Your browser doesn't support javascript.
loading
Bothrops snake venom L-amino acid oxidases impair biofilm formation of clinically relevant bacteria.
Alves de Melo Fernandes, Thales; Rafaella Costa, Tássia; de Paula Menezes, Ralciane; Arantes de Souza, Meliza; Gomes Martins, Carlos Henrique; Junior, Nilson Nicolau; Gobbi Amorim, Fernanda; Quinton, Loïc; Polloni, Lorena; Teixeira, Samuel Cota; Amália Vieira Ferro, Eloisa; Soares, Andreimar Martins; de Melo Rodrigues Ávila, Veridiana.
Afiliação
  • Alves de Melo Fernandes T; Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • Rafaella Costa T; Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • de Paula Menezes R; Laboratory of Antimicrobial Testing, Institute of Biomedical Science, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • Arantes de Souza M; Laboratory of Antimicrobial Testing, Institute of Biomedical Science, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • Gomes Martins CH; Laboratory of Antimicrobial Testing, Institute of Biomedical Science, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • Junior NN; Laboratory of Molecular Modeling, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • Gobbi Amorim F; Mass Spectrometry Laboratory, MolSys RU, University of Liège, 4000 Liège, Belgium.
  • Quinton L; Mass Spectrometry Laboratory, MolSys RU, University of Liège, 4000 Liège, Belgium.
  • Polloni L; Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • Teixeira SC; Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Federal University of Uberlândia, MG, Brazil.
  • Amália Vieira Ferro E; Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Federal University of Uberlândia, MG, Brazil.
  • Soares AM; Laboratory of Biotechnology of Proteins and Bioactive Compounds in the Western Amazon (LABIOPROT), Oswaldo Cruz Foundation, FIOCRUZ Rondônia, Federal University of Rondônia (UNIR), And National Institute of Science and Technology of Epidemiology of the Western Amazon, INCT-EPIAMO, Porto Velho-RO, Br
  • de Melo Rodrigues Ávila V; Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil. Electronic address: veridiana@ufu.br.
Toxicon ; 238: 107569, 2024 Feb 01.
Article em En | MEDLINE | ID: mdl-38122835
ABSTRACT
The present work addressed the abilities of two L-amino acid oxidases isolated from Bothrops moojeni (BmooLAAO-I) and Bothrops jararacussu (BjussuLAAO-II) snake venoms to control the growth and prevent the biofilm formation of clinically relevant bacterial pathogens. Upon S. aureus (ATCC BAA44) and S. aureus (clinical isolates), BmooLAAO-I (MIC = 0.12 and 0.24 µg/mL, respectively) and BjussuLAAO-II (MIC = 0.15 µg/mL) showed a potent bacteriostatic effect. Against E. coli (ATCC BAA198) and E. coli (clinical isolates), BmooLAAO-I (MIC = 15.6 and 62.5 µg/mL, respectively) and BjussuLAAO-II (MIC = 4.88 and 9.76 µg/mL, respectively) presented a lower extent effect. Also, BmooLAAO-I (MICB50 = 0.195 µg/mL) and BjussuLAAO-II (MICB50 = 0.39 µg/mL) inhibited the biofilm formation of S. aureus (clinical isolates) in 88% and 89%, respectively, and in 89% and 53% of E. coli (clinical isolates). Moreover, scanning electron microscopy confirmed that the toxins affected bacterial morphology by increasing the roughness of the cell surface and inhibited the biofilm formation. Furthermore, analysis of the tridimensional structures of the toxins showed that the surface-charge distribution presents a remarkable positive region close to the glycosylation motif, which is more pronounced in BmooLAAO-I than BjussuLAAO-II. This region may assist the interaction with bacterial and biofilm surfaces. Collectively, our findings propose that venom-derived antibiofilm agents are promising biotechnological tools which could provide novel strategies for biofilm-associated infections.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bothrops / Venenos de Crotalídeos / Serpentes Peçonhentas Limite: Animals Idioma: En Revista: Toxicon Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bothrops / Venenos de Crotalídeos / Serpentes Peçonhentas Limite: Animals Idioma: En Revista: Toxicon Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido