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We investigated the effects of corn grain, finely or coarsely ground, rehydrated and ensiled to 35% moisture in substitution of dry corn grain on performance of beef cattle in the feedlot. Forty non-castrated young Angus crossbred bulls with average age of 13±1.4 months and average initial body weight (BW) of 374±14 kg. The experiment was conducted in blocks by weight, and bulls were randomly assigned into four groups of five animals each in a 2 × 2 factorial scheme. The factors evaluated were particle size (finely and coarsely ground) and two grain sources (dry ground corn and rehydrated corn grain silage). The treatments were diets containing dry corn grain, finely ground (DCF; 1.86 mm); dry corn grain, coarsely ground (DCC; 3.53 mm); rehydrated and ensiled corn grain, finely ground (RCF; 1.86 mm); and rehydrated and ensiled corn grain, coarsely ground (RCC; 3.53 mm). Initial BW, final BW, average daily gain (ADG), feed efficiency, and intake of dry matter (DMI), acid detergent fiber, and metabolizable energy were not affected by treatment. Ensiling corn grain decreased DMI by 10.3% (11.6 vs. 10.4 kg/d for dry and ensiled, respectively) and increased feed efficiency by 13.3% (0.13 vs. 0.15 kg/d for dry and ensiled, respectively), but there was no effect of particle size, grain source, and their interaction on ADG. Effects of particle size and grain source were observed for fecal starch and total tract starch digestion, with evidence that treatments containing rehydrated corn diets showed greater efficiency in the utilization of dietary starch. Animals fed RCF diets showed lower fecal starch losses of 37, 55, and 75% when compared with treatments RCC, DCF, and DCC, respectively. Our results suggested that ensiled rehydrated corn grain improves feed efficiency in substitution of dry corn grain. The finely and coarsely ground of rehydrated and ensiled corn grain increases the digestibility of starch for finishing cattle in feedlot.(AU)

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This study aimed to evaluate the effects of ground or steam-flaked corn and zinc-enriched yeast addition to grower pellet feed on fattening performance, rumen papillae development, and some blood parameters in lambs. For this purpose, thirty-six Kivircik male lambs were selected and divided equally into six groups: basal diet containing pellet feed without different corn form and yeast (control), basal diet 80% + ground corn 20% (PGC), basal diet 80% + steam-flaked corn 20% (PFC), PGC + Zn-enriched yeast (PGCZnY), PFC + Zn-enriched yeast (PFCZnY), and control + Zn-enriched yeast (PZnY). They were fed for 56 days according to the diets mentioned above. At the conclusion of the evaluations in fattening performance, rumen papillae development, and some blood parameters, there were no significant differences in body weight gain, average daily gain, average daily feed intake, and feed efficiency among the experimental groups. In the PGCZnY group, rumen papillae length was found to be higher than the other experimental groups. At the end of the trial (day 0), leukocyte and lymphocyte counts decreased significantly only in the control group compared with the other groups in the present study. Serum blood urea nitrogen (BUN) values, analyzed at the beginning and at the end of the trial, increased significantly in all groups except in the PGCZnY group. Supplementation of Zinc-enriched yeast to ground corn can be used for increasing ruminal papilla length, however decreasing the serum BUN levels in lambs during the fattening period.(AU)

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This study aimed at assessing the effect of pelleted and expanded maize-based feeds prepared with different moisture levels (ML, 0.8 and 1.6%) particle size (PS, 650 and 850µ) of ingredients on metabolizable energy, ileal digestibility of amino acids and broiler performance. A total of 720 one-day-old male broiler chicks of the Cobb strain were used. The experiment was performed over a period of 40d (days) and birds received water and feed ad libitum. From 10 to 13d higher AMEn values were obtained for birds fed expanded feeds with 1.6% ML and 850µ PS. Higher values AMEn were encountered when adding 0.8% ML in pellet feeds with 850µ or 1.6%ML/650µ PS. Expanded and pelleted diets with 1.6% ML (independent of PS, 650 or 850µ) presented higher amino acid digestibility. For diets with 0.8% of ML, better result for the digestibility of amino acids were obtained with PS 650µ for expanded and 850µ for pelleted diets. Higher weight gain was observed in broilers fed diets with 1.6% moisture at 21 and 40d. Feed conversion was improved by adding 1.6% ML only at 21d. Regarding carcass characteristics, expanded diets combined with PS of 650µ led to a higher accumulation of abdominal fat. The birds fed expanded diets at 1.6% moisture had higher breast yields. We concluded that to increase the use of nutrients, EMAn and broiler performance, it is recommended that pelleted diets be prepared with the addition of 1.6% water to the mixer and a particle size of 850µ.(AU)

