Statistical Optimisation of Fermentation Media for Novel Clostridium novyi -NT Using Response Surface Methodology
Braz. arch. biol. technol
; 61: e18180026, 2018. tab, graf
Article
em En
| LILACS
| ID: biblio-974086
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT Clostridial collagenase is recognized as one of the important proteolytic enzymes used in treatment of varieties of fibro-proliferative disorders. The present work aimed to optimise the biomass concentration and collagenase enzyme activity of novel bacilli Clostridium novyi-NT. The response surface methodology tool was used to identify the optimal fermentation parameters. Central composite design (CCD) was applied with respect to three influencing factors - pH, proteose peptone and trypticase soya broth. These factors showed significant effect on the overall biomass concentration and collagenase enzyme activity (p<0.05). The maximum biomass concentration in terms of absorbance and collagenase activity (of crude enzyme) was achieved and recorded as 0.8309±0.0012 and 298.88±1.36 units/mg respectively after 22 hours of fermentation period while optimisation of media factors with help of response surface quadratic model. This is the first study to report maximum biomass concentration and collagenase activity by Clostridium novyi-NT till date by combining statistical designs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Braz. arch. biol. technol
Assunto da revista:
BIOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Índia
País de publicação:
Brasil