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Role of DNA ploidy in diagnosis and prognosis of high-grade cervical intraepithelial neoplasia: A prospective cohort study.
Pogere, Adriane; Costa, Ane Francyne; Pasinato, Ana Paula Beltrame Farina; Machado, Marcos José; de Miranda Onofre, Fabiana Botelho; Onofre, Alexandre Sherlley Casimiro.
Afiliação
  • Pogere A; Department of Obstetrics and Gynecology, Professor Polydoro Ernani of Sao Thiago University Hospital of the Federal University of Santa Catarina, Florianópolis, Florianópolis, Brazil.
  • Costa AF; Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Pasinato APBF; Department of Pathology, Professor Polydoro Ernani of Sao Thiago University Hospital of the Federal University of Santa Catarina, Florianópolis, Florianópolis, Brazil.
  • Machado MJ; Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, Brazil.
  • de Miranda Onofre FB; Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Onofre ASC; Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, Brazil.
Cytopathology ; 35(1): 122-130, 2024 Jan.
Article em En | MEDLINE | ID: mdl-37872834
ABSTRACT

OBJECTIVE:

To compare the sensitivity and specificity of DNA ploidy with cytology, human papillomavirus (HPV) testing and colposcopy in diagnosis of high-grade cervical intraepithelial neoplasia (CIN) and to assess the role of aneuploidy in cervical lesions with the worst prognosis. A prospective observational cohort study was conducted on 254 women with altered colpocytology.

METHODS:

Colposcopy, biopsy, DNA-ICM and HPV examinations were applied to cervical cytological and histological samples. Participants were evaluated every 6 months and divided into two groups 'Harm' and 'No-harm'. Logistic regression and multivariate COX model were used to identify independent risk factors for diagnosis and prognosis of high-grade CIN, and ROC curve to assess the sensitivity and specificity of methods.

RESULTS:

Variables 'age greater than or equal to 30 years', 'lesion size greater than 20%', 'aneuploidy' and 'HPV 16' were associated with diagnosis of high-grade CIN and 'aneuploidy' and 'women living with HIV', with a worse prognosis. Agreement for colposcopy was good, with a sensitivity of 79.3% and specificity of 94.4%; DNA-ICM and cytology were moderate, with sensitivity of 74.6% and 72.3% and specificity of 85.3% and 76.1%, respectively. High-risk HPV and HPV 16 tests were weak, with sensitivity of 75.0% and 43.75% and specificity of 50.0% and 88.64%, respectively.

CONCLUSIONS:

In relation to high-grade CIN diagnosis, DNA-ICM presented similar sensitivity and specificity to cytology and high-risk HPV test when associated with HPV 16. Regarding prognosis, this research certifies that aneuploidy is considered a predictor of more severe cervical injury.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Displasia do Colo do Útero / Neoplasias do Colo do Útero / Infecções por Papillomavirus Limite: Female / Humans / Pregnancy Idioma: En Revista: Cytopathology Assunto da revista: PATOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Displasia do Colo do Útero / Neoplasias do Colo do Útero / Infecções por Papillomavirus Limite: Female / Humans / Pregnancy Idioma: En Revista: Cytopathology Assunto da revista: PATOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido