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Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis
Silva, D. F; Assis, C. M; Zamboni, I. M; Barreto, L. C; Kohara, V. S; Vicentini-Moreira, A. P.
Afiliação
  • Silva, D. F; Adolfo Lutz Institute. Immunology Section. Laboratory of Immunodiagnosis in Mycosis. São Paulo. BR
  • Assis, C. M; Adolfo Lutz Institute. Basic Service Division. São Paulo. BR
  • Zamboni, I. M; Adolfo Lutz Institute. Immunology Section. Laboratory of Immunodiagnosis in Mycosis. São Paulo. BR
  • Barreto, L. C; Adolfo Lutz Institute. Immunology Section. Laboratory of Immunodiagnosis in Mycosis. São Paulo. BR
  • Kohara, V. S; Adolfo Lutz Institute. Immunology Section. Laboratory of Immunodiagnosis in Mycosis. São Paulo. BR
  • Vicentini-Moreira, A. P; Adolfo Lutz Institute. Immunology Section. Laboratory of Immunodiagnosis in Mycosis. São Paulo. BR
J. venom. anim. toxins incl. trop. dis ; 14(2): 313-321, 2008. ilus, tab
Article em En | LILACS | ID: lil-484567
Biblioteca responsável: BR33.1
ABSTRACT
The purpose of this work was to evaluate two serological assays double immunodiffusion (DI) and immunoblotting (IB) in immunodiagnosis of paracoccidioidomycosis (PCM). We evaluated by IB assay 23 sera samples from patients with clinical confirmation of PCM, all of them with negative DI results against culture filtrate from Paracoccidioides brasiliensis isolate 113. For IB, as well as for comparative DI assay, we employed soluble components of the cell wall outer surface (SCCWOS) from P. brasiliensis isolate 113 cultivated at 36°C in Fava-Neto's agar medium for 5 and 10 days. Among the 20 sera samples analyzed by DI, 13 (65 percent) were negative and 7 (35 percent) were positive against SCCWOS obtained on the 5th and 10th days. By IB assay, 95.4 percent and 100 percent of sera reacted against gp43 and gp70 present in SCCWOS from the 5th day and 95.6 percent recognized these fractions when evaluated against SCCWOS from the 10th day. Our results demonstrated that the use of an immunoenzymatic assay significantly improves the sensitivity of PCM immunodiagnosis and also suggests that at least two serological tests for antibody detection should be adopted in cases of questionable diagnosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Paracoccidioidomicose / Immunoblotting Tipo de estudo: Diagnostic_studies Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Paracoccidioidomicose / Immunoblotting Tipo de estudo: Diagnostic_studies Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil