A new Factor Xa inhibitor from Amblyomma cajennense with a unique domain composition

Batista, I F C; Ramos, O H P; Ventura, J S; Junqueira-de-Azevedo, I L M; Ho, P L; Chudzinski-Tavassi, A M

Batista, I F C; Ramos, O H P; Ventura, J S; Junqueira-de-Azevedo, I L M; Ho, P L; Chudzinski-Tavassi, A M.

Afiliação

Batista, I F C; Instituto Butantan. São Paulo. BR
Ramos, O H P; Instituto Butantan. São Paulo. BR
Ventura, J S; Instituto Butantan. São Paulo. BR
Junqueira-de-Azevedo, I L M; Instituto Butantan. São Paulo. BR
Ho, P L; Instituto Butantan. São Paulo. BR
Chudzinski-Tavassi, A M; Instituto Butantan. São Paulo. BR

Archives of Biochemistry and Biophysics ; 493(2): 151-156, 2010.

Article em En | SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO | ID: biblio-1059818

Biblioteca responsável: BR78.1

Localização: BR78.1

ABSTRACT

ABSTRACT

Bioactive compounds of great interest are found in the saliva of hematophagous organisms. While exploring a cDNA library derived from the salivary glands of the tick Amblyomma cajennense, a transcript that codes for a protein with unique structure (containing an N-terminal Kunitz-type domain and a C-terminus with no homology to any annotated sequences) was found. The recombinant mature form of this protein (¡13.5 kDa) was produced in Escherichia coli BL21 (DE3), and it was able to inhibit Factor Xa (FXa) and extend global blood clotting times in vitro and ex vivo. Static and dynamic predictions of its tertiary structure indicate regions that may be related to its FXa inhibitor function.

Assuntos

Animais; Anticoagulantes; Fator Xa; Saliva/fisiologia; Saliva

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Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Anticoagulantes Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Archives of Biochemistry and Biophysics Ano de publicação: 2010 Tipo de documento: Article

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