Your browser doesn't support javascript.
loading
Molecular characterisation of Aspergillus flavus isolates from peanut fields in India using AFLP
Singh, Diwakar; Radhakrishnan, T.; Kumar, Vinod; Bagwan, N.B.; Basu, M.S.; Dobaria, J.R.; Mishra, Gyan P.; Chanda, S.V..
Afiliação
  • Singh, Diwakar; Directorate of Groundnut Research. Gujarat. IN
  • Radhakrishnan, T.; Directorate of Groundnut Research. Gujarat. IN
  • Kumar, Vinod; Directorate of Groundnut Research. Gujarat. IN
  • Bagwan, N.B.; Directorate of Groundnut Research. Gujarat. IN
  • Basu, M.S.; Directorate of Groundnut Research. Gujarat. IN
  • Dobaria, J.R.; Directorate of Groundnut Research. Gujarat. IN
  • Mishra, Gyan P.; Directorate of Groundnut Research. Gujarat. IN
  • Chanda, S.V.; Directorate of Groundnut Research. Gujarat. IN
Braz. j. microbiol ; 46(3): 673-682, July-Sept. 2015. tab, ilus
Article em En | LILACS | ID: lil-755832
Biblioteca responsável: BR1.1
ABSTRACT

Aflatoxin contamination of peanut, due to infection by Aspergillus flavus, is a major problem of rain-fed agriculture in India. In the present study, molecular characterisation of 187 Aspergillus flavus isolates, which were sampled from the peanut fields of Gujarat state in India, was performed using AFLP markers. On a pooled cluster analysis, the markers could successfully discriminate among the ‘A’, ‘B’ and ‘G’ group A. flavus isolates. PCoA analysis also showed equivalent results to the cluster analysis. Most of the isolates from one district could be clustered together, which indicated genetic similarity among the isolates. Further, a lot of genetic variability was observed within a district and within a group. The results of AMOVA test revealed that the variance within a population (84%) was more than that between two populations (16%). The isolates, when tested by indirect competitive ELISA, showed about 68.5% of them to be atoxigenic. Composite analysis between the aflatoxin production and AFLP data was found to be ineffective in separating the isolate types by aflatoxigenicity. Certain unique fragments, with respect to individual isolates, were also identified that may be used for development of SCAR marker to aid in rapid and precise identification of isolates.

.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Arachis / Aspergillus flavus / Aflatoxinas País/Região como assunto: Asia Idioma: En Revista: Braz. j. microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Índia País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Arachis / Aspergillus flavus / Aflatoxinas País/Região como assunto: Asia Idioma: En Revista: Braz. j. microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Índia País de publicação: Brasil