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Chemotaxonomic Fingerprinting of Chilean Lichens Through Maldi and Electrospray Ionization Mass Spectrometry
Santos, Leonardo Silva; Soriano, Maria del Pilar Camarantin; Mirabal-Gallardo, Yaneris; Carrasco-Sanchez, Veronica; Nachtigall, Fabiane Manke; Pereira, Iris; Pereira, Eugenia.
Afiliação
  • Santos, Leonardo Silva; Talca University. Chemistry Institute of Natural Resources. Talca. CL
  • Soriano, Maria del Pilar Camarantin; Talca University. Chemistry Institute of Natural Resources. Talca. CL
  • Mirabal-Gallardo, Yaneris; Talca University. Chemistry Institute of Natural Resources. Talca. CL
  • Carrasco-Sanchez, Veronica; Talca University. Chemistry Institute of Natural Resources. Talca. CL
  • Nachtigall, Fabiane Manke; Talca University. Chemistry Institute of Natural Resources. Talca. CL
  • Pereira, Iris; Talca University. Chemistry Institute of Natural Resources. Talca. CL
  • Pereira, Eugenia; Talca University. Chemistry Institute of Natural Resources. Talca. CL
Braz. arch. biol. technol ; 58(2): 244-253, Mar-Apr/2015. tab, graf
Article em En | LILACS | ID: lil-744308
Biblioteca responsável: BR1.1
ABSTRACT
The aim of this work was to study a fast, new, sensitive, and simple method for the chemotaxonomic classification of Chilean lichens (Teloschistes chrysophthalmus, Ramalina farinacea, Usnea pusilla, Ramalina chilensis and Stereocaulon ramulosum) using MALDI-TOF-MS and UPLC-ESI(-)-MS data. Lichens soluble proteins fingerprints were acquired by MALDI-TOF-MS and they were analyzed by chemometric (PCA). Lichens organic extracts fingerprints were obtained by UPLC-ESI(-)-MS. MALDI-TOF-MS associated with chemometric analysis was used to detect new m/z patterns of soluble proteins that were compared with Protein Data Bank of UnitPro. These data also permitted the satisfactory distinction among the families and species. UPLC-ESI(-)-MS fingerprints analyses of the organic extracts showed the presence of five major lichen compounds (atranorin, parietin, teloschistin, ramalinolic and usnic acids). In contrast to other techniques, MALDI-TOF-MS associated with chemometric analysis and UPLC-ESI(-)-MS provided a new, fast and sensitive method for chemotaxonomic characterization of lichens.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS País/Região como assunto: America do sul / Chile Idioma: En Revista: Braz. arch. biol. technol Assunto da revista: BIOLOGIA Ano de publicação: 2015 Tipo de documento: Article / Project document País de afiliação: Chile País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS País/Região como assunto: America do sul / Chile Idioma: En Revista: Braz. arch. biol. technol Assunto da revista: BIOLOGIA Ano de publicação: 2015 Tipo de documento: Article / Project document País de afiliação: Chile País de publicação: Brasil