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The objective of the present study was to compare the effects of corn processing on performance and intestinal parameters of weanling piglets. To accomplish our goal, 42 piglets (21 days-old, 7.18±1.0 kg body weight) were randomly allocated (seven pens/treatment; three pigs/pen) to one of two treatments: ground corn ­ corn ground through a hammer mill with a 1.0-mm screen after being ground by a hammer mill with a 3.0-mm screen, and extruded corn ­ corn was wet extruded after being ground by a hammer mill with a 2.0-mm screen and, after extrusion, it was ground by a hammer mill with a 1.0-mm screen. In both methods, corn from the same batch was used. Results were considered statistically significant when P<0.05 and a tendency when P<0.10. Analyses were performed using the GLIMMIX procedure of SAS. Pigs fed ground or extruded corn diet had similar growth performance. Duodenum and jejunum of pigs fed extruded corn had greater villus height compared with those of pigs fed ground corn. Crypt depth was not influenced by corn processing. Duodenum and jejunum of pigs fed extruded corn had greater villus:crypt ratio compared with those of pigs fed ground corn. In the duodenum, the expression of zonula occludens-1 (ZO-1) was greater, and the expression of Occludin-1 tended to be greater in pigs fed the extruded corn diets. There was no effect of corn processing on ZO-1 and Occludin-1 expression in the jejunum, nor TGF-ß1 expression in duodenum and jejunum. There was no effect of the type of corn processing on colonic total bacteria or Enterobacteriaceae and Lactobacillus abundance. Piglets fed diets with ground corn or extruded corn have similar growth performance. However, piglets fed diets with extruded corn present improved intestinal morphology and tight junction protein expression compared with those fed ground corn.(AU)

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The objective of this study was to evaluate the effect of replacing ground corn (402 g kg−1 of dry matter) with rehydrated corn grain silage (RCGS; 425 g kg−1 of dry matter) in a flushing diet on follicular development in tropical Santa Inês ewes. Fifteen ewes were randomly assigned to one of two treatment groups: a diet with ground corn (control, n = 7) or a diet with RCGS (n = 8). The first day of the diets was designated d0, and the diets were fed for 30 days, up to two days after the end of the estrus synchronization protocol. The estrus synchronization protocol (intravaginal progestogen sponge for 11 days plus 300 IU eCG and 37.5 µg d-cloprostenol IM two days before sponge removal) started on d17 of the diets. The number and diameters of ovarian follicles ≥3 mm were assessed by ultrasound on the day before the diets were provided (d−1), on d14, and then daily from two days before sponge removal until ovulation or up to the eighth day after sponge removal. Blood samples were collected on days −1, 0, 6, 12, 18, 24, and 30 for glucose and urea analyses. There was no effect of the treatments on dry matter intake, plasma glucose, and urea nitrogen concentrations, or on the percentage of ewes that ovulated and on the number of ovulations. The number of follicles ≥3 mm did not differ between treatment groups; the number increased between d−1 and d14 and did not differ between d14 and d28. The diameter of ovulatory follicles at sponge removal, the interval from sponge removal to estrus, and the growth rate of the ovulatory follicles were greater in the RCGS group than in the control group, resulting in larger follicles at ovulation. Replacement of ground corn by RCGS in the flushing diet does not increase the number of ovulations but results in delayed onset of estrus and ovulation of larger follicles.(AU)

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A large outbreak of botulism in feedlot steers fed corn silage contaminated with Clostridium botulinum neurotoxin type C (BoNT/C) is reported occurring in Midwestern Brazil in August 2017. The onset of the outbreak occurred 15 days after 1700 steers started to be fed the contaminated corn silage. Affected steers were alert and afebrile with varying degrees of flaccid paralysis in various muscle groups. A total of 1100 steers were affected, 1090 of which died within four days. Ten steers recovered after treatment with antitoxin. No gross or microscopic lesions were found in affected steers. The diagnosis was based on epidemiological data, characteristic clinical signs, and positive mouse bioassay results. This outbreak is interesting due to the high number of fatally affected cattle and the on-site diagnostic approach. This case report demonstrates the difficulties in diagnosing and treating botulism in cattle.

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The present study aimed to evaluate non-fiber carbohydrates (NFC) in sugarcane-based diets on rumen pH, and forage digestibility, and to describe NFC degradation curves. The study consisted of two trials. For the first trial, three rumen cannulated steers, BW of 350 ± 15 kg (mean ± SE), were assigned in a 3×3 Latin Square (LS) design. They were fed diets containing finely-ground (0.9 mm average particle size) corn (GC), steam-rolled corn (SRC), or pelleted citrus pulp (PCP). Each period had 14 d, with the first 12 for adaptation. The 13th d was for serial measurement of rumen pH, and the14th for rumen fluid collection and in vitro incubation for DM and NDF digestibility (IVDMD and IVNDFD) of bermudagrass hay (Hay), corn (CS), and sugarcane (SS) silages. In the second trial, rumen fluid of a cannulated bull, fed corn silage and a regular concentrate, was collected for in vitro digestion of NFC for multiple time points. The incubation results were used to adjust the NFC degradation curves, and calculate lag-time, feed fractions, and degradation rate. Data from first trial was analyzed in a 3×3 LS. The model for the digestibility parameters included fixed effects of forage (Feed), diets with NFC (Diet), and their interaction (Feed × Diet), and random effect of animal and period. The model for rumen pH included fixed effect of diet, time as repeated measures, animal and period as random effects. The significance was considered at probability ≤ 5% (α = 0.05). The NFC degradation curves were adjusted using the PROC NLIN procedure from SAS, and equation parameters compared using confidence intervals. There was a Diet × Time interaction on rumen pH (P = 0.04), where SRC decreased pH compared to PCP and GC diets at the time 6 h, only. There was no Feed × Diet interaction effect (P > 0.05) for any digestibility parameter. There was a Feed effect on both IVDMD and IVNDFD, either after 30 or 48 h incubation (P < 0.01). The CS had the greatest IVDMD, followed by SS and Hay, after 30 and 48 h of incubation. The CS had the greatest IVNDFD after 30 h, compared to SS and Hay. However, for IVNDFD after 48 h, CS presented the greatest mean, followed by SS and Hay. The rumen fluid from animals fed SRC decreased both IVDMD and IVNDFD (P < 0.05) of all roughages after 48 h. Results from the second trial showed that the PCP had lower Lag Time, B fraction and greater kd compared to both corn sources, and SRC had greater kd than GC. In conclusion, the SRC diet decreased rumen pH 6 h after feeding and, consequently, decreased fiber digestibility of the tropical forage sources evaluated. Although the PCP had lower lag time, and faster rate of degradation of B fraction, it did not negatively affect rumen pH or fiber digestibility of forage.(AU)

