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Random cellulose acetate nanofibers: a breakthrough for cultivated meat production.
Dos Santos, Ana Elisa Antunes; Guadalupe, Jorge Luís; Albergaria, Juliano Douglas Silva; Almeida, Itallo Augusto; Moreira, Amanda Maria Siqueira; Copola, Aline Gonçalves Lio; de Paula, Ana Maria; Neves, Bernardo Ruegger Almeida; Santos, João Paulo Ferreira; da Silva, Aline Bruna; Jorge, Erika Cristina; Andrade, Luciana de Oliveira; de Araújo, Isabella Paula.
Afiliação
  • Dos Santos AEA; Department of Morphology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Guadalupe JL; Department of Morphology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Albergaria JDS; Laboratory of Biomaterials, Department of Materials Engineering, Federal Center for Technological Education of Minas Gerais (CEFET-MG), Belo Horizonte, Brazil.
  • Almeida IA; Department of Morphology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Moreira AMS; Department of Morphology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Copola AGL; Laboratory of Biomaterials, Department of Materials Engineering, Federal Center for Technological Education of Minas Gerais (CEFET-MG), Belo Horizonte, Brazil.
  • de Paula AM; Department of Physics, Institute of Exact Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Neves BRA; Department of Physics, Institute of Exact Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Santos JPF; Laboratory of Biomaterials, Department of Materials Engineering, Federal Center for Technological Education of Minas Gerais (CEFET-MG), Belo Horizonte, Brazil.
  • da Silva AB; Laboratory of Biomaterials, Department of Materials Engineering, Federal Center for Technological Education of Minas Gerais (CEFET-MG), Belo Horizonte, Brazil.
  • Jorge EC; Department of Morphology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Andrade LO; Department of Morphology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • de Araújo IP; Laboratory of Biomaterials, Department of Materials Engineering, Federal Center for Technological Education of Minas Gerais (CEFET-MG), Belo Horizonte, Brazil.
Front Nutr ; 10: 1297926, 2023.
Article em En | MEDLINE | ID: mdl-38249608
ABSTRACT
Overcoming the challenge of creating thick, tissue-resembling muscle constructs is paramount in the field of cultivated meat production. This study investigates the remarkable potential of random cellulose acetate nanofibers (CAN) as a transformative scaffold for muscle tissue engineering (MTE), specifically in the context of cultivated meat applications. Through a comparative analysis between random and aligned CAN, utilizing C2C12 and H9c2 myoblasts, we unveil the unparalleled capabilities of random CAN in facilitating muscle differentiation, independent of differentiation media, by exploiting the YAP/TAZ-related mechanotransduction pathway. In addition, we have successfully developed a novel process for stacking cell-loaded CAN sheets, enabling the production of a three-dimensional meat product. C2C12 and H9c2 loaded CAN sheets were stacked (up to four layers) to form a ~300-400 µm thick tissue 2 cm in length, organized in a mesh of uniaxial aligned cells. To further demonstrate the effectiveness of this methodology for cultivated meat purposes, we have generated thick and viable constructs using chicken muscle satellite cells (cSCs) and random CAN. This groundbreaking discovery offers a cost-effective and biomimetic solution for cultivating and differentiating muscle cells, forging a crucial link between tissue engineering and the pursuit of sustainable and affordable cultivated meat production.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Clinical_trials Idioma: En Revista: Front Nutr Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Clinical_trials Idioma: En Revista: Front Nutr Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça