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Anticancer properties of phospholipase A2 fromDaboia siamensis venom on human skin melanoma cells
Khunsap, Suchitra; Khow, Orawan; Buranapraditkun, Supranee; Suntrarachun, Sunutcha; Puthong, Songchan; Boonchang, Supatsorn.
Afiliação
  • Khunsap, Suchitra; Queen Saovabha Memorial Institute. Research and Development. Bangkok. TH
  • Khow, Orawan; Queen Saovabha Memorial Institute. Research and Development. Bangkok. TH
  • Buranapraditkun, Supranee; Chulalongkorn University. Faculty of Medicine. Department of Medicine. Clinical Immunology Unit. Cellular Immunology Laboratory Allergy. Bangkok. TH
  • Suntrarachun, Sunutcha; Queen Saovabha Memorial Institute. Research and Development. Bangkok. TH
  • Puthong, Songchan; Chulalongkorn University Institute. Institute of Biotechnology and Genetic Engineering. Bangkok. TH
  • Boonchang, Supatsorn; Queen Saovabha Memorial Institute. Research and Development. Bangkok. TH
Article em En | LILACS, VETINDEX | ID: biblio-1484683
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
Phospholipase A2 (PLA2) is a major component of theDaboia siamensis venom, which is able to hydrolyse the membrane of various cells. For this reason, the activity of PLA2was investigated regarding its pharmaceutical properties. This study was conducted to explore the pharmacological properties of a PLA2from Daboia siamensis (dssPLA2) venom on human skin melanoma cell line (SK-MEL-28). Methods dssPLA2 was isolated by ion exchange and gel filtration columns. Various concentrations of dssPLA2were investigated for cytotoxic activity and inhibition of migration on SK-MEL-28 cells. Cell death analysis, mRNA expression levels of Notch I-III and BRAF V600E genes were also determined. Results dssPLA2 exhibited cytotoxicity on SK-MEL-28 for 24 and 72 h as compared with untreated cells. However, it had no toxic effects on CCD-1064sk cells under the same conditions. dssPLA2 (0.25 and 0.5 g/mL) induced 17.16 and 30.60 % of apoptosis, while activated 6.53 and 7.05 % of necrotic cells. dssPLA2 at 0.25, 0.5, 1 and 2 g/mL could inhibit migration on SK-MEL-28 cells for 24 h by 31.06, 41.66, 50 and 68.75 %, respectively. The action of dssPLA2 significantly reduced the levels of Notch I and BRAF V600E genes expression on SK-MEL-28 cells compared with untreated cells at 72 h. Conclusions This study indicates that dssPLA2 had potential effects of apoptosis, necrosis, cytotoxicity and inhibition of migration on SK-MEL-28 cells. dssPLA2 could possibly be a selective agent that targets cancer cells without affecting normal cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Anticarcinógenos Limite: Humans Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Tailândia País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Anticarcinógenos Limite: Humans Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Tailândia País de publicação: Brasil