O presente estudo teve como objetivo avaliar os carboidratos não-fibrosos (CNF) em dietas à base de cana-de-açúcar sobre o pH ruminal e digestibilidade da forragem, e descrever as curvas de degradação dos CNF. O estudo foi composto de dois ensaios. No primeiro, três novilhos canulados no rúmen, com peso vivo de 350 ± 15 kg (Média ± DP), foram alocados em um quadrado latino (QL) 3×3, e alimentados com dietas contendo: milho moído (MM, tamanho de partículas 0,9 mm), laminado a vapor (MLV) ou polpa cítrica peletizada (PCP). Cada período tinha 14 d, sendo os primeiros 12 para adaptação e o 13º para a medição seriada do pH e o 14º para a coleta de líquido ruminal e incubação in vitro para digestibilidade da MS e FDN (DIVMS e DIVFDN) de feno de bermudagrass (Feno) e silagens de milho (SM) e cana (SC). No segundo ensaio, coletou-se fluido ruminal de um touro canulado, alimentado com silagem de milho e concentrado padrão, para digestão in vitro dos CNF em vários tempos. Esses resultados foram utilizados para ajustar as curvas de degradação dos CNF e calcular o tempo de colonização, frações alimentares e taxa de degradação. Os resultados do primeiro ensaio foram analisados em um QL 3×3. O modelo dos parâmetros de digestibilidade incluiu efeito fixo de forragem (Alimento), dieta com CNF (Dieta) e interação (Alimento × Dieta), e efeito aleatório de animal e período. O modelo para pH incluiu efeito fixo de Dieta, Tempo como medida repetida, animal e período como aleatórios. Foi considerada a probabilidade significativa de ≤ 5% (α = 0,05). As curvas de degradação dos CNF foram ajustadas pelo PROC NLIN do SAS, e parâmetros de equação comparados por intervalo de confiança. Houve interação Dieta × Tempo no pH ruminal (P = 0,04), onde o MLV diminuiu o pH comparado com PCP e MM apenas no tempo 6 h. Não houve interação Alimento × Dieta (P > 0,05) para nenhum parâmetro de digestibilidade. Houve efeito de Alimento sobre a DIVMS e DIVFDN, após 30 e 48 h de incubação (P < 0,01). A SM teve a maior DIVMS, seguido por SC e Feno, após 30 e 48 h de incubação. A SM teve a maior DIVFDN após 30 h, comparado com SC e Feno. No entanto, para DIVFDN após 48 h, a SM teve maior média, seguida da SC e Feno. O fluido ruminal de animais alimentados com MLV diminuiu a DIVMS e DIVFDN (P < 0.05) de todas as forragens, após 48 h. Resultados do segundo ensaio mostram que PCP diminuiu o tempo de colonização, fração B e aumentou a kd comparado com os dois milhos, e MLV apresentou maior kd que o MM. Em conclusão, a dieta com MLV diminuiu o pH ruminal no tempo 6 h e, consequentemente, diminuiu a DIVFDN das forragens avaliadas. Embora PCP tenha apresentado menor tempo de colonização e maior taxa de degradação da fração B, não afetou negativamente o pH do rúmen nem a digestibilidade da fibra das forragens. (au)

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RESUMO

The present study aimed to evaluate non-fiber carbohydrates (NFC) in sugarcane-based diets on rumen pH, and forage digestibility, and to describe NFC degradation curves. The study consisted of two trials. For the first trial, three rumen cannulated steers, BW of 350 ± 15 kg (mean ± SE), were assigned in a 3×3 Latin Square (LS) design. They were fed diets containing finely-ground (0.9 mm average particle size) corn (GC), steam-rolled corn (SRC), or pelleted citrus pulp (PCP). Each period had 14 d, with the first 12 for adaptation. The 13th d was for serial measurement of rumen pH, and the14th for rumen fluid collection and in vitro incubation for DM and NDF digestibility (IVDMD and IVNDFD) of bermudagrass hay (Hay), corn (CS), and sugarcane (SS) silages. In the second trial, rumen fluid of a cannulated bull, fed corn silage and a regular concentrate, was collected for in vitro digestion of NFC for multiple time points. The incubation results were used to adjust the NFC degradation curves, and calculate lag-time, feed fractions, and degradation rate. Data from first trial was analyzed in a 3×3 LS. The model for the digestibility parameters included fixed effects of forage (Feed), diets with NFC (Diet), and their interaction (Feed × Diet), and random effect of animal and period. The model for rumen pH included fixed effect of diet, time as repeated measures, animal and period as random effects. The significance was considered at probability ≤ 5% (α = 0.05). The NFC degradation curves were adjusted using the PROC NLIN procedure from SAS, and equation parameters compared using confidence intervals. There was a Diet × Time interaction on rumen pH (P = 0.04), where SRC decreased pH compared to PCP and GC diets at the time 6 h, only. There was no Feed × Diet interaction effect (P > 0.05) for any digestibility parameter. There was a Feed effect on both IVDMD and IVNDFD, either after 30 or 48 h incubation (P < 0.01). The CS had the greatest IVDMD, followed by SS and Hay, after 30 and 48 h of incubation. The CS had the greatest IVNDFD after 30 h, compared to SS and Hay. However, for IVNDFD after 48 h, CS presented the greatest mean, followed by SS and Hay. The rumen fluid from animals fed SRC decreased both IVDMD and IVNDFD (P < 0.05) of all roughages after 48 h. Results from the second trial showed that the PCP had lower Lag Time, B fraction and greater kd compared to both corn sources, and SRC had greater kd than GC. In conclusion, the SRC diet decreased rumen pH 6 h after feeding and, consequently, decreased fiber digestibility of the tropical forage sources evaluated. Although the PCP had lower lag time, and faster rate of degradation of B fraction, it did not negatively affect rumen pH or fiber digestibility of forage.(AU)

O presente estudo teve como objetivo avaliar os carboidratos não-fibrosos (CNF) em dietas à base de cana-de-açúcar sobre o pH ruminal e digestibilidade da forragem, e descrever as curvas de degradação dos CNF. O estudo foi composto de dois ensaios. No primeiro, três novilhos canulados no rúmen, com peso vivo de 350 ± 15 kg (Média ± DP), foram alocados em um quadrado latino (QL) 3×3, e alimentados com dietas contendo: milho moído (MM, tamanho de partículas 0,9 mm), laminado a vapor (MLV) ou polpa cítrica peletizada (PCP). Cada período tinha 14 d, sendo os primeiros 12 para adaptação e o 13º para a medição seriada do pH e o 14º para a coleta de líquido ruminal e incubação in vitro para digestibilidade da MS e FDN (DIVMS e DIVFDN) de feno de bermudagrass (Feno) e silagens de milho (SM) e cana (SC). No segundo ensaio, coletou-se fluido ruminal de um touro canulado, alimentado com silagem de milho e concentrado padrão, para digestão in vitro dos CNF em vários tempos. Esses resultados foram utilizados para ajustar as curvas de degradação dos CNF e calcular o tempo de colonização, frações alimentares e taxa de degradação. Os resultados do primeiro ensaio foram analisados em um QL 3×3. O modelo dos parâmetros de digestibilidade incluiu efeito fixo de forragem (Alimento), dieta com CNF (Dieta) e interação (Alimento × Dieta), e efeito aleatório de animal e período. O modelo para pH incluiu efeito fixo de Dieta, Tempo como medida repetida, animal e período como aleatórios. Foi considerada a probabilidade significativa de ≤ 5% (α = 0,05). As curvas de degradação dos CNF foram ajustadas pelo PROC NLIN do SAS, e parâmetros de equação comparados por intervalo de confiança. Houve interação Dieta × Tempo no pH ruminal (P = 0,04), onde o MLV diminuiu o pH comparado com PCP e MM apenas no tempo 6 h. Não houve interação Alimento × Dieta (P > 0,05) para nenhum parâmetro de digestibilidade. Houve efeito de Alimento sobre a DIVMS e DIVFDN, após 30 e 48 h de incubação (P < 0,01). A SM teve a maior DIVMS, seguido por SC e Feno, após 30 e 48 h de incubação. A SM teve a maior DIVFDN após 30 h, comparado com SC e Feno. No entanto, para DIVFDN após 48 h, a SM teve maior média, seguida da SC e Feno. O fluido ruminal de animais alimentados com MLV diminuiu a DIVMS e DIVFDN (P < 0.05) de todas as forragens, após 48 h. Resultados do segundo ensaio mostram que PCP diminuiu o tempo de colonização, fração B e aumentou a kd comparado com os dois milhos, e MLV apresentou maior kd que o MM. Em conclusão, a dieta com MLV diminuiu o pH ruminal no tempo 6 h e, consequentemente, diminuiu a DIVFDN das forragens avaliadas. Embora PCP tenha apresentado menor tempo de colonização e maior taxa de degradação da fração B, não afetou negativamente o pH do rúmen nem a digestibilidade da fibra das forragens. (au)

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The effect on tambaqui fingerlings performance caused by diets containing two different carbohydrate sources was evaluated. Four isoproteic diets with different mango meal concentrations (0, 33, 66 and 100%) replacing cornmeal were tested. The feeding trial lasted 45 days and the feeding rate was 8% of total biomass. 240 tambaqui fingerlings with an initial weight average of 3.66g were distributed in 12 500-liter tanks in a recirculating water system with biofilter. Performance, feed intake, carcass yield, survival, total cholesterol, plasmatic free amino acids, hepatic aspartate aminotransferase(AST), hepatic glycogen and plasma glucose values were evaluated. The results showed that weight gain, daily weight gain, specific growth rate, apparent food consumption, cholesterol, AST and glycogen showed higher values in the treatments with the highest levels of mango meal. Apparent feed conversion, carcass yield, survival and plasma glucose were not affected by the two sources of carbohydrates tested. In conclusion, mango meal used in a practical diet for tambaqui improved growth performance.

