A alpha-glycerophosphate dehydrogenase is present in Trypanosoma cruzi glycosomes
Mem. Inst. Oswaldo Cruz
; 96(5): 697-701, July 2001. graf, tab
Article
em En
| LILACS
| ID: lil-289360
Biblioteca responsável:
BR1.1
ABSTRACT
alpha-glycerophosphate dehydrogenase (alpha-GPDH-EC.1.1.1.8) has been considered absent in Trypanosoma cruzi in contradiction with all other studied trypanosomatids. After observing that the sole malate dehydrogenase can not maintain the intraglycosomal redox balance, GPDH activity was looked for and found, although in very variable levels, in epimastigotes extracts. GPDH was shown to be exclusively located in the glycosome of T. cruzi by digitonin treatment and isopycnic centrifugation. Antibody against T. brucei GPDH showed that this enzyme seemed to be present in an essentially inactive form at the beginning of the epimastigotes growth. GPDH is apparently linked to a salicylhydroxmic-sensitive glycerophosphate reoxidizing system and plays an essential role in the glycosome redox balance
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Trypanosoma cruzi
/
Glicerolfosfato Desidrogenase
/
Microcorpos
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2001
Tipo de documento:
Article
/
Project document
País de afiliação:
Venezuela
País de publicação:
Brasil