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Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro.
Segatto, Natália V; Simões, Lucas D; Bender, Camila B; Sousa, Fernanda S; Oliveira, Thais L; Paschoal, Júlia D F; Pacheco, Bruna S; Lopes, Isadora; Seixas, Fabiana K; Qazi, Aisha; Thomas, Faith M; Chaki, Sulalita; Robertson, Noah; Newsom, Jordan; Patel, Shovik; Rund, Laurie A; Jordan, Luke R; Bolt, Courtni; Schachtschneider, Kyle M; Schook, Lawrence B; Collares, Tiago V.
Afiliação
  • Segatto NV; Technology Development Center, Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
  • Simões LD; Technology Development Center, Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
  • Bender CB; Technology Development Center, Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
  • Sousa FS; Technology Development Center, Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
  • Oliveira TL; Technology Development Center, Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
  • Paschoal JDF; Technology Development Center, Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
  • Pacheco BS; Technology Development Center, Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
  • Lopes I; Technology Development Center, Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
  • Seixas FK; Technology Development Center, Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
  • Qazi A; Department of Animal Sciences, University of Illinois, Urbana, IL, United States.
  • Thomas FM; Department of Animal Sciences, University of Illinois, Urbana, IL, United States.
  • Chaki S; Department of Animal Sciences, University of Illinois, Urbana, IL, United States.
  • Robertson N; Albion College, Albion, MI, United States.
  • Newsom J; Albion College, Albion, MI, United States.
  • Patel S; Department of Animal Sciences, University of Illinois, Urbana, IL, United States.
  • Rund LA; Department of Animal Sciences, University of Illinois, Urbana, IL, United States.
  • Jordan LR; Department of Animal Sciences, University of Illinois, Urbana, IL, United States.
  • Bolt C; Sus Clinicals Inc., Chicago, IL, United States.
  • Schachtschneider KM; Department of Animal Sciences, University of Illinois, Urbana, IL, United States.
  • Schook LB; Sus Clinicals Inc., Chicago, IL, United States.
  • Collares TV; Sus Clinicals Inc., Chicago, IL, United States.
Front Oncol ; 14: 1323422, 2024.
Article em En | MEDLINE | ID: mdl-38469237
ABSTRACT

Introduction:

Bladder cancer is a common neoplasia of the urinary tract that holds the highest cost of lifelong treatment per patient, highlighting the need for a continuous search for new therapies for the disease. Current bladder cancer models are either imperfect in their ability to translate results to clinical practice (mouse models), or rare and not inducible (canine models). Swine models are an attractive alternative to model the disease due to their similarities with humans on several levels. The Oncopig Cancer Model has been shown to develop tumors that closely resemble human tumors. However, urothelial carcinoma has not yet been studied in this platform.

Methods:

We aimed to develop novel Oncopig bladder cancer cell line (BCCL) and investigate whether these urothelial swine cells mimic human bladder cancer cell line (5637 and T24) treatment-responses to cisplatin, doxorubicin, and gemcitabine in vitro.

Results:

Results demonstrated consistent treatment responses between Oncopig and human cells in most concentrations tested (p>0.05). Overall, Oncopig cells were more predictive of T24 than 5637 cell therapeutic responses. Microarray analysis also demonstrated similar alterations in expression of apoptotic (GADD45B and TP53INP1) and cytoskeleton-related genes (ZMYM6 and RND1) following gemcitabine exposure between 5637 (human) and Oncopig BCCL cells, indicating apoptosis may be triggered through similar signaling pathways. Molecular docking results indicated that swine and humans had similar Dg values between the chemotherapeutics and their target proteins.

Discussion:

Taken together, these results suggest the Oncopig could be an attractive animal to model urothelial carcinoma due to similarities in in vitro therapeutic responses compared to human cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Oncol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Oncol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça