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A glycoprotein with anti-inflammatory properties secreted by an Aspergillus nidulans modified strain
Queiroz, J. C. F; Teixeira, C. F. P; Lebrun, I; Spencer, P. J; Zucchi, T. M. A. D.
Afiliação
  • Queiroz, J. C. F; University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology and Biotechnology Research Center. São Paulo. BR
  • Teixeira, C. F. P; Butantan Institute. Laboratory of Pharmacology. São Paulo. BR
  • Lebrun, I; Butantan Institute. Biochemistry and Biophysics Laboratorty. São Paulo. BR
  • Spencer, P. J; Nuclear Energy Research Institute. Biotechnology Center. São Paulo. BR
  • Zucchi, T. M. A. D; University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology and Biotechnology Research Center. São Paulo. BR
J. venom. anim. toxins incl. trop. dis ; 13(2): 527-543, 2007. graf, tab
Article em En | LILACS, SES-SP | ID: lil-452845
Biblioteca responsável: BR33.1
ABSTRACT
Total RNA from lipopolysaccharide (LPS)-stimulated rat macrophages used to treat protoplasts from an Aspergillus nidulans strain originated the RT2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in Wistar rats. The protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. The screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male Swiss mice. Biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total sugar quantification revealed 32 percent glycosylation of the protein fraction. Amino acid analysis of such fraction showed a peculiar pattern presenting 29 percent valine. SDS-PAGE revealed that the protein sample is pure and its molecular weight is about 40kDa. Intravenous injection of the isolated substance into mice significantly inhibited carrageenan-induced paw edema. The isolated glycoprotein decreased carrageenan-induced paw edema in a prostaglandin-dependent phase, suggesting an inhibitory effect of the isolated glycoprotein on prostaglandin synthesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / SES-SP Assunto principal: Aspergilose / Aspergillus nidulans / Glicoproteínas / Anti-Inflamatórios Limite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / SES-SP Assunto principal: Aspergilose / Aspergillus nidulans / Glicoproteínas / Anti-Inflamatórios Limite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil