Evaluation of the in vitro Activity of Dermaseptin 01, a CationicAntimicrobial Peptide, against Schistosoma mansoni
Chemistry & Biodiversity
; 8(3): 548-558, 14 Mar , 2011.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1061935
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
Schistosomiasis is a neglected tropical disease that remains a considerable public health problem worldwide. Since the mainstay of schistosomiasis control is chemotherapy with a single drug,praziquantel, drug resistance is a concern. Here, we examined the in vitro effects of dermaseptin 01(DS 01), an antimicrobial peptide found in the skin secretion of frogs of the genus Phyllomedusa, onSchistosoma mansoni adult worms. DS 01 at a concentration of 100 mg/ml reduced the worm motor activity and caused the death of all worms within 48 h in RPMI 1640 medium. At the highest sublethal concentration of antimicrobial peptide (75 mg/ml), a 100% reduction in egg output of paired female worms was observed. Additionally, DS 01 induced morphological alterations on the tegument of S. mansoni, and a quantitative analysis carried out by confocal microscopy revealed extensive destruction of the tubercles in a dose-dependent manner over the concentration range of 50 200 mg/ml. It was the first time that an anthelmintic activity towards schistosomes has been reported for a dermaseptin.
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Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Assunto principal:
Schistosoma mansoni
/
Peptídeos Catiônicos Antimicrobianos
Idioma:
En
Revista:
Chemistry & Biodiversity
Ano de publicação:
2011
Tipo de documento:
Article