Your browser doesn't support javascript.
loading
Analysing deltamethrin susceptibility and pyrethroid esterase activity variations in sylvatic and domestic Triatoma infestans at the embryonic stage
Santo-Orihuela, Pablo Luis; Carvajal, Guillermo; Picollo, Maria Ines; Vassena, Claudia Viviana.
Afiliação
  • Santo-Orihuela, Pablo Luis; Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Cientificas y Tecnicas para la Defensa. Centro de Investigaciones en Plagas e Insecticidas,. Buenos Aires. AR
  • Carvajal, Guillermo; Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Cientificas y Tecnicas para la Defensa. Centro de Investigaciones en Plagas e Insecticidas,. Buenos Aires. AR
  • Picollo, Maria Ines; Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Cientificas y Tecnicas para la Defensa. Centro de Investigaciones en Plagas e Insecticidas,. Buenos Aires. AR
  • Vassena, Claudia Viviana; Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Cientificas y Tecnicas para la Defensa. Centro de Investigaciones en Plagas e Insecticidas,. Buenos Aires. AR
Mem. Inst. Oswaldo Cruz ; 108(8): 1031-1036, 6/dez. 2013. tab, graf
Article em En | LILACS | ID: lil-697150
Biblioteca responsável: BR1.1
ABSTRACT
The aim of the present work was to study the deltamethrin susceptibility of eggs from Triatoma infestans populations and the contribution of pyrethroid esterases to deltamethrin degradation. Insects were collected from sylvatic areas, including Veinte de Octubre and Kirus-Mayu (Bolivia) and from domiciliary areas, including El Palmar (Bolivia) and La Pista (Argentina). Deltamethrin susceptibility was determined by dose-response bioassays. Serial dilutions of deltamethrin (0.0005-1 mg/mL) were topically applied to 12-day-old eggs. Samples from El Palmar had the highest lethal dose ratio (LDR) value (44.90) compared to the susceptible reference strain (NFS), whereas the Veinte de Octubre samples had the lowest value (0.50). Pyrethroid esterases were evaluated using 7-coumaryl permethrate (7-CP) on individually homogenised eggs from each population and from NFS. The El Palmar and La Pista samples contained 40.11 and 36.64 pmol/min/mg protein, respectively, and these values were statistically similar to NFS (34.92 pmol/min/mg protein) and different from Kirus-Mayu and Veinte de Octubre (27.49 and 22.69 pmol/min/mg protein, respectively). The toxicological data indicate that the domestic populations were resistant to deltamethrin, but no statistical contribution of 7-CP esterases was observed. The sylvatic populations had similar LDR values to NFS, but lower 7-CP esterase activities. Moreover, this is the first study of the pyrethroid esterases on T. infestans eggs employing a specific substrate (7-CP).
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Óvulo / Piretrinas / Triatoma / Esterases / Nitrilas Limite: Animals Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Argentina País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Óvulo / Piretrinas / Triatoma / Esterases / Nitrilas Limite: Animals Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Argentina País de publicação: Brasil