Characterization of clinical isolates of Streptococcus agalactiae by random amplified polymorphic DNA using degenerate oligonucleotides.
Medicina [B Aires]
; 55(6): 681-4, 1995.
Article
em En
| BINACIS
| ID: bin-37151
Biblioteca responsável:
AR2.1
ABSTRACT
Epidemiological studies of Streptococcus agalactiae strains have been limited by the lack of sensitive and discriminatory methods for comparing clinical isolates. Serotyping, albeit a widely used methodology, has been shown to possess low capability to distinguish between epidemiologically related and unrelated isolates. We have employed here a random amplification of polymorphic DNA (RAPD) assay, using degenerate oligonucleotides as primers, to characterize S. agalactiae isolates from related or unrelated clinical samples. Epidemiologically-related isolates (mother-infant pairs) showed identical profiles by this methodology. On the contrary, 12 epidemiologically-unrelated isolates (classified into 5 different serotypes) resulted in 11 distinct RAPD patterns. This suggests that the proposed modified RAPD assay provides a highly discriminatory tool for the analysis of genomic diversity among isolates from pathogenic organisms.
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Coleções:
06-national
/
AR
Base de dados:
BINACIS
Tipo de estudo:
Clinical_trials
Idioma:
En
Revista:
Medicina [B Aires]
Ano de publicação:
1995
Tipo de documento:
Article
País de publicação:
Argentina