Your browser doesn't support javascript.
loading
Influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, Paracoccus pantotrophus P16 a strain from shrimp pond
Pintathong, Punyawatt; Richardson, David J; Spiro, Stephen; Choorit, Wanna.
Afiliação
  • Pintathong, Punyawatt; Walailak University. School of Agricultural Technology. Biotechnology Program. Thasala. TH
  • Richardson, David J; University of East Anglia. School of Biological Sciences. Faculty of Science. Norwich. GB
  • Spiro, Stephen; University of Texas at Dallas. Department of Molecular and Cell Biology. Richardson. US
  • Choorit, Wanna; Walailak University. School of Agricultural Technology. Biotechnology Program. Thasala. TH
Electron. j. biotechnol ; 12(2): 4-5, Apr. 2009. ilus, tab
Article em En | LILACS | ID: lil-551365
Biblioteca responsável: CL1.1
ABSTRACT
The effect of metal ions, ferric ion (Fe3+) and molybdenum ion (Mo6+) on the denitrification process of Paracoccus pantotrophus P16 grown under saline conditions was investigated. Results revealed that the dosages of added Fe3+ and Mo6+ significantly accelerated nitrate utilization and nitrite accumulation. Enzymatic studies revealed that the membrane-bound nitrate reductase and the periplasmic nitrite reductase had activities of 998 +/- 28 and 373 +/- 18 nmol (mg protein)-1 min-1, respectively after growing Paracoccus pantotrophus P16 in medium supplemented with 1.5 micron M Fe3+. If provided with 1.5 micron M Fe3+and 2.4 micron M Mo6+, the membrane-bound nitrate reductase activity increased to 6,223 +/- 502 nmol (mg protein)-1 min-1 and the periplasmic nitrite reductase was 344 +/- 20 nmol (mg protein)-1 min-1. The results indicated that an addition of Fe3+ and Mo6+ led to an overstimulation of nitrate reductase activity as compared with nitrite reductase activity. When glucose was supplied, the minimal ratio of carbon per nitrate (C/N) was 2.31 mg C/mg NO3--N with denitrification yield of 0.45 g NO3--N/g C. Addition of ethanol instead of glucose, the minimal ratio of C/N was 1.15 mg C/mg NO3--N with denitrification yield of 1.08 g NO3--N/g C.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Artemia / Paracoccus pantotrophus / Molibdoferredoxina Idioma: En Revista: Electron. j. biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2009 Tipo de documento: Article / Project document País de afiliação: Tailândia / Estados Unidos / Reino Unido País de publicação: Chile
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Artemia / Paracoccus pantotrophus / Molibdoferredoxina Idioma: En Revista: Electron. j. biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2009 Tipo de documento: Article / Project document País de afiliação: Tailândia / Estados Unidos / Reino Unido País de publicação: Chile