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Photobiomodulation effects on fibroblasts and keratinocytes after ionizing radiation and bacterial stimulus.
Monteiro, Mylene Martins; Amorim Dos Santos, Juliana; Paiva Barbosa, Victor; Rezende, Taia Maria Berto; Guerra, Eliete Neves Silva.
Afiliação
  • Monteiro MM; University of Brasília, Brasília, Laboratory of Oral Histopathology, Health Sciences Faculty, Brazil.
  • Amorim Dos Santos J; University of Brasília, Brasília, Laboratory of Oral Histopathology, Health Sciences Faculty, Brazil.
  • Paiva Barbosa V; University of Brasília, Brasília, Laboratory of Oral Histopathology, Health Sciences Faculty, Brazil.
  • Rezende TMB; University of Brasília, Brasília, Laboratory of Oral Histopathology, Health Sciences Faculty, Brazil.
  • Guerra ENS; University of Brasília, Brasília, Laboratory of Oral Histopathology, Health Sciences Faculty, Brazil. Electronic address: elieteneves@unb.br.
Arch Oral Biol ; 159: 105874, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38147800
ABSTRACT

OBJECTIVE:

Photobiomodulation therapy (PBMT) has proven to reduce inflammation and pain and increase wound healing. Thus, the aim of this study was to analyze the effects of PBMT parameters on migration, proliferation, and gene expression after ionizing radiation and bacterial-induced stress in an in vitro study.

DESIGN:

Keratinocytes (HaCaT) and Fibroblasts (HGFs) were grown in DMEM with 10 % fetal bovine serum until stressful condition induction with lipopolysaccharide (LPS) of Escherichia coli (1 µg/mL), Porphyromonas gingivalis protein extract (5 µg/mL) and ionizing radiation (8 Gy). Low-laser irradiation (660 nm, 30 mW) was carried out in four sessions, with 6 h intervals, and energy density of 2, 3, 4, and 5 J/cm². Scratch assays, immunofluorescence, and RT-qPCR were performed.

RESULTS:

Treated fibroblasts and keratinocytes showed significant response in proliferation and migration after scratch assays (p < 0.05). Higher expressions of α-SMA in fibroblasts and F-actin in keratinocytes were observed in cells subjected to 3 J/cm². PI3K-pathway genes expression tended to enhance in fibroblasts, presenting a higher relative expression when compared to keratinocytes. In keratinocytes, PBMT groups demonstrated deregulated expression for all inflammatory cytokines' genes tested while fibroblasts presented a tendency to enhance those genes expression in a dose dependent way.

CONCLUSIONS:

The present study showed that delivering 660 nm, 30 mW was effective to stimulate cell migration, proliferation and to accelerate wound healing. PBMT can modulate cytokines and pathways involved in wound repair. The different energy densities delivering distinct responses in vitro highlights that understanding laser parameters is fundamental to improve treatment strategies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Terapia com Luz de Baixa Intensidade Idioma: En Revista: Arch Oral Biol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Terapia com Luz de Baixa Intensidade Idioma: En Revista: Arch Oral Biol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido