CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
Braz. j. med. biol. res
; 44(3): 200-205, Mar. 2011. ilus, tab
Article
em En
| LILACS
| ID: lil-576062
Biblioteca responsável:
BR1.1
ABSTRACT
Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Agregação Celular
/
Proliferação de Células
/
Células-Tronco de Carcinoma Embrionário
/
Fator de Crescimento do Tecido Conjuntivo
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Braz. j. med. biol. res
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil