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IL-1ß Antagonist Receptor Peptide Associated with Photobiomodulation Accelerates Diabetic Wound Tissue Repair.
Zaccaron, Rubya Pereira; de Roch Casagrande, Laura; Venturini, Ligia Milanez; Bittencourt, João Vitor Silvano; da Costa, Camila; de Pieri, Ellen; Thirupathi, Anand; Rezin, Gislaine Tezza; Machado-de-Ávila, Ricardo Andrez; Silveira, Paulo Cesar Lock.
Afiliação
  • Zaccaron RP; Laboratory of Experimental Physiopathology, Program of Postgraduate in Science of Health, Universidade Do Extremo Sul Catarinense, Santa Catarina State, Av. Universitária, 1105 Universitário-Block S, Room 17, Criciúma, 88806-000, Brazil.
  • de Roch Casagrande L; Laboratory of Experimental Physiopathology, Program of Postgraduate in Science of Health, Universidade Do Extremo Sul Catarinense, Santa Catarina State, Av. Universitária, 1105 Universitário-Block S, Room 17, Criciúma, 88806-000, Brazil.
  • Venturini LM; Laboratory of Experimental Physiopathology, Program of Postgraduate in Science of Health, Universidade Do Extremo Sul Catarinense, Santa Catarina State, Av. Universitária, 1105 Universitário-Block S, Room 17, Criciúma, 88806-000, Brazil.
  • Bittencourt JVS; Laboratory of Experimental Physiopathology, Program of Postgraduate in Science of Health, Universidade Do Extremo Sul Catarinense, Santa Catarina State, Av. Universitária, 1105 Universitário-Block S, Room 17, Criciúma, 88806-000, Brazil.
  • da Costa C; Laboratory of Experimental Physiopathology, Program of Postgraduate in Science of Health, Universidade Do Extremo Sul Catarinense, Santa Catarina State, Av. Universitária, 1105 Universitário-Block S, Room 17, Criciúma, 88806-000, Brazil.
  • de Pieri E; Laboratory of Experimental Physiopathology, Program of Postgraduate in Science of Health, Universidade Do Extremo Sul Catarinense, Santa Catarina State, Av. Universitária, 1105 Universitário-Block S, Room 17, Criciúma, 88806-000, Brazil.
  • Thirupathi A; Research Academy of Medicine Combining Sports, Ningbo No. 2 Hospital, Ningbo, 315099, China.
  • Rezin GT; Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNISUL), Tubarão, Santa Catarina, Brazil.
  • Machado-de-Ávila RA; Laboratory of Experimental Physiopathology, Program of Postgraduate in Science of Health, Universidade Do Extremo Sul Catarinense, Santa Catarina State, Av. Universitária, 1105 Universitário-Block S, Room 17, Criciúma, 88806-000, Brazil.
  • Silveira PCL; Laboratory of Experimental Physiopathology, Program of Postgraduate in Science of Health, Universidade Do Extremo Sul Catarinense, Santa Catarina State, Av. Universitária, 1105 Universitário-Block S, Room 17, Criciúma, 88806-000, Brazil. psilveira@unesc.net.
Inflammation ; 47(4): 1262-1277, 2024 Aug.
Article em En | MEDLINE | ID: mdl-38236386
ABSTRACT
Chronic hyperglycemia caused by diabetes mellitus (DM) slows down the healing process due to prolonged inflammation which impedes the regeneration progression. Photobiomodulation (PBM) is considered a non-pharmacological intervention and has anti-inflammatory and biostimulatory effects that accelerate the healing process. Currently found IL-1ß inhibitors are difficult to implement due to their cytotoxic potential, excessive amounts, and invasive administration, and therefore, the application of this peptide in diabetic wounds represents a promising intervention to help resolve the inflammatory response. This study aimed to investigate the effect of an IL-1ß inhibitor molecule associated with PBM irradiation in a model of epithelial injury in diabetic mice. After the induction of the DM model with streptozotocin (STZ), the skin lesion model was implemented through surgical excision. Sixty C57BL/6 mice divided into five experimental groups (n = 12) were used excisional wound (EW), DM + EW, DM + EW + DAP 1-2 (inhibitor peptide), DM + EW + PBM, and DM + EW + PBM + DAP 1-2. Treatment started 12 h after wound induction and was performed daily for 5 days. Twenty-four hours after the last application, the animals were euthanized and the outer edge of the wound was removed. The results obtained demonstrate that the DM + EW + PBM + DAP 1-2 group caused a reduction in the levels of pro-inflammatory cytokines, an increase in anti-inflammatory cytokines, and an increase in TGF-ß and maintenance of the cellular redox state with a consequent reduction in levels of inflammatory infiltrate and concomitant stimulation of type III collagen gene expression, as well as a decrease in the size of the wound in square centimeter 6 days after the injury. Only the combination of therapies was able to favor the process of tissue regeneration due to the development of an approach capable of acting at different stages of the regenerative process, through the mechanisms of action of interventions on the inflammatory process by avoiding its stagnation and stimulating progression of regeneration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / Terapia com Luz de Baixa Intensidade / Diabetes Mellitus Experimental / Camundongos Endogâmicos C57BL Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Inflammation Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / Terapia com Luz de Baixa Intensidade / Diabetes Mellitus Experimental / Camundongos Endogâmicos C57BL Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Inflammation Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos