Oligobodies: bench made synthetic antibodies.
Medicina [B Aires]
; 59(6): 753-8, 1999.
Article
em En
| BINACIS
| ID: bin-40155
Biblioteca responsável:
AR2.1
ABSTRACT
Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as [quot ]synthetic antibodies[quot ]. The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named [quot ]oligobodies[quot ], has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA sequence from a database provides enough information to make them.
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Coleções:
06-national
/
AR
Base de dados:
BINACIS
Idioma:
En
Revista:
Medicina [B Aires]
Ano de publicação:
1999
Tipo de documento:
Article
País de publicação:
Argentina