gerais, other Brazilian state; one selected isolate was identified as biotype 4. Despite the low occurrence of Y. enterocolitica in the studied pork production, the virulence potential and the antibiotic resistance profiles of the isolates demonstrated their pathogenic potential, and the macro-restriction profiles indicate strains descending from a common subtype in the pork production chain of two Brazilian States.
Assuntos
Doenças Transmitidas por Alimentos , Carne de Porco , Yersiniose , Yersinia enterocolitica , Animais , Antibacterianos/farmacologia , Brasil , Resistência Microbiana a Medicamentos/genética , Doenças Transmitidas por Alimentos/microbiologia , Tonsila Palatina/microbiologia , Carne de Porco/microbiologia , Suínos , Doenças dos Suínos/microbiologia , Yersiniose/microbiologia , Yersiniose/transmissão , Yersinia enterocolitica/efeitos dos fármacos , Yersinia enterocolitica/genética , Yersinia enterocolitica/patogenicidadeAssuntos
Microbiologia de Alimentos , Doenças Transmitidas por Alimentos/etiologia , Carne/efeitos adversos , Estações do Ano , Yersiniose/transmissão , Yersinia enterocolitica , Animais , Manipulação de Alimentos , Doenças Transmitidas por Alimentos/microbiologia , Humanos , Lactente , Intestinos/microbiologia , Masculino , Carne/microbiologia , Suínos , Yersiniose/microbiologiaRESUMO
A search for Y. enterocolitica in fresh foods of animal origin has been carried out. Isolations were obtained from "chorizos" (thick fresh sausages), "salchichas" (slender fresh sausages), bovine and pig's tongues and caecum (Tables 1, 2). Enrichments were performed in saline phosphate buffer 0.067 M, pH 7.6 and nutrient broth with 0.5% glucose. Caecum and tongue samples were postenriched in 0.5% KOH. Subcultures were done in Salmonella-Shigella agar, MacConkey agar and eosin-methylene blue agar. Isolates were identified through biochemical, serological and lysotyping methods. The following biotypes (B), serotypes (O) and lysotypes (Lis) were isolated: from "chorizos", B2, 0:9, Lis X3 and B1, 0:7,8, Lis Xo. From "salchichas", B1, 0:5, Lis Xz. From bovine tongues, B1, 0:5, Lis Xz and B2, 0:9, Lis X3. From pig tongues, B1, 0:6, Lis Xz. From pig caecum, B1, 0:6, Lis Xz and B1, 0:5, Lis Xz. Serotype B2, 0:9, Lis X3 isolated from "chorizos" and bovine tongues has been only exceptionally found outside of human beings. Serotype B1, 0:6, Liz Xz was isolated from the tongue and caecum of one pig. From the caecum of another pig two serotypes, B1, 0:5, Liz Xz and B1, 0:6, Liz Xz, were isolated. In order to determine the importance of Y. entercolitica as etiologic agent of yersiniosis in Argentina, further studies on the frequency and distribution of this agent are recommended.