Avaliou-se o efeito sobre o desempenho de alevinos de tambaqui alimentados com dietas contendo duas fontes diferentes de carboidratos. Foram testadas quatro dietas isoprotéicas com diferentes concentrações de farinha de manga (0, 33, 66 e 100%) em substituição ao farelo de milho. O teste de alimentação foi de 45 dias e a taxa de alimentação foi de 8% da biomassa total. Foram utilizados 240 alevinos de tambaqui com peso inicial médio de 3,66g distribuídos em 12 caixas de 500L em sistema de recirculação de água com biofiltro. Foram avaliados os índices de desempenho, consumo de ração, rendimento de carcaça, sobrevivência, colesterol total, aminoácidos livres plasmáticos, aspartato aminotransferase hepática (AST), glicogênio hepático e glicose plasmática. Os resultados mostraram que o ganho ponderal de peso, a taxa de crescimento específico, o consumo aparente de dieta, colesterol, AST e glicogênio apresentaram valores maiores nos tratamentos com os maiores níveis de farinha de manga. A conversão alimentar aparente, o rendimento de carcaça, a sobrevivência e a glicose plasmática não foram afetados pelas duas fontes de hidratos de carbono testadas. Em conclusão, a farinha de manga utilizado na dieta prática para tambaqui melhorou o desempenho.

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RESUMO

The effect on tambaqui fingerlings performance caused by diets containing two different carbohydrate sources was evaluated. Four isoproteic diets with different mango meal concentrations (0, 33, 66 and 100%) replacing cornmeal were tested. The feeding trial lasted 45 days and the feeding rate was 8% of total biomass. 240 tambaqui fingerlings with an initial weight average of 3.66g were distributed in 12 500-liter tanks in a recirculating water system with biofilter. Performance, feed intake, carcass yield, survival, total cholesterol, plasmatic free amino acids, hepatic aspartate aminotransferase(AST), hepatic glycogen and plasma glucose values were evaluated. The results showed that weight gain, daily weight gain, specific growth rate, apparent food consumption, cholesterol, AST and glycogen showed higher values in the treatments with the highest levels of mango meal. Apparent feed conversion, carcass yield, survival and plasma glucose were not affected by the two sources of carbohydrates tested. In conclusion, mango meal used in a practical diet for tambaqui improved growth performance.(AU)

Avaliou-se o efeito sobre o desempenho de alevinos de tambaqui alimentados com dietas contendo duas fontes diferentes de carboidratos. Foram testadas quatro dietas isoprotéicas com diferentes concentrações de farinha de manga (0, 33, 66 e 100%) em substituição ao farelo de milho. O teste de alimentação foi de 45 dias e a taxa de alimentação foi de 8% da biomassa total. Foram utilizados 240 alevinos de tambaqui com peso inicial médio de 3,66g distribuídos em 12 caixas de 500L em sistema de recirculação de água com biofiltro. Foram avaliados os índices de desempenho, consumo de ração, rendimento de carcaça, sobrevivência, colesterol total, aminoácidos livres plasmáticos, aspartato aminotransferase hepática (AST), glicogênio hepático e glicose plasmática. Os resultados mostraram que o ganho ponderal de peso, a taxa de crescimento específico, o consumo aparente de dieta, colesterol, AST e glicogênio apresentaram valores maiores nos tratamentos com os maiores níveis de farinha de manga. A conversão alimentar aparente, o rendimento de carcaça, a sobrevivência e a glicose plasmática não foram afetados pelas duas fontes de hidratos de carbono testadas. Em conclusão, a farinha de manga utilizado na dieta prática para tambaqui melhorou o desempenho.(AU)

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