Development of monoclonal oligobodies and chemically synthesized oligobodies.
Medicina [B Aires]
; 60 Suppl 2: 55-60, 2000.
Article
em En
| BINACIS
| ID: bin-39702
Biblioteca responsável:
AR2.1
ABSTRACT
Oligonucleotide aptamers obtained using the SELEX procedure can recognize different molecules with high affinity. However, for proteins, this recognition is limited to native conformations and the specificity has not been clearly demonstrated by methods such as Western blotting, immunohistochemistry or immunoprecipitations. Using a library of oligonucleotides and a selection strategy based on high specificity instead of high affinity, we have reported previously the preparation of polyclonal oligobodies, reagents that recognize the protein PP2A in a very specific way. Here we report a method to obtain monoclonal oligobodies. The oligobody developed specifically recognized both native and denatured states of the protein CPD1 used as a model system. We further demonstrate the specificity of the monoclonal oligobody using Western blots, immunohistochemistry, and immunoprecipitation, procedures previously limited only to antibody-based detection. In addition, a confocal microscopy is shown that was obtained using an oligobody made by chemical synthesis using an oligonucleotide synthesizer, being this the first [quot ]synthetic antibody[quot ] reported.
Buscar no Google
Coleções:
01-internacional
/
06-national
/
AR
Base de dados:
BINACIS
/
LILACS
Assunto principal:
Oligonucleotídeos
/
Anticorpos Monoclonais
/
Especificidade de Anticorpos
Limite:
Animals
Idioma:
En
/
Es
Revista:
Medicina (B.Aires)
/
Medicina [B Aires]
Assunto da revista:
MEDICINA
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Argentina