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Selective inhibition of interleukin 6 receptor decreased inflammatory cytokines and increased proteases in an experimental model of critical calvarial defect
Melo, R.C.O.; Martins, A.A.; Vieira, G.H.A.; Andrade, R.V.S.; Silva, D.N.A.; Chalmers, J.; Silveira, T.M.; Pirih, F.Q.; Araújo, V.S.; Silva, J.S.P.; Lopes, M.L.D.S.; Leitão, R.F.C.; Araújo Júnior, R.F.; Silva, I.L.G.; Silva, L.J.T.; Barbosa, E.G.; Araújo, A.A..
Afiliação
  • Melo, R.C.O.; Universidade Federal do Rio Grande do Norte. Departamento de Odontologia. Natal. BR
  • Martins, A.A.; Universidade Federal do Rio Grande do Norte. Departamento de Odontologia. Natal. BR
  • Vieira, G.H.A.; Universidade Federal do Rio Grande do Norte. Departamento de Odontologia. Natal. BR
  • Andrade, R.V.S.; Universidade Federal do Rio Grande do Norte. Departamento de Odontologia. Natal. BR
  • Silva, D.N.A.; University of California. School of Dentistry. Section of Periodontics. Los Angeles. US
  • Chalmers, J.; University of California. School of Dentistry. Section of Periodontics. Los Angeles. US
  • Silveira, T.M.; University of California. School of Dentistry. Section of Periodontics. Los Angeles. US
  • Pirih, F.Q.; University of California. School of Dentistry. Section of Periodontics. Los Angeles. US
  • Araújo, V.S.; Universidade Federal do Rio Grande do Norte. Departamento de Odontologia. Natal. BR
  • Silva, J.S.P.; Universidade Federal do Rio Grande do Norte. Departamento de Odontologia. Natal. BR
  • Lopes, M.L.D.S.; Universidade Federal do Rio Grande do Norte. Departamento de Odontologia. Natal. BR
  • Leitão, R.F.C.; Universidade Federal do Ceará. Departamento de Morfologia. Fortaleza. BR
  • Araújo Júnior, R.F.; Universidade Federal do Rio Grande do Norte. Departamento de Morfologia. Natal. BR
  • Silva, I.L.G.; Universidade Federal do Rio Grande do Norte. Departamento de Morfologia. Natal. BR
  • Silva, L.J.T.; Universidade Federal do Rio Grande do Norte. Departamento de Odontologia. Natal. BR
  • Barbosa, E.G.; Universidade Federal do Rio Grande do Norte. Departamento de Ciências Farmacêuticas. Natal. BR
  • Araújo, A.A.; Universidade Federal do Rio Grande do Norte. Departamento de Biofísica e Farmacologia. Natal. BR
Braz. j. med. biol. res ; 57: e13913, fev.2024. tab, graf
Article em En | LILACS-Express | LILACS | ID: biblio-1568972
Biblioteca responsável: BR1.1
ABSTRACT
Considering the lack of consensus related to the impact of selective IL-6 receptor inhibition on bone remodeling and the scarcity of reports, especially on large bone defects, this study proposed to evaluate the biological impact of the selective inhibitor of interleukin-6 receptor (tocilizumab) in an experimental model of critical calvarial defect in rats. In this preclinical and in vivo study, 24 male Wistar rats were randomly divided into two groups (n=12/group) defect treated with collagen sponge (CG) and defect treated with collagen sponge associated with 2 mg/kg tocilizumab (TCZ). The defect in the parietal bone was created using an 8-mm diameter trephine drill. After 90 days, the animals were euthanized, and tissue samples (skull caps) were evaluated through micro-CT, histological, immunohistochemistry, cytokines, and RT-qPCR analyses. Tocilizumab reduced mononuclear inflammatory infiltration (P<0.05) and tumor necrosis factor (TNF)-α levels (P<0.01) and down-regulated tissue gene expression of BMP-2 (P<0.001), RUNX-2 (P<0.05), and interleukin (IL)-6 (P<0.05). Moreover, it promoted a stronger immunostaining of cathepsin and RANKL (P<0.05). Micro-CT and histological analyses revealed no impact on general bone formation (P>0.05). The bone cells (osteoblasts, osteoclasts, and osteocytes) in the defect area were similar in both groups (P>0.05). Tocilizumab reduced inflammatory cytokines, decreased osteogenic protein, and increased proteases in a critical bone defect in rats. Ninety days after the local application of tocilizumab in the cranial defect, we did not find a significant formation of bone tissue compared with a collagen sponge.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Braz. j. med. biol. res Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2024 Tipo de documento: Article / Project document País de afiliação: Brasil / Estados Unidos País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Braz. j. med. biol. res Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2024 Tipo de documento: Article / Project document País de afiliação: Brasil / Estados Unidos País de publicação: Brasil