Gene expression in SK-Mel-28 human melanoma cells treated with thesnake venom jararhagin
Toxicon
; 57(1): 1-8, Jan. 2011.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1068269
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
Alternative approaches to improve the treatment of advanced melanomas are highly needed.The disintegrin domain of metalloproteinases binds integrin receptors on tumor cells,blocking migration, invasion, and metastatization. Previous studies showed that jararhagin,from the Bothrops jararaca snake venom, induces changes in the morphology and viability ofSK-Mel-28 human melanoma cells, and decreases the number of metastases in mice injected with pre-treated cells. The purpose of this study was to evaluate the molecular effects ofjararhagin on SK-Mel-28 cells and fibroblasts, concerning the expression of integrins, cadherins, caspases, and TP53 genes. Sub-toxic doses of jararhagin were administered to confluent cells. RT-PCR was performed following extraction of total RNA. Jararhagin treatmentsinduced similar morphological alterations in both normal and tumor cells, with higher IC50 values for fibroblasts. Integrin genes were downregulated in untreated cells,except for ITGA6a,b, ITGAv, and ITGB3 which were highly expressed in SK-Mel-28. The integrin expression profiles were not affected by the toxin. However, jararhagin 30 ng/mlupregulated genes TP53, CDKN1A, CDKN2A, CASP3, CASP5, CASP6, CASP8, and E-CDH in SKMel-28, and genes ITGB6, ITGB7, CASP3, TP53, and CDKN1B in fibroblasts. Appropriate jararhagin concentration can have apoptotic and suppressant effects on SK-Mel-28 cells, ratherthan on fibroblasts, and can be used to develop potential anti-cancer drugs.
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Coleções:
06-national
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BR
Base de dados:
SES-SP
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SESSP-IBACERVO
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SESSP-IBPROD
Assunto principal:
Venenos de Serpentes
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Células Cultivadas
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Bothrops
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Linhagem Celular Tumoral
Limite:
Animals
Idioma:
En
Revista:
Toxicon
Ano de publicação:
2011
Tipo de documento:
Article