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Comet assay as a suitable biomarker for in vivo oral carcinogenesis: a systematic review.
Guedes Pinto, Thiago; de Souza, Daniel Vitor; da Silva, Glenda Nicioli; Salvadori, Daisy Maria Favero; Martins, Manoela Domingues; Ribeiro, Daniel Araki.
Afiliação
  • Guedes Pinto T; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Santos, SP, Brazil.
  • de Souza DV; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Santos, SP, Brazil.
  • da Silva GN; Department of Clinical Analysis, School of Pharmacy, Federal University of Ouro Preto, UFOP, Ouro Preto, MG, Brazil.
  • Salvadori DMF; Department of Pathology, Botucatu Medical School, Sao Paulo State University, UNESP, Botucatu, SP, Brazil.
  • Martins MD; Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, UFRS, Porto Alegre, RS, Brazil.
  • Ribeiro DA; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Santos, SP, Brazil.
Biomarkers ; 28(8): 692-702, 2023 Dec.
Article em En | MEDLINE | ID: mdl-38131287
ABSTRACT
BACKGROUND AND

OBJECTIVES:

In order to detect genetic damage, different methods have been developed, such as micronuclei and comet assay. The comet assay presents some advantages when compared to the other aforementioned methods, including wide versatility, as any eukaryotic cell can be evaluated at an individual cellular level. In this context, the aim of this systematic review was designed to help further elucidate the following question is the comet assay a suitable biomarker of in vivo oral carcinogenesis? MATERIAL AND

METHODS:

The present systematic review was performed in accordance with the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines. Full manuscripts from 18 studies were carefully selected in this setting.

RESULTS:

A total of 15 studies demonstrated positive findings for genotoxicity in peripheral blood or oral cells in patients with pre-malignant lesions or oral cancer. In the quality assessment of studies, 1 was classified as Strong, 5 were considered as Moderate, and 12 were classified as Weak.

CONCLUSION:

In summary, the comet assay can be a useful biomarker for oral carcinogenesis. However, further studies with more strict parameters are suggested (with less uncontrolled confounders) in order to increase findings reliability for diagnosis of oral potentially malignant lesions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dano ao DNA / Neoplasias Bucais Tipo de estudo: Systematic_reviews Limite: Humans Idioma: En Revista: Biomarkers Assunto da revista: BIOQUIMICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dano ao DNA / Neoplasias Bucais Tipo de estudo: Systematic_reviews Limite: Humans Idioma: En Revista: Biomarkers Assunto da revista: BIOQUIMICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido