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HPLC method for quantifying verbascoside in Stizophyllum perforatum and assessment of verbascoside acute toxicity and antileishmanial activity.
Alves, Osvaine Junior Alvarenga; Ozelin, Saulo Duarte; Magalhães, Larissa Fernandes; Candido, Ana Carolina Bolela Bovo; Gimenez, Valéria Maria Melleiro; Silva, Márcio Luís Andrade E; Cunha, Wilson Roberto; Januário, Ana Helena; Tavares, Denise Crispim; Magalhães, Lizandra Guidi; Pauletti, Patricia Mendonça.
Afiliação
  • Alves OJA; Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil.
  • Ozelin SD; Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil.
  • Magalhães LF; Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil.
  • Candido ACBB; Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil.
  • Gimenez VMM; Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil.
  • Silva MLAE; Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil.
  • Cunha WR; Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil.
  • Januário AH; Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil.
  • Tavares DC; Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil.
  • Magalhães LG; Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil.
  • Pauletti PM; Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil.
Front Plant Sci ; 14: 1324680, 2023.
Article em En | MEDLINE | ID: mdl-38143582
ABSTRACT
We report the chemical composition of the crude leaf extracts obtained from Stizophyllum perforatum (Cham.) Miers (Bignoniaceae), a simple high-performance liquid chromatography-diode array detection (HPLC-DAD) method based on mangiferin as an internal standard to quantify verbascoside, and the verbascoside acute oral toxicity and antileishmanial activity. HPLC-high-resolution mass spectrometry-DAD (HPLC-HRMS-DAD) analyses of the crude ethanol S. perforatum leaf extracts (CE-1 and CE-2) revealed that verbascoside was the major constituent in both extracts. CE-1 was purified, and verbascoside and casticin, among other compounds, were isolated. The developed HPLC-DAD method was validated and met the required standards. Investigation of the CE-2 acute toxicity indicated a lethal dose (LD50) greater than 2,000 mg/kg of body weight. Both CE-1 and CE-2 exhibited antileishmanial activity. The isolated compounds, verbascoside and casticin, also displayed antileishmanial activity with effective concentrations (IC50) of 6.23 and 24.20 µM against promastigote forms and 3.71 and 18.97 µM against amastigote forms of Leishmania amazonensis, respectively, but they were not cytotoxic to J774A.1 macrophages. Scanning electron microscopy of the L. amazonensis promastigotes showed that the parasites became more rounded and that their plasma membrane was altered in the presence of verbascoside. Additionally, transmission electron microscopy demonstrated that vacuoles emerged, lipids accumulated, kinetoplast size increased, and interstitial extravasation occurred in L. amazonensis promastigotes exposed to verbascoside. These findings suggest that S. perforatum is a promising candidate for further in vivo investigations against L. amazonensis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Plant Sci Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Plant Sci Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça