RESUMO
There are numerous poisonous plants that can induce intralysosomal accumulation of glycoproteins and neurologic syndromes. Here we describe for the first time, a disease caused by ingesting Sida rodrigoi Monteiro in goats in North-western Argentina. The animals showed weight loss, indifference to the environment, unsteady gait and ataxia. Histopathologic studies showed vacuolization in cells of various organs, mainly in the CNS. The material deposited in the cells was positive for LCA (Lens culinaris agglutinin), WGA (Triticum vulgaris agglutinin), sWGA (succinyl-Triticum vulgaris agglutinin) and Con-A (Concanavalia ensiformis agglutinin) lectins. Finally, toxic levels of swansonine were identified in the plant. The present investigation allowed to recognize S. rodrigoi Monteiro poisoning as a plant induced α-mannosidosis.
Assuntos
Doenças das Cabras/diagnóstico , Malvaceae/química , Intoxicação por Plantas/veterinária , Swainsonina/toxicidade , alfa-Manosidose/veterinária , Ração Animal/análise , Animais , Argentina , Ataxia/diagnóstico , Ataxia/etiologia , Ataxia/veterinária , Sistema Nervoso Central/fisiopatologia , Dieta/veterinária , Doenças das Cabras/etiologia , Cabras , Lectinas de Plantas/análise , Intoxicação por Plantas/diagnóstico , Intoxicação por Plantas/etiologia , Plantas Tóxicas/química , alfa-Manosidose/diagnóstico , alfa-Manosidose/etiologiaRESUMO
A disease of the nervous system is reported in goats in the semiarid region of northeastern Brazil. Histological examination showed diffuse vacuolation of neurons and epithelial cells of the pancreas, thyroid, renal tubules, and liver. The swainsonine-containing plant Ipomoea verbascoidea was found on both farms where the goats originated. This plant was experimentally administered to 3 goats, inducing clinical signs and histologic lesions similar to those observed in spontaneous cases. On the lectin histochemical analysis, cerebellar cells and pancreatic acinar cells gave positive reactions to Triticum vulgaris agglutinin (WGA), succinylated Triticum vulgaris agglutinin (sWGA), Lens culinaris agglutinin (LCA), Canavalia ensiformis agglutinin (ConA), Pisum sativum agglutinin (PSA), Ricinus communis agglutinin (RCA(120)), Arachis hypogaea agglutinin (PNA), and Phaseolus vulgaris erythroagglutinin (PHA-E) suggesting storage of α-fucose, α-D-mannose, α-D-glucose, ß-D-N-acetyl-glucosamine, N-acetyl-galactosamine, and acetyl-neuraminic acid. This pattern of lectin staining partially agrees with results previously reported for poisoning by swainsonine-containing plants. The chemical analysis of dried leaves of I. verbascoidea detected swainsonine (0.017%), calystegine B(1) (0.16%), calystegine B(2) (0.05%), and calystegine C(1) (0.34%). It is concluded that I. verbascoidea causes α-mannosidosis in goats.
Assuntos
Doenças das Cabras/etiologia , Ipomoea/efeitos adversos , Intoxicação por Plantas/veterinária , Swainsonina/efeitos adversos , alfa-Manosidose/veterinária , Animais , Brasil , Doenças das Cabras/diagnóstico , Doenças das Cabras/patologia , Cabras , Intoxicação por Plantas/diagnóstico , Intoxicação por Plantas/etiologia , Ramos Subendocárdicos/efeitos dos fármacos , Ramos Subendocárdicos/patologia , alfa-Manosidose/etiologiaRESUMO
Sida carpinifolia is a plant native to Brazil and when it is ingested by animals for a prolonged period it causes α-mannosidosis. The technique of lectin-histochemistry was applied to samples of the central nervous system (CNS) of eleven cattle naturally and experimentally intoxicated by S. carpinifolia in Brazil. The results revealed that the animals showed labeling, mainly in cerebellum sections, with the lectins Concanavalia ensiformis and Triticum vulgaris and in a lesser degree with Succinyl Triticum vulgaris. Labeling was verified within the vacuolized cytoplasm of the cerebellar Purkinje neurons, cells of the molecular layer, cells of the granular layer and astrocytes of the white matter. There was no labeling observed for the lectins Glycine max, Dolichos biflorus, Ulex europaeus-I, Arachis hypogaea and Ricinus communis-I. Intoxication by S. carpinifolia in bovine species is characterized by lectin-histochemistry as αmannosidosis, this being an important tool aiding to the diagnosis of lysosomal storage disease.(AU)
Assuntos
Animais , Bovinos , Malvaceae/química , Lisossomos/fisiologia , alfa-Manosidose/veterinária , Brasil , Sistema Nervoso Central , Técnicas Citológicas/métodosRESUMO
A neurologic disease characterized by ataxia, hypermetria, hyperesthesia, and muscle tremors of the head and neck was observed for 2 years in a flock of 28 Anglo-Nubian and Saanen goats on a farm with 5 ha of pasture. Six newborns died during the first week of life, and five abortions were recorded. The predominant plant in the pasture was Sida carpinifolia. The disease was reproduced experimentally in two goats by administration of this plant. Three goats with spontaneous disease and the two experimental animals were euthanatized and necropsied. No significant gross lesions were observed. Fragments of several organs, including the central nervous system, were processed for histopathology. Small fragments of the cerebellar cortex, liver, and pancreas of two spontaneously poisoned goats and two experimentally poisoned goats were processed for electron microscopy. Multiple cytoplasm vacuoles in hepatocytes, acinar pancreatic cells, and neurons, especially Purkinje cells, were the most striking microscopic lesions in the five animals. Ultrastructural changes included membrane-bound vacuoles in hepatocytes, Kupffer cells, acinar pancreatic cells, Purkinje cells, and the small neurons of the granular cell layer of the cerebellum. Paraffin-embedded sections of the cerebellum and pancreas were submitted for lectin histochemical analysis. The vacuoles in different cerebellar and acinar pancreatic cells reacted strongly to the following lectins: Concanavalia ensiformis, Triticum vulgaris, and succinylated Triticum vulgaris. The pattern of staining, analyzed in Purkinje cells and acinar pancreatic cells coincides with results reported for both swainsonine toxicosis and inherited mannosidosis.