Avaliação das técnicas, imunodifusão em gel de agar (IDGA), ensaio imunoenzimático (ELISAi) e reação em cadeia da polimerase (PCR), para o diagnostico da brucelose ovina / Evaluation of technical, immunodiffusion agar gel (AGID), enzyme-linked immunosorbent assay (ELISAi) and polymerase chain reaction (PCR) for the diagnosis of ovine brucellosis
Rev. bras. reprod. anim
; 40(4): 330-331, Out-Dez. 2016.
Article
em Pt
| VETINDEX
| ID: biblio-1492288
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
Brucellosis in sheep has received a major focus, since it is a disease that affects the reproductive systemof animals, causing serious impairment in the productive sector. Thus, three methods for the diagnosis of ovinebrucellosis were evaluated as goal, the indirect ELISA test, the AGID technique and the PCR. Therefore, weused 211 sheep blood samples arising from properties of nine municipalities of the homogeneous micro-regionof Teresina, Piaui. The 211 blood samples were subjected to the serologic testing and PCR to detect anti-B. ovisantibodies, and Brucella ovis DNA, respectively. Positive results in serological tests were obtained, 36 (17.06%)positive in the AGID test and seven (3.31%) positive to the ELISA test, however, there were no positive results inthe PCR technique. The use of the techniques for the diagnosis of B. ovis infection is satisfactory, but the choiceof blood as the biological sample for performing the direct diagnosis of ovine brucellosis is not recommended.
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Texto completo:
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Base de dados:
VETINDEX
Assunto principal:
Brucelose
/
Ovinos
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Reação em Cadeia da Polimerase
/
Técnicas Imunoenzimáticas
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Imunodifusão
Limite:
Animals
Idioma:
Pt
Revista:
Rev. bras. reprod. anim
Ano de publicação:
2016
Tipo de documento:
Article
/
Congress